This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Godfroid, F.
Right arrow Articles by Letesson, J.-J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Godfroid, F.
Right arrow Articles by Letesson, J.-J.

 Previous Article  |  Next Article 

Infection and Immunity, November 1998, p. 5485-5493, Vol. 66, No. 11
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification of the Perosamine Synthetase Gene of Brucella melitensis 16M and Involvement of Lipopolysaccharide O Side Chain in Brucella Survival in Mice and in Macrophages

Fabrice Godfroid,1,* Bernard Taminiau,1 Isabelle Danese,1 Philippe Denoel,1 Anne Tibor,1 Vincent Weynants,1 Axel Cloeckaert,2 Jacques Godfroid,3 and Jean-Jacques Letesson1

Unité de Recherche en Biologie Moléculaire (URBM), Laboratoire d'Immunologie et de Microbiologie, Facultés Universitaires Notre Dame de la Paix, Namur,1 and Centre d'Étude et de Recherche Vétérinaire et Agrochimique (CERVA), Brussels,3 Belgium, and Institut National de la Recherche Agronomique, Centre de Recherche de Tours, Nouzilly, France2

Received 2 March 1998/Returned for modification 27 May 1998/Accepted 14 August 1998

Brucella organisms are facultative intracellular bacteria that may infect many species of animals as well as humans. The smooth lipopolysaccharide (S-LPS) has been reported to be an important virulence factor of these organisms, but the genetic basis of expression of the S-LPS O antigen has not yet been described. Likewise, the role of the O side chain of S-LPS in the survival of Brucella has not been clearly defined. A mini-Tn5 transposon mutant library of Brucella melitensis 16M was screened by enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies (MAbs) directed against the O side chain of Brucella. One mutant, designated B3B2, failed to express any O side chain as confirmed by ELISA, Western blot analysis, and colony coloration with crystal violet. Nucleotide sequence analysis demonstrated that the transposon disrupted an open reading frame with significant homology to the putative perosamine synthetase genes of Vibrio cholerae O1 and Escherichia coli O157:H7. The low G+C content of this DNA region suggests that this gene may have originated from a species other than a Brucella sp. The survival of B. melitensis mutant strain B3B2 in the mouse model and in bovine macrophages was examined. The results suggested that S-LPS or, more precisely, its O side chain is essential for survival in mice but not in macrophages.

* Corresponding author. Mailing address: Unité de Recherche en Biologie Moléculaire (URBM), Laboratoire d'Immunologie et de Microbiologie, Facultés Universitaire Notre Dame de la Paix, 61 rue de Bruxelles, B-5000, Namur, Belgium. Phone: 32-81-72 44 03. Fax: 32-81-72 44 20. E-mail: Fabrice.Godefroid{at}fundp.ac.be.

