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Infection and Immunity, December 1998, p. 5785-5791, Vol. 66, No. 12
INSERM CJF 97-10,
Received 8 May 1998/Returned for modification 8 July 1998/Accepted 4 September 1998
To study the influence of Helicobacter pylori on
epithelial barrier function, bacteria, bacterial sonicates, or broth
culture supernatants were incubated for 24 h with HT29-19A
intestinal cells grown as monolayers. Subsequently,
the monolayers were mounted in Ussing chambers, and electrical
resistance (R), fluxes of 22Na (JNa) and
14C-mannitol (JMan) (markers of the paracellular pathway),
and fluxes of horseradish peroxidase (HRP) in total
(J3H-HRP), intact (JHRPi), and degraded forms were
measured. H. pylori did not induce any modification of
the paracellular pathway (R = 148 ± 10 versus 174 ± 16
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Helicobacter pylori Alters Exogenous Antigen
Absorption and Processing in a Digestive Tract Epithelial Cell
Line Model
· cm2; JNa = 4.16 ± 0.44 versus
3.51 ± 0.41 µEq/h · cm2; JMan = 0.081 ± 0.01 versus 0.058 ± 0.009 µmol/h · cm2), nor did it modify J3H-HRP (2,201 ±
255 versus 2,110 ± 210 ng/h · cm2 for
H. pylori-infected and control cells, respectively).
However, in the presence of H. pylori, we observed a
significant increase in JHRPi (520 ± 146 versus 171 ± 88 ng/h · cm2). This effect was not dependent of the
cag status of the strain and was not reproduced by the
sonicates or the culture supernatants. It was related to the presence
of urease, since a urease-negative mutant of H. pylori did
not induce this effect. Ammonia and bafilomycin A1, two
agents known to increase the endolysosomal pH, reproduced the increase
in JHRPi. In conclusion, H. pylori does not affect directly
the integrity of intercellular junctions of epithelial cells in vitro,
but it increases the passage of intact HRP, probably by inhibition of
the intralysosomal degradation due to the release of ammonia. The
increased transport of intact macromolecules may contribute to the
induction and maintenance of gastric inflammation by H. pylori.
*
Corresponding author. Mailing address: INSERM CJF
97-10, Faculté Necker Enfants Malades, 156 rue de Vaugirard,
75743 Paris Cedex, France. Phone: 33 1 40 61 56 34. Fax: 33 1 40 61 56 38. E-mail: matysiak{at}necker.fr.
Infection and Immunity, December 1998, p. 5785-5791, Vol. 66, No. 12
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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