This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ASM journals
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Porgador, A.
Right arrow Articles by Kelsall, B. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Porgador, A.
Right arrow Articles by Kelsall, B. L.

 Previous Article  |  Next Article 

Infection and Immunity, December 1998, p. 5876-5881, Vol. 66, No. 12
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Intranasal Immunization with Cytotoxic T-Lymphocyte Epitope Peptide and Mucosal Adjuvant Cholera Toxin: Selective Augmentation of Peptide-Presenting Dendritic Cells in Nasal Mucosa-Associated Lymphoid Tissue

Angel Porgador,1,dagger Herman F. Staats,2 Yasushi Itoh,1 and Brian L. Kelsall3,*

Lymphocyte Biology Section, Laboratory of Immunology,1 and Immune Cell Interaction Unit, Mucosal Immunity Section, Laboratory for Clinical Investigation,3 National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, and Department of Medicine, Center for AIDS Research, Duke University Medical School, Durham, North Carolina2

Received 29 April 1998/Returned for modification 8 June 1998/Accepted 27 August 1998

We previously reported that cholera toxin (CT) was required as a mucosal adjuvant for the induction of peptide-specific cytotoxic T lymphocytes (CTL) following intranasal immunization with CTL epitope peptides (A. Porgador et al., J. Immunol. 158:834-841, 1997). The present study was performed to identify the site and the antigen-presenting cell (APC) population responsible for the presentation of intranasally administered CTL epitope peptide immunogens and to determine whether CT directly affects antigen presentation by these APCs. For these experiments, C57BL/6 mice were intranasally immunized with the ovalbumin H-2Kb-restricted CTL epitope SIINFEKL with or without CT. Cells were then isolated from the cervical lymph nodes (CLN) and the nasal mucosa-associated lymphoid tissue (NALT) and tested for the ability to stimulate the B3Z T-cell hybridoma, which recognizes SIINFEKL in association with H-2Kb. Dendritic cell (DC)-enriched CLN cells from mice immunized with peptide and CT or peptide only could stimulate B3Z cells, while DC-depleted CLN cells from either group were unable to stimulate B3Z cells. NALT cells of mice immunized with peptide and CT, but not with peptide alone, were able to efficiently stimulate B3Z hybridomas. Depletion of N418-positive DC from these NALT cells resulted in significant reduction of B3Z activation. Our results indicate that DC are the APC responsible for the presentation of CTL epitope peptides following intranasal immunization and that CT augments the ability of dendritic cells in the NALT, but not in the draining CLN, to present CLT epitope peptides. This finding suggests that CT acts locally as a mucosal adjuvant and that NALT DC are the predominant APC involved with the induction of immunity after intranasal immunization with peptide immunogens and CT.

* Corresponding author. Mailing address: Immune Cell Interaction Unit, LCI, NIAID, NIH, 10/11N238, 10 Center Dr., Bethesda, MD 20892-1890. Phone: (301) 496-7473. Fax: (301) 402-2240. E-mail: Kelsall{at}nih.gov.

dagger Present address: Department of Virology, Faculty of Medicine, Ben-Gurion University of the Negev, Beer-Sheba, 84105, Israel.

