This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bhushan, R.
Right arrow Articles by Frasch, C. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bhushan, R.
Right arrow Articles by Frasch, C. E.

 Previous Article  |  Next Article 

Infection and Immunity, December 1998, p. 5848-5853, Vol. 66, No. 12
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Estimation of Group B Streptococcus Type III Polysaccharide-Specific Antibody Concentrations in Human Sera Is Antigen Dependent

Reva Bhushan,* Bascom F. Anthony, and Carl E. Frasch

Division of Bacterial Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892

Received 20 May 1998/Returned for modification 10 July 1998/Accepted 3 September 1998

The presence of immunoglobulin G (IgG) antibodies against group B streptococcus (GBS) type III polysaccharide (PS) has been correlated with protection against GBS disease. The GBS type III PS is structurally similar to the pneumococcal type 14 PS, differing only in the presence of sialic acid residues. Four different preparations of GBS type III PS were evaluated for their specificity in enzyme-linked immunosorbent assay (ELISA): free PS, free PS mixed with methylated human serum albumin (mHSA), PS conjugated to biotin and PS conjugated to human serum albumin. Three groups of human sera were used to evaluate these PS preparations: sera from recipients of a GBS PS vaccine, sera from women receiving a GBS type III PS-tetanus toxoid conjugate vaccine, and sera from nonimmunized healthy women of childbearing age. Estimated antibody concentrations were different depending on the PS preparation used. Using any of the four preparations, we were able to measure <= 0.05 µg of IgG antibody to the GBS type III PS per ml. The specificity of the assay was determined by competitive inhibition with homologous and heterologous PS. The pneumococcal type 14 PS did not inhibit binding of antibody to the native GBS type III PS in sera from adults receiving the GBS PS vaccine or in sera from nonimmunized adults (except serum G9). The pneumococcal type 14 PS inhibited 50% in sera from recipients of GBS type III conjugate vaccine and in serum G9 when GBS type III PS conjugated to biotin or to HSA was used as antigen in ELISA. These data show that free GBS type III PS or PS mixed with mHSA is a sensitive and specific antigen for ELISA and that conjugation can alter the antigenic specificity of a PS.

* Corresponding author. Mailing address: Laboratory of Bacterial Polysaccharides, Division of Bacterial Products, Center for Biologics Evaluation and Research, 29 Lincoln Dr., Bethesda, MD 20892. Phone: (301) 496-9173. Fax: (301) 402-2776. E-mail: vaccine{at}helix.nih.gov.

Infection and Immunity, December 1998, p. 5848-5853, Vol. 66, No. 12
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Burton, R. L., Nahm, M. H. (2006). Development and Validation of a Fourfold Multiplexed Opsonization Assay (MOPA4) for Pneumococcal Antibodies.. CVI 13: 1004-1009 [Abstract] [Full Text]  
  • Lewis, A. L., Nizet, V., Varki, A. (2004). Discovery and characterization of sialic acid O-acetylation in group B Streptococcus. Proc. Natl. Acad. Sci. USA 101: 11123-11128 [Abstract] [Full Text]  
  • Guttormsen, H.-K., Baker, C. J., Nahm, M. H., Paoletti, L. C., Zughaier, S. M., Edwards, M. S., Kasper, D. L. (2002). Type III Group B Streptococcal Polysaccharide Induces Antibodies That Cross-React with Streptococcus pneumoniae Type 14. Infect. Immun. 70: 1724-1738 [Abstract] [Full Text]  
  • Kasper, D. L., Wessels, M. R., Guttormsen, H.-K., Paoletti, L. C., Edwards, M. S., Baker;, C. J., Bhushan, R., Frasch, C. E. (1999). Measurement of Human Antibodies to Type III Group B Streptococcus. Infect. Immun. 67: 4303-4305 [Full Text]  
  • Arakere, G., Flores, A. E., Ferrieri, P., Frasch, C. E. (1999). Inhibition Enzyme-Linked Immunosorbent Assay for Serotyping of Group B Streptococcal Isolates. J. Clin. Microbiol. 37: 2564-2567 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»