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Infection and Immunity, December 1998, p. 5964-5971, Vol. 66, No. 12
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Local Cytokine Response in Helicobacter pylori-Infected Subjects

C. Lindholm,1,* M. Quiding-Järbrink,1 H. Lönroth,2 A. Hamlet,1 and A.-M. Svennerholm1

Department of Medical Microbiology and Immunology, Göteborg University,1 and Department of Surgery, Sahlgrenska University Hospital,2 Göteborg, Sweden

Received 24 April 1998/Returned for modification 15 July 1998/Accepted 17 September 1998

The host immune response to Helicobacter pylori infection might be of importance with regard to the outcome of infection by this organism, e.g., to explain why only a proportion of infected subjects develop peptic ulcers. In this study we have analyzed the local response of different cytokines---i.e., the proinflammatory interleukin-1beta , (IL-1beta ), IL-6, tumor necrosis factor alpha, and IL-8; the immunoregulatory gamma interferon (IFN-gamma ); and IL-4; and the anti-inflammatory transforming growth factor beta (TGF-beta )---in antral biopsy specimens from H. pylori-infected duodenal ulcer (DU) patients and asymptomatic (AS) carriers (i.e., with chronic gastritis only). For comparison, biopsy specimens from uninfected healthy individuals were also analyzed. An immunohistochemical technique was used to allow quantification of the cytokine responses as well as identification of the cell types associated with the cytokine expression. We found that the levels of all of the studied cytokines except IL-4 were increased in the H. pylori-infected subjects compared to the levels in the healthy individuals. Our results indicate that the antral cytokine response is of the Th1 type since IFN-gamma , but not IL-4, was up-regulated both in H. pylori-infected DU patients and in AS carriers. However, there were no significant differences in either proinflammatory or immunoregulatory cytokine levels when H. pylori-infected subjects with and without peptic ulcers were compared. Some of the cytokines, particularly IL-1beta and TGF-beta , were also found in the gastric mucosae of healthy, uninfected subjects. We also showed that the gastric epithelium contributes substantially to the antral cytokine response of the proinflammatory cytokines IL-1beta and IL-6 in addition to IL-8.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, Göteborg University, Guldhedsgatan 10A, S-413 46 Göteborg, Sweden. Phone: 46 31 342 62 16. Fax: 46 31 82 69 76. E-mail: catharina.lindholm{at}microbio.gu.se.

Infection and Immunity, December 1998, p. 5964-5971, Vol. 66, No. 12
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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  • Wang, J., Fan, X., Lindholm, C., Bennett, M., O'Connoll, J., Shanahan, F., Brooks, E. G., Reyes, V. E., Ernst, P. B. (2000). Helicobacter pylori Modulates Lymphoepithelial Cell Interactions Leading to Epithelial Cell Damage through Fas/Fas Ligand Interactions. Infect. Immun. 68: 4303-4311 [Abstract] [Full Text]  
  • Tanahashi, T., Kita, M., Kodama, T., Yamaoka, Y., Sawai, N., Ohno, T., Mitsufuji, S., Wei, Y.-P., Kashima, K., Imanishi, J. (2000). Cytokine Expression and Production by Purified Helicobacter pylori Urease in Human Gastric Epithelial Cells. Infect. Immun. 68: 664-671 [Abstract] [Full Text]  
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  • MASTROENI, P, BOWE, F, CAHILL, R, SIMMONS, C, DOUGAN, G (1999). Vaccines against gut pathogens. Gut 45: 633-635 [Full Text]  
  • Ahlstedt, I., Lindholm, C., Lonroth, H., Hamlet, A., Svennerholm, A.-M., Quiding-Jarbrink, M. (1999). Role of Local Cytokines in Increased Gastric Expression of the Secretory Component in Helicobacter pylori Infection. Infect. Immun. 67: 4921-4925 [Abstract] [Full Text]  
  • ZEVERING, Y, JACOB, L, MEYER, T F (1999). Naturally acquired human immune responses against Helicobacter pylori and implications for vaccine development. Gut 45: 465-474 [Full Text]  


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