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Infection and Immunity, October 1998, p. 4856-4866, Vol. 66, No. 10
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Experimental Infection of Mongolian Gerbils with Wild-Type and Mutant Helicobacter pylori Strains

Hans-Peter Wirth,1,2 Michael H. Beins,1 Manqiao Yang,1,2 Kyi T. Tham,3,4 and Martin J. Blaser1,4,*

Division of Infectious Diseases, Department of Medicine,1 and Department of Pathology,3 Vanderbilt University School of Medicine, and Department of Veterans Affairs Medical Center,4 Nashville, Tennessee, and Division of Gastroenterology, University Hospital, Zurich, Switzerland2

Received 5 March 1998/Returned for modification 22 June 1998/Accepted 22 July 1998

Experimental Helicobacter pylori infection was studied in Mongolian gerbils with fresh human isolates that carry or do not carry cagA (cagA-positive or cagA-negative, respectively), multiply passaged laboratory strains, wild-type strain G1.1, or isogenic ureA, cagA, or vacA mutants of G1.1. Animals were sacrificed 1 to 32 weeks after challenge, the stomach was removed from each animal for quantitative culture, urease test, and histologic testing, and blood was collected for antibody determinations. No colonization occurred after >= 20 in vitro passages of wild-type strain G1.1 or with the ureA mutant of G1.1. In contrast, infection occurred in animals challenged with wild-type G1.1 (99 of 101 animals) or the cagA (25 of 25) or vacA (25 of 29) mutant of G1.1. Infection with G1.1 persisted for at least 8 months. All 15 animals challenged with any of three fresh human cagA-positive isolates became infected, in contrast to only 6 (23%) of 26 animals challenged with one of four fresh human cagA-negative isolates (P < 0.001). Similar to infection in humans, H. pylori colonization of gerbils induced gastric inflammation and a systemic antibody response to H. pylori antigens. These data confirm the utility of gerbils as an animal model of H. pylori infection and indicate the importance of bacterial strain characteristics for successful infection.

* Corresponding author. Mailing address: Vanderbilt University School of Medicine, A-3310 Medical Center North, Nashville, TN 37232-2605. Phone: (615) 322-2035. Fax: (615) 343-6160. E-mail: Martin.Blaser{at}mcmail.vanderbilt.edu.

Infection and Immunity, October 1998, p. 4856-4866, Vol. 66, No. 10
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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