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Infection and Immunity, October 1998, p. 4867-4874, Vol. 66, No. 10
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Murine Dendritic Cells Pulsed In Vitro with Toxoplasma gondii Antigens Induce Protective Immunity In Vivo

Isabelle Bourguin,1,2,* Muriel Moser,2 Dominique Buzoni-Gatel,1 Françoise Tielemans,2 Daniel Bout,1 Jacques Urbain,2 and Oberdan Leo2

CJF INSERM 93-09 d'Immunologie des Maladies Infectieuses, Equipe Associée INRA d'Immunologie Parasitaire, U.F.R. des Sciences Pharmaceutiques, 37200 Tours, France,1 and Laboratoire de Physiologie Animale, Département de Biologie Moléculaire, Université Libre de Bruxelles, 1640 Rhode-St-Genèse, Belgium2

Received 6 April 1998/Returned for modification 19 May 1998/Accepted 23 July 1998

The activation of a predominant T-helper-cell subset plays a critical role in disease resolution. In the case of Toxoplasma gondii, the available evidence indicates that CD4+ protective cells belong to the Th1 subset. The aim of this study was to determine whether T. gondii antigens (in T. gondii sonicate [TSo]) presented by splenic dendritic cells (DC) were able to induce a specific immune response in vivo and to protect CBA/J mice orally challenged with T. gondii cysts. CBA/J mice immunized with TSo-pulsed DC exhibited significantly fewer cysts in their brains after oral infection with T. gondii 76K than control mice did. Protected mice developed a strong humoral response in vivo, with especially high levels of anti-TSo immunoglobulin G2a antibodies in serum. T. gondii antigens such as SAG1 (surface protein), SAG2 (surface protein), MIC1 (microneme protein), ROP2 through ROP4 (rhoptry proteins), and MIC2 (microneme protein) were recognized predominantly. Furthermore, DC loaded with TSo, which synthesized high levels of interleukin-12 (IL-12), triggered a strong cellular response in vivo, as assessed by the proliferation of lymph node cells in response to TSo restimulation in vitro. Cellular proliferation was associated with gamma interferon and IL-2 production. Taken together, these results indicate that immunization of CBA/J mice with TSo-pulsed DC can induce both humoral and Th1-like cellular immune responses and affords partial resistance against the establishment of chronic toxoplasmosis.

* Corresponding author. Mailing address: CJF INSERM 93-09 d'Immunologie des Maladies Infectieuses, Equipe Associée INRA d'Immunologie Parasitaire, U.F.R. des Sciences Pharmaceutiques, 31, avenue Monge, 37200 Tours, France. Phone: 33 2 47 36 71 85. Fax: 33 2 47 36 72 52. E-mail: bourguin{at}univ-tours.fr.

Infection and Immunity, October 1998, p. 4867-4874, Vol. 66, No. 10
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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