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Infection and Immunity, December 1998, p. 5876-5881, Vol. 66, No. 12
Lymphocyte Biology Section,
Received 29 April 1998/Returned for modification 8 June
1998/Accepted 27 August 1998
We previously reported that cholera toxin (CT) was required as a
mucosal adjuvant for the induction of peptide-specific cytotoxic T
lymphocytes (CTL) following intranasal immunization with CTL epitope
peptides (A. Porgador et al., J. Immunol. 158:834-841, 1997). The
present study was performed to identify the site and the
antigen-presenting cell (APC) population responsible for the presentation of intranasally administered CTL epitope peptide immunogens and to determine whether CT directly affects antigen presentation by these APCs. For these experiments, C57BL/6 mice were
intranasally immunized with the ovalbumin H-2Kb-restricted
CTL epitope SIINFEKL with or without CT. Cells were then isolated from
the cervical lymph nodes (CLN) and the nasal mucosa-associated lymphoid
tissue (NALT) and tested for the ability to stimulate the B3Z T-cell
hybridoma, which recognizes SIINFEKL in association with
H-2Kb. Dendritic cell (DC)-enriched CLN cells from mice
immunized with peptide and CT or peptide only could stimulate B3Z
cells, while DC-depleted CLN cells from either group were unable to
stimulate B3Z cells. NALT cells of mice immunized with peptide and CT,
but not with peptide alone, were able to efficiently stimulate B3Z hybridomas. Depletion of N418-positive DC from these NALT cells resulted in significant reduction of B3Z activation. Our results indicate that DC are the APC responsible for the presentation of CTL
epitope peptides following intranasal immunization and that CT augments
the ability of dendritic cells in the NALT, but not in the draining
CLN, to present CLT epitope peptides. This finding suggests that CT
acts locally as a mucosal adjuvant and that NALT DC are the predominant
APC involved with the induction of immunity after intranasal
immunization with peptide immunogens and CT.
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Intranasal Immunization with Cytotoxic T-Lymphocyte
Epitope Peptide and Mucosal Adjuvant Cholera Toxin: Selective
Augmentation of Peptide-Presenting Dendritic Cells in Nasal
Mucosa-Associated Lymphoid Tissue

*
Corresponding author. Mailing address: Immune Cell
Interaction Unit, LCI, NIAID, NIH, 10/11N238, 10 Center Dr., Bethesda, MD 20892-1890. Phone: (301) 496-7473. Fax: (301) 402-2240. E-mail: Kelsall{at}nih.gov.
Present address: Department of Virology, Faculty of Medicine,
Ben-Gurion University of the Negev, Beer-Sheba, 84105, Israel.
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