The EspB Protein of Enteropathogenic Escherichia coli Is Targeted to the Cytoplasm of Infected HeLa Cells

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Infection and Immunity, November 1998, p. 5501-5507, Vol. 66, No. 11
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The EspB Protein of Enteropathogenic Escherichia coli Is Targeted to the Cytoplasm of Infected HeLa Cells

Kathleen A. Taylor,¹ Colin B. O'Connell,¹^,² Paul W. Luther,³ and Michael S. Donnenberg¹^,²^,^*

Division of Infectious Diseases, Department of Medicine,¹ Department of Molecular Microbiology and Immunology,² and Department of Physiology,³ University of Maryland School of Medicine, Baltimore, Maryland 21201

Received 17 February 1998/Returned for modification 18 March 1998/Accepted 21 August 1998

The EspB protein of enteropathogenic Escherichia coli (EPEC) is exported via a type III secretion apparatus. EspB is criticalfor signaling the host cell and for the development of the attachingand effacing lesion characteristic of EPEC infection. We usedcellular fractionation and confocal laser scanning microscopyto determine the cellular location of EspB during infection ofHeLa cells. Both methods indicated that EspB is targeted to thecytoplasm of infected cells. Using mutants, we found that EspBtargeting to the host cell cytoplasm requires the type III secretionapparatus and the secreted proteins EspA and EspD, but not intimin.These results provide insights into the function of the type IIIsecretion apparatus of EPEC and the functions of the Esp proteins.

^* Corresponding author. Mailing address: Division of Infectious Diseases, Department of Medicine, University of Maryland Schoolof Medicine, 10 S. Pine St., MSTF-900, Baltimore, MD 21201. Phone:(410) 706-7560. Fax: (410) 706-8700. E-mail: mdonnenb{at}umaryland.edu.

Infection and Immunity, November 1998, p. 5501-5507, Vol. 66, No. 11
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

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