Infection and Immunity, December 1998, p. 5876-5881, Vol. 66, No. 12
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Fahlen-Yrlid, L., Gustafsson, T., Westlund, J., Holmberg, A., Strombeck, A., Blomquist, M., MacPherson, G. G., Holmgren, J., Yrlid, U. (2009). CD11chigh Dendritic Cells Are Essential for Activation of CD4+ T Cells and Generation of Specific Antibodies following Mucosal Immunization. J. Immunol. 183: 5032-5041 [Abstract] [Full Text]  
  • Medaglini, D., Ciabattini, A., Cuppone, A. M., Costa, C., Ricci, S., Costalonga, M., Pozzi, G. (2006). In Vivo Activation of Naive CD4+ T Cells in Nasal Mucosa-Associated Lymphoid Tissue following Intranasal Immunization with Recombinant Streptococcus gordonii. Infect. Immun. 74: 2760-2766 [Abstract] [Full Text]  
  • Grdic, D., Ekman, L., Schon, K., Lindgren, K., Mattsson, J., Magnusson, K.-E., Ricciardi-Castagnoli, P., Lycke, N. (2005). Splenic Marginal Zone Dendritic Cells Mediate the Cholera Toxin Adjuvant Effect: Dependence on the ADP-Ribosyltransferase Activity of the Holotoxin. J. Immunol. 175: 5192-5202 [Abstract] [Full Text]  
  • Eriksson, A. M., Schon, K. M., Lycke, N. Y. (2004). The Cholera Toxin-Derived CTA1-DD Vaccine Adjuvant Administered Intranasally Does Not Cause Inflammation or Accumulate in the Nervous Tissues. J. Immunol. 173: 3310-3319 [Abstract] [Full Text]  
  • Debes, G. F., Bonhagen, K., Wolff, T., Kretschmer, U., Krautwald, S., Kamradt, T., Hamann, A. (2004). CC Chemokine Receptor 7 Expression by Effector/Memory CD4+ T Cells Depends on Antigen Specificity and Tissue Localization during Influenza A Virus Infection. J. Virol. 78: 7528-7535 [Abstract] [Full Text]  
  • Bagley, K. C., Abdelwahab, S. F., Tuskan, R. G., Lewis, G. K. (2003). An Enzymatically Active A Domain Is Required for Cholera-Like Enterotoxins To Induce a Long-Lived Blockade on the Induction of Oral Tolerance: New Method for Screening Mucosal Adjuvants. Infect. Immun. 71: 6850-6856 [Abstract] [Full Text]  
  • Gueirard, P., Ave, P., Balazuc, A.-M., Thiberge, S., Huerre, M., Milon, G., Guiso, N. (2003). Bordetella bronchiseptica Persists in the Nasal Cavities of Mice and Triggers Early Delivery of Dendritic Cells in the Lymph Nodes Draining the Lower and Upper Respiratory Tract. Infect. Immun. 71: 4137-4143 [Abstract] [Full Text]  
  • Balmelli, C., Demotz, S., Acha-Orbea, H., De Grandi, P., Nardelli-Haefliger, D. (2002). Trachea, Lung, and Tracheobronchial Lymph Nodes Are the Major Sites Where Antigen-Presenting Cells Are Detected after Nasal Vaccination of Mice with Human Papillomavirus Type 16 Virus-Like Particles. J. Virol. 76: 12596-12602 [Abstract] [Full Text]  
  • Bagley, K. C., Abdelwahab, S. F., Tuskan, R. G., Fouts, T. R., Lewis, G. K. (2002). Pertussis toxin and the adenylate cyclase toxin from Bordetella pertussis activate human monocyte-derived dendritic cells and dominantly inhibit cytokine production through a cAMP-dependent pathway. J. Leukoc. Biol. 72: 962-969 [Abstract] [Full Text]  
  • Bagley, K. C., Abdelwahab, S. F., Tuskan, R. G., Fouts, T. R., Lewis, G. K. (2002). Cholera Toxin and Heat-Labile Enterotoxin Activate Human Monocyte-Derived Dendritic Cells and Dominantly Inhibit Cytokine Production through a Cyclic AMP-Dependent Pathway. Infect. Immun. 70: 5533-5539 [Abstract] [Full Text]  
  • Bonenfant, C., Dimier-Poisson, I., Velge-Roussel, F., Buzoni-Gatel, D., Del Giudice, G., Rappuoli, R., Bout, D. (2001). Intranasal Immunization with SAG1 and Nontoxic Mutant Heat-Labile Enterotoxins Protects Mice against Toxoplasma gondii. Infect. Immun. 69: 1605-1612 [Abstract] [Full Text]  
  • Simmons, C. P., Hussell, T., Sparer, T., Walzl, G., Openshaw, P., Dougan, G. (2001). Mucosal Delivery of a Respiratory Syncytial Virus CTL Peptide with Enterotoxin-Based Adjuvants Elicits Protective, Immunopathogenic, and Immunoregulatory Antiviral CD8+ T Cell Responses. J. Immunol. 166: 1106-1113 [Abstract] [Full Text]  
  • Bellone, M., Cantarella, D., Castiglioni, P., Crosti, M. C., Ronchetti, A., Moro, M., Garancini, M. P., Casorati, G., Dellabona, P. (2000). Relevance of the Tumor Antigen in the Validation of Three Vaccination Strategies for Melanoma. J. Immunol. 165: 2651-2656 [Abstract] [Full Text]  
  • Simmons, C. P., Mastroeni, P., Fowler, R., Ghaem-maghami, M., Lycke, N., Pizza, M., Rappuoli, R., Dougan, G. (1999). MHC Class I-Restricted Cytotoxic Lymphocyte Responses Induced by Enterotoxin-Based Mucosal Adjuvants. J. Immunol. 163: 6502-6510 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»