This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sharma, A.
Right arrow Articles by Genco, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sharma, A.
Right arrow Articles by Genco, R. J.

 Previous Article  |  Next Article 

Infection and Immunity, December 1998, p. 5703-5710, Vol. 66, No. 12
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cloning, Expression, and Sequencing of a Cell Surface Antigen Containing a Leucine-Rich Repeat Motif from Bacteroides forsythus ATCC 43037

Ashu Sharma,1,* Hakimuddin T. Sojar,1 Ingrid Glurich,1 Kiyonobu Honma,1 Howard K. Kuramitsu,1,2 and Robert J. Genco1,2

Department of Oral Biology, School of Dental Medicine,1 and Department of Microbiology, School of Medicine and Biomedical Sciences,2 State University of New York at Buffalo, Buffalo, New York 14214

Received 26 May 1998/Returned for modification 17 July 1998/Accepted 17 September 1998

Bacteroides forsythus is a recently recognized human periodontopathogen associated with advanced, as well as recurrent, periodontitis. However, very little is known about the mechanism of pathogenesis of this organism. The present study was undertaken to identify the surface molecules of this bacterium that may play roles in its adherence to oral tissues or triggering of a host immune response(s). The gene (bspA) encoding a cell surface-associated protein of B. forsythus with an apparent molecular mass of 98 kDa was isolated by immunoscreening of a B. forsythus gene library constructed in a lambda ZAP II vector. The encoded 98-kDa protein (BspA) contains 14 complete repeats of 23 amino acid residues that show partial homology to leucine-rich repeat motifs. A recombinant protein containing the repeat region was expressed in Escherichia coli, purified, and utilized for antibody production, as well as in vitro binding studies. The purified recombinant protein bound strongly to fibronectin and fibrinogen in a dose-dependent manner and further inhibited the binding of B. forsythus cells to these extracellular matrix (ECM) components. In addition, adult patients with B. forsythus-associated periodontitis expressed specific antibodies against the BspA protein. We report here the cloning and expression of an immunogenic cell surface-associated protein (BspA) of B. forsythus and speculate that it mediates the binding of bacteria to ECM components and clotting factors (fibronectin and fibrinogen, respectively), which may be important in the colonization of the oral cavity by this bacterium and is also a target for the host immune response.

* Corresponding author. Mailing address: Department of Oral Biology, 208 Foster Hall, 3435 Main St., State University of New York at Buffalo, Buffalo, NY 14214-3092. Phone: (716) 829-3518. Fax: (716) 829-3942. E-mail: sharmaa{at}acsu.buffalo.edu.

