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Infection and Immunity, November 1998, p. 5561-5564, Vol. 66, No. 11
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Effect of P39 Gene Deletion in Live Brucella Vaccine Strains on Residual Virulence and Protective Activity in Mice

Anne Tibor,1,* Isabelle Jacques,2 Laurence Guilloteau,2 Jean-Michel Verger,2 Maggy Grayon,2 Valerie Wansard,1 and Jean-Jacques Letesson1

Laboratoire de Microbiologie et d'Immunologie, Facultés Universitaires Notre-Dame de la Paix, B-5000 Namur, Belgium,1 and Laboratoire de Pathologie Infectieuse et d'Immunologie, INRA-Tours, Nouzilly, France2

Received 27 April 1998/Returned for modification 2 July 1998/Accepted 30 July 1998

The 39-kilodalton protein (P39) has previously been shown to be an immunodominant protein in Brucella infections. P39 gene deletion mutants of vaccine strains Brucella abortus S19 and Brucella melitensis Rev.1 were constructed by gene replacement. This deletion did not significantly modify the residual virulence of both vaccine strains in CD-1 mice. CD-1 mice vaccinated with the parent or mutant strains were protected against a virulent challenge. Mutant vaccine strains devoid of P39 could provide a means for differentiating vaccinated from infected animals.

* Corresponding author. Mailing address: Laboratoire de Microbiologie et Immunologie, Facultés Universitaires Notre-Dame de la Paix, Rue de Bruxelles 61, B-5000 Namur, Belgium. Phone: 32 81 72 44 44. Fax: 32 81 72 44 20. E-mail: anne.tibor{at}fundp.ac.be.

Infection and Immunity, November 1998, p. 5561-5564, Vol. 66, No. 11
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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