Infection and Immunity, November 1998, p. 5485-5493, Vol. 66, No. 11
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ilg, K., Endt, K., Misselwitz, B., Stecher, B., Aebi, M., Hardt, W.-D. (2009). O-Antigen-Negative Salmonella enterica Serovar Typhimurium Is Attenuated in Intestinal Colonization but Elicits Colitis in Streptomycin-Treated Mice. Infect. Immun. 77: 2568-2575 [Abstract] [Full Text]  
  • Bourg, G., Sube, R., O'Callaghan, D., Patey, G. (2009). Interactions between Brucella suis VirB8 and Its Homolog TraJ from the Plasmid pSB102 Underline the Dynamic Nature of Type IV Secretion Systems. J. Bacteriol. 191: 2985-2992 [Abstract] [Full Text]  
  • Rajashekara, G., Covert, J., Petersen, E., Eskra, L., Splitter, G. (2008). Genomic Island 2 of Brucella melitensis Is a Major Virulence Determinant: Functional Analyses of Genomic Islands. J. Bacteriol. 190: 6243-6252 [Abstract] [Full Text]  
  • Sheng, H., Lim, J. Y., Watkins, M. K., Minnich, S. A., Hovde, C. J. (2008). Characterization of an Escherichia coli O157:H7 O-Antigen Deletion Mutant and Effect of the Deletion on Bacterial Persistence in the Mouse Intestine and Colonization at the Bovine Terminal Rectal Mucosa. Appl. Environ. Microbiol. 74: 5015-5022 [Abstract] [Full Text]  
  • Liautard, J., Ouahrani-Bettache, S., Jubier-Maurin, V., Lafont, V., Kohler, S., Liautard, J.-P. (2007). Identification and Isolation of Brucella suis Virulence Genes Involved in Resistance to the Human Innate Immune System. Infect. Immun. 75: 5167-5174 [Abstract] [Full Text]  
  • Caro-Hernandez, P., Fernandez-Lago, L., de Miguel, M.-J., Martin-Martin, A. I., Cloeckaert, A., Grillo, M.-J., Vizcaino, N. (2007). Role of the Omp25/Omp31 Family in Outer Membrane Properties and Virulence of Brucella ovis. Infect. Immun. 75: 4050-4061 [Abstract] [Full Text]  
  • Posadas, D. M., Martin, F. A., Sabio y Garcia, J. V., Spera, J. M., Delpino, M. V., Baldi, P., Campos, E., Cravero, S. L., Zorreguieta, A. (2007). The TolC Homologue of Brucella suis Is Involved in Resistance to Antimicrobial Compounds and Virulence. Infect. Immun. 75: 379-389 [Abstract] [Full Text]  
  • Pei, J., Turse, J. E., Wu, Q., Ficht, T. A. (2006). Brucella abortus Rough Mutants Induce Macrophage Oncosis That Requires Bacterial Protein Synthesis and Direct Interaction with the Macrophage.. Infect. Immun. 74: 2667-2675 [Abstract] [Full Text]  
  • Lavigne, J.-P., Patey, G., Sangari, F. J., Bourg, G., Ramuz, M., O'Callaghan, D., Michaux-Charachon, S. (2005). Identification of a New Virulence Factor, BvfA, in Brucella suis. Infect. Immun. 73: 5524-5529 [Abstract] [Full Text]  
  • Jimenez de Bagues, M. P., Gross, A., Terraza, A., Dornand, J. (2005). Regulation of the Mitogen-Activated Protein Kinases by Brucella spp. Expressing a Smooth and Rough Phenotype: Relationship to Pathogen Invasiveness. Infect. Immun. 73: 3178-3183 [Abstract] [Full Text]  
  • Danese, I., Haine, V., Delrue, R.-M., Tibor, A., Lestrate, P., Stevaux, O., Mertens, P., Paquet, J.-Y., Godfroid, J., De Bolle, X., Letesson, J.-J. (2004). The Ton System, an ABC Transporter, and a Universally Conserved GTPase Are Involved in Iron Utilization by Brucella melitensis 16M. Infect. Immun. 72: 5783-5790 [Abstract] [Full Text]  
  • Jimenez de Bagues, M. P., Terraza, A., Gross, A., Dornand, J. (2004). Different Responses of Macrophages to Smooth and Rough Brucella spp.: Relationship to Virulence. Infect. Immun. 72: 2429-2433 [Abstract] [Full Text]  