Infection and Immunity, December 1998, p. 5703-5710, Vol. 66, No. 12
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Loimaranta, V., Hytonen, J., Pulliainen, A. T., Sharma, A., Tenovuo, J., Stromberg, N., Finne, J. (2009). Leucine-rich Repeats of Bacterial Surface Proteins Serve as Common Pattern Recognition Motifs of Human Scavenger Receptor gp340. J. Biol. Chem. 284: 18614-18623 [Abstract] [Full Text]  
  • Thompson, H., Homer, K. A., Rao, S., Booth, V., Hosie, A. H. F. (2009). An Orthologue of Bacteroides fragilis NanH Is the Principal Sialidase in Tannerella forsythia. J. Bacteriol. 191: 3623-3628 [Abstract] [Full Text]  
  • Dumetz, F., Duchaud, E., Claverol, S., Orieux, N., Papillon, S., Lapaillerie, D., Le Henaff, M. (2008). Analysis of the Flavobacterium psychrophilum outer-membrane subproteome and identification of new antigenic targets for vaccine by immunomics. Microbiology 154: 1793-1801 [Abstract] [Full Text]  
  • Tang, G., Kitten, T., Munro, C. L., Wellman, G. C., Mintz, K. P. (2008). EmaA, a Potential Virulence Determinant of Aggregatibacter actinomycetemcomitans in Infective Endocarditis. Infect. Immun. 76: 2316-2324 [Abstract] [Full Text]  
  • Onishi, S., Honma, K., Liang, S., Stathopoulou, P., Kinane, D., Hajishengallis, G., Sharma, A. (2008). Toll-Like Receptor 2-Mediated Interleukin-8 Expression in Gingival Epithelial Cells by the Tannerella forsythia Leucine-Rich Repeat Protein BspA. Infect. Immun. 76: 198-205 [Abstract] [Full Text]  
  • Sakakibara, J., Nagano, K., Murakami, Y., Higuchi, N., Nakamura, H., Shimozato, K., Yoshimura, F. (2007). Loss of adherence ability to human gingival epithelial cells in S-layer protein-deficient mutants of Tannerella forsythensis. Microbiology 153: 866-876 [Abstract] [Full Text]  
  • Inagaki, S., Onishi, S., Kuramitsu, H. K., Sharma, A. (2006). Porphyromonas gingivalis Vesicles Enhance Attachment, and the Leucine-Rich Repeat BspA Protein Is Required for Invasion of Epithelial Cells by "Tannerella forsythia". Infect. Immun. 74: 5023-5028 [Abstract] [Full Text]  
  • Sharma, A., Inagaki, S., Honma, K., Sfintescu, C., Baker, P.J., Evans, R.T. (2005). Tannerella forsythia-induced Alveolar Bone Loss in Mice Involves Leucine-rich-repeat BspA Protein. JDR 84: 462-467 [Abstract] [Full Text]  
  • Seepersaud, R., Hanniffy, S. B., Mayne, P., Sizer, P., Le Page, R., Wells, J. M. (2005). Characterization of a Novel Leucine-Rich Repeat Protein Antigen from Group B Streptococci That Elicits Protective Immunity. Infect. Immun. 73: 1671-1683 [Abstract] [Full Text]  
  • Ikegami, A., Honma, K., Sharma, A., Kuramitsu, H. K. (2004). Multiple Functions of the Leucine-Rich Repeat Protein LrrA of Treponema denticola. Infect. Immun. 72: 4619-4627 [Abstract] [Full Text]  
  • Ruddy, M. J., Shen, F., Smith, J. B., Sharma, A., Gaffen, S. L. (2004). Interleukin-17 regulates expression of the CXC chemokine LIX/CXCL5 in osteoblasts: implications for inflammation and neutrophil recruitment. J. Leukoc. Biol. 76: 135-144 [Abstract] [Full Text]  
  • Hasebe, A., Yoshimura, A., Into, T., Kataoka, H., Tanaka, S., Arakawa, S., Ishikura, H., Golenbock, D. T., Sugaya, T., Tsuchida, N., Kawanami, M., Hara, Y., Shibata, K.-i. (2004). Biological Activities of Bacteroides forsythus Lipoproteins and Their Possible Pathological Roles in Periodontal Disease. Infect. Immun. 72: 1318-1325 [Abstract] [Full Text]  
  • Sabet, M., Lee, S.-W., Nauman, R. K., Sims, T., Um, H.-S. (2003). The surface (S-) layer is a virulence factor of Bacteroides forsythus. Microbiology 149: 3617-3627 [Abstract] [Full Text]  
  • Ishikura, H., Arakawa, S., Nakajima, T., Tsuchida, N., Ishikawa, I. (2003). Cloning of the Tannerella forsythensis (Bacteroides forsythus) siaHI gene and purification of the sialidase enzyme. J Med Microbiol 52: 1101-1107 [Abstract] [Full Text]  
  • Kuramitsu, H. K. (2003). MOLECULAR GENETIC ANALYSIS OF THE VIRULENCE OF ORAL BACTERIAL PATHOGENS: AN HISTORICAL PERSPECTIVE. CROBM 14: 331-344 [Abstract] [Full Text]  
  • Yoneda, M., Hirofuji, T., Motooka, N., Nozoe, K., Shigenaga, K., Anan, H., Miura, M., Kabashima, H., Matsumoto, A., Maeda, K. (2003). Humoral Immune Responses to S-Layer-Like Proteins of Bacteroides forsythus. CVI 10: 383-387 [Abstract] [Full Text]  
  • Duncan, M. J. (2003). GENOMICS OF ORAL BACTERIA. CROBM 14: 175-187 [Abstract] [Full Text]  
  • Hajishengallis, G., Martin, M., Sojar, H. T., Sharma, A., Schifferle, R. E., DeNardin, E., Russell, M. W., Genco, R. J. (2002). Dependence of Bacterial Protein Adhesins on Toll-Like Receptors for Proinflammatory Cytokine Induction. CVI 9: 403-411 [Abstract] [Full Text]  
  • Honma, K., Kuramitsu, H. K., Genco, R. J., Sharma, A. (2001). Development of a Gene Inactivation System for Bacteroides forsythus: Construction and Characterization of a BspA Mutant. Infect. Immun. 69: 4686-4690 [Abstract] [Full Text]  
  • Fernandez-Prada, C. M., Hoover, D. L., Tall, B. D., Hartman, A. B., Kopelowitz, J., Venkatesan, M. M. (2000). Shigella flexneri IpaH7.8 Facilitates Escape of Virulent Bacteria from the Endocytic Vacuoles of Mouse and Human Macrophages. Infect. Immun. 68: 3608-3619 [Abstract] [Full Text]  
  • Zuckert, W. R., Meyer, J., Barbour, A. G. (1999). Comparative Analysis and Immunological Characterization of the Borrelia Bdr Protein Family. Infect. Immun. 67: 3257-3266 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»