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Infection and Immunity, October 1998, p. 4832-4837, Vol. 66, No. 10
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Urease Plays an Important Role in the Chemotactic Motility of Helicobacter pylori in a Viscous Environment

Hiroki Nakamura,1,2 Hironori Yoshiyama,1 Hiroaki Takeuchi,1 Tomoko Mizote,1,3 Kiwamu Okita,2 and Teruko Nakazawa1,*

Department of Microbiology1 and First Department of Internal Medicine,2 Yamaguchi University School of Medicine, Ube, Yamaguchi 755-8505, and Department of Environmental Science, Yamaguchi Prefectural University, Yamaguchi 753-0011,3 Japan

Received 27 March 1998/Returned for modification 6 May 1998/Accepted 8 July 1998

Helicobacter pylori exhibits chemotactic responses to urea, flurofamide, acetohydroxamic acid, and sodium bicarbonate. In buffer, the chemotactic activities of a urease-positive strain were higher than those of the isogenic urease-negative strain. Moreover, the chemotactic activities of the urease-positive strain were increased in a viscous solution containing 3% polyvinylpyrrolidone, whereas those of the urease-negative mutant were not. These results are in accordance with the fact that the mutant strain did not show swarming in motility agar regardless of having flagella. Incubation of the wild-type strain with flurofamide resulted in partial inhibition of the chemotactic activities in the viscous solution. In addition, incubation with acetohydroxamic acid, a low-molecular-weight, diffusible urease inhibitor, resulted in complete loss of chemotactic activity in the viscous solution. The inhibition of the chemotactic activity by urease inhibitors paralleled the inhibition of urease. The chemotactic activity of H. pylori was also inhibited by the proton carrier carbonyl cyanide m-chlorophenylhydrazone, showing that H. pylori utilizes proton motive force for motility. These results indicate that cytoplasmic urease plays an important role in the chemotactic motility of H. pylori under a condition that mimics the ecological niche of the bacterium, the gastric mucous layer.

* Corresponding author. Mailing address: Department of Microbiology, Yamaguchi University School of Medicine, Ube, Yamaguchi 755-8505, Japan. Phone: (81)836-22-2226. Fax: (81)836-22-2415. E-mail: nakazawa{at}po.cc.yamaguchi-u.ac.jp.

Infection and Immunity, October 1998, p. 4832-4837, Vol. 66, No. 10
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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