  • Nascimento, A. L. T. O., Ko, A. I., Martins, E. A. L., Monteiro-Vitorello, C. B., Ho, P. L., Haake, D. A., Verjovski-Almeida, S., Hartskeerl, R. A., Marques, M. V., Oliveira, M. C., Menck, C. F. M., Leite, L. C. C., Carrer, H., Coutinho, L. L., Degrave, W. M., Dellagostin, O. A., El-Dorry, H., Ferro, E. S., Ferro, M. I. T., Furlan, L. R., Gamberini, M., Giglioti, E. A., Goes-Neto, A., Goldman, G. H., Goldman, M. H. S., Harakava, R., Jeronimo, S. M. B, Junqueira-de-Azevedo, I. L. M., Kimura, E. T., Kuramae, E. E., Lemos, E. G. M., Lemos, M. V. F., Marino, C. L., Nunes, L. R., de Oliveira, R. C., Pereira, G. G., Reis, M. S., Schriefer, A., Siqueira, W. J., Sommer, P., Tsai, S. M., Simpson, A. J. G., Ferro, J. A., Camargo, L. E. A., Kitajima, J. P., Setubal, J. C., Van Sluys, M. A. (2004). Comparative Genomics of Two Leptospira interrogans Serovars Reveals Novel Insights into Physiology and Pathogenesis. J. Bacteriol. 186: 2164-2172 [Abstract] [Full Text]  
  • Pei, J., Ficht, T. A. (2004). Brucella abortus Rough Mutants Are Cytopathic for Macrophages in Culture. Infect. Immun. 72: 440-450 [Abstract] [Full Text]  
  • Lestrate, P., Dricot, A., Delrue, R.-M., Lambert, C., Martinelli, V., De Bolle, X., Letesson, J.-J., Tibor, A. (2003). Attenuated Signature-Tagged Mutagenesis Mutants of Brucella melitensis Identified during the Acute Phase of Infection in Mice. Infect. Immun. 71: 7053-7060 [Abstract] [Full Text]  
  • Rittig, M. G., Kaufmann, A., Robins, A., Shaw, B., Sprenger, H., Gemsa, D., Foulongne, V., Rouot, B., Dornand, J. (2003). Smooth and rough lipopolysaccharide phenotypes of Brucella induce different intracellular trafficking and cytokine/chemokine release in human monocytes. J. Leukoc. Biol. 74: 1045-1055 [Abstract] [Full Text]  
  • Monreal, D., Grillo, M. J., Gonzalez, D., Marin, C. M., De Miguel, M. J., Lopez-Goni, I., Blasco, J. M., Cloeckaert, A, Moriyon, I. (2003). Characterization of Brucella abortus O-Polysaccharide and Core Lipopolysaccharide Mutants and Demonstration that a Complete Core Is Required for Rough Vaccines To Be Efficient against Brucella abortus and Brucella ovis in the Mouse Model. Infect. Immun. 71: 3261-3271 [Abstract] [Full Text]  
  • Fernandez-Prada, C. M., Zelazowska, E. B., Nikolich, M., Hadfield, T. L., Roop II, R. M., Robertson, G. L., Hoover, D. L. (2003). Interactions between Brucella melitensis and Human Phagocytes: Bacterial Surface O-Polysaccharide Inhibits Phagocytosis, Bacterial Killing, and Subsequent Host Cell Apoptosis. Infect. Immun. 71: 2110-2119 [Abstract] [Full Text]  
  • Porte, F., Naroeni, A., Ouahrani-Bettache, S., Liautard, J.-P. (2003). Role of the Brucella suis Lipopolysaccharide O Antigen in Phagosomal Genesis and in Inhibition of Phagosome-Lysosome Fusion in Murine Macrophages. Infect. Immun. 71: 1481-1490 [Abstract] [Full Text]  
  • Ko, J., Splitter, G. A. (2003). Molecular Host-Pathogen Interaction in Brucellosis: Current Understanding and Future Approaches to Vaccine Development for Mice and Humans. Clin. Microbiol. Rev. 16: 65-78 [Abstract] [Full Text]  
  • Kohler, S., Foulongne, V., Ouahrani-Bettache, S., Bourg, G., Teyssier, J., Ramuz, M., Liautard, J.-P. (2002). The analysis of the intramacrophagic virulome of Brucella suis deciphers the environment encountered by the pathogen inside the macrophage host cell. Proc. Natl. Acad. Sci. USA 99: 15711-15716 [Abstract] [Full Text]  
  • Tibor, A., Wansard, V., Bielartz, V., Delrue, R.-M., Danese, I., Michel, P., Walravens, K., Godfroid, J., Letesson, J.-J. (2002). Effect of omp10 or omp19 Deletion on Brucella abortus Outer Membrane Properties and Virulence in Mice. Infect. Immun. 70: 5540-5546 [Abstract] [Full Text]  
