IAI FigSearch
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yamashita, K.
Right arrow Articles by Yano, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamashita, K.
Right arrow Articles by Yano, A.

 Previous Article  |  Next Article 

Infection and Immunity, October 1998, p. 4651-4655, Vol. 66, No. 10
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cytotoxic T-Lymphocyte-Mediated Lysis of Toxoplasma gondii-Infected Target Cells Does Not Lead to Death of Intracellular Parasites

Keizo Yamashita,1 Katsuyuki Yui,1,* Masakatsu Ueda,1 and Akihiko Yano2

Department of Medical Zoology, Nagasaki University School of Medicine, 1-12-4 Sakamoto, Nagasaki, Nagasaki 852-8523,1 and Department of Parasitology, Chiba University School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670,2 Japan

Received 22 December 1997/Returned for modification 19 February 1998/Accepted 23 July 1998

CD8+ T cells play a crucial role in the control of infection with intracellular microbes. The mechanisms underlying the CD8+ T-cell-mediated clearance of the intracellular pathogen Toxoplasma gondii are, however, not completely understood. The effect of CD8+ cytotoxic T-lymphocyte (CTL)-mediated lysis of host cells on the viability of intracellular T. gondii was investigated. Quantitative competitive PCR of the gene encoding T. gondii major surface antigen (SAG-1) was combined with treatment of the parasites with DNase, which removed the DNA template of nonviable parasites. The induction by CD8+ CTLs of apoptosis in cells infected with T. gondii did not result in the reduction of live parasites, indicating that intracellular T. gondii remains alive after lysis of host cells by CTLs.

* Corresponding author. Mailing address: Department of Medical Zoology, Nagasaki University School of Medicine, 1-12-4 Sakamoto, Nagasaki, Nagasaki 852-8523, Japan. Phone: 81-95-849-7070. Fax: 81-95-849-7073. E-mail: katsu{at}net.nagasaki-u.ac.jp.

Infection and Immunity, October 1998, p. 4651-4655, Vol. 66, No. 10
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. J. Virol. Eukaryot. Cell
Microbiol. Mol. Biol. Rev. Clin. Vaccine Immunol. All ASM Journals

Copyright © 1998 by the American Society for Microbiology. All rights reserved.