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Infection and Immunity, October 1998, p. 4748-4754, Vol. 66, No. 10
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparison of the PspA Sequence from Streptococcus pneumoniae EF5668 to the Previously Identified PspA Sequence from Strain Rx1 and Ability of PspA from EF5668 To Elicit Protection against Pneumococci of Different Capsular Types

Larry S. McDaniel,1,2,3,* D. Olga McDaniel,2 Susan K. Hollingshead,3 and David E. Briles3,4

Departments of Microbiology1 and Surgery,2 The University of Mississippi Medical Center, Jackson, Mississippi 39216, and Departments of Microbiology,3 Comparative Medicine, and Pediatrics,4 The University of Alabama at Birmingham, Birmingham, Alabama 35294

Received 20 April 1998/Returned for modification 2 June 1998/Accepted 24 July 1998

PspA (pneumococcal surface protein A) is a serologically varied virulence factor of Streptococcus pneumoniae. In mice, PspA has been shown to elicit an antibody response that protects against fatal challenge with encapsulated S. pneumoniae, and the protection-eliciting residues have been mapped to the alpha -helical N-terminal half of the protein. To date, a published DNA sequence for pspA is available only for S. pneumoniae Rx1, a laboratory strain. PspA/EF5668 (EF5668 indicates the strain of origin of the PspA) is serologically distinct from PspA/Rx1. Sequencing of the gene encoding PspA/EF5668 revealed 71% identity with that of PspA/Rx1. The greatest amount of divergence between the two proteins was seen in their alpha -helical portions, which are surface exposed and probably under selective pressure to diversify serologically. In spite of the diversity within the alpha -helical regions of PspAs, we have observed that recombinant PspA (rPspA)/EF5668, like rPspA/Rx1, can elicit cross-protection against pneumococci of different capsular and PspA serological types.

* Corresponding author. Mailing address: Department of Microbiology, The University of Mississippi Medical Center, 2500 North State St., Jackson, MS 39216. Phone: (601) 984-6880. Fax: (601) 984-1708. E-mail: LSMCD{at}fiona.umsmed.edu.

Infection and Immunity, October 1998, p. 4748-4754, Vol. 66, No. 10
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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