  • Paulsen, I. T., Seshadri, R., Nelson, K. E., Eisen, J. A., Heidelberg, J. F., Read, T. D., Dodson, R. J., Umayam, L., Brinkac, L. M., Beanan, M. J., Daugherty, S. C., Deboy, R. T., Durkin, A. S., Kolonay, J. F., Madupu, R., Nelson, W. C., Ayodeji, B., Kraul, M., Shetty, J., Malek, J., Van Aken, S. E., Riedmuller, S., Tettelin, H., Gill, S. R., White, O., Salzberg, S. L., Hoover, D. L., Lindler, L. E., Halling, S. M., Boyle, S. M., Fraser, C. M. (2002). The Brucellasuis genome reveals fundamental similarities between animal and plant pathogens and symbionts. Proc. Natl. Acad. Sci. USA 99: 13148-13153 [Abstract] [Full Text]  
  • Taminiau, B., Daykin, M., Swift, S., Boschiroli, M.-L., Tibor, A., Lestrate, P., De Bolle, X., O'Callaghan, D., Williams, P., Letesson, J.-J. (2002). Identification of a Quorum-Sensing Signal Molecule in the Facultative Intracellular Pathogen Brucella melitensis. Infect. Immun. 70: 3004-3011 [Abstract] [Full Text]  
  • Kohler, S., Ekaza, E., Paquet, J.-Y., Walravens, K., Teyssier, J., Godfroid, J., Liautard, J.-P. (2002). Induction of dnaK through Its Native Heat Shock Promoter Is Necessary for Intramacrophagic Replication of Brucella suis. Infect. Immun. 70: 1631-1634 [Abstract] [Full Text]  
  • Albermann, C., Piepersberg, W. (2001). Expression and identification of the RfbE protein from Vibrio cholerae O1 and its use for the enzymatic synthesis of GDP-D-perosamine. Glycobiology 11: 655-661 [Abstract] [Full Text]  
  • Fernandez-Prada, C. M., Nikolich, M., Vemulapalli, R., Sriranganathan, N., Boyle, S. M., Schurig, G. G., Hadfield, T. L., Hoover, D. L. (2001). Deletion of wboA Enhances Activation of the Lectin Pathway of Complement in Brucella abortus and Brucella melitensis. Infect. Immun. 69: 4407-4416 [Abstract] [Full Text]  
  • Ugalde, J. E., Czibener, C., Feldman, M. F., Ugalde, R. A. (2000). Identification and Characterization of the Brucella abortus Phosphoglucomutase Gene: Role of Lipopolysaccharide in Virulence and Intracellular Multiplication. Infect. Immun. 68: 5716-5723 [Abstract] [Full Text]  
  • Vemulapalli, R., He, Y., Buccolo, L. S., Boyle, S. M., Sriranganathan, N., Schurig, G. G. (2000). Complementation of Brucella abortus RB51 with a Functional wboA Gene Results in O-Antigen Synthesis and Enhanced Vaccine Efficacy but No Change in Rough Phenotype and Attenuation. Infect. Immun. 68: 3927-3932 [Abstract] [Full Text]  
  • Hong, P. C., Tsolis, R. M., Ficht, T. A. (2000). Identification of Genes Required for Chronic Persistence of Brucella abortus in Mice. Infect. Immun. 68: 4102-4107 [Abstract] [Full Text]  
  • Ekaza, E., Guilloteau, L., Teyssier, J., Liautard, J.-P., Köhler, S. (2000). Functional analysis of the ClpATPase ClpA of Brucella suis, and persistence of a knockout mutant in BALB/c mice. Microbiology 146: 1605-1616 [Abstract] [Full Text]  
  • Foulongne, V., Bourg, G., Cazevieille, C., Michaux-Charachon, S., O'Callaghan, D. (2000). Identification of Brucella suis Genes Affecting Intracellular Survival in an In Vitro Human Macrophage Infection Model by Signature-Tagged Transposon Mutagenesis. Infect. Immun. 68: 1297-1303 [Abstract] [Full Text]  
  • McQuiston, J. R., Vemulapalli, R., Inzana, T. J., Schurig, G. G., Sriranganathan, N., Fritzinger, D., Hadfield, T. L., Warren, R. A., Snellings, N., Hoover, D., Halling, S. M., Boyle, S. M. (1999). Genetic Characterization of a Tn5-Disrupted Glycosyltransferase Gene Homolog in Brucella abortus and Its Effect on Lipopolysaccharide Composition and Virulence. Infect. Immun. 67: 3830-3835 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»