This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ASM journals
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by George, K. M.
Right arrow Articles by Small, P. L. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by George, K. M.
Right arrow Articles by Small, P. L. C.

 Previous Article  |  Next Article 

Infect Immun, February 1998, p. 587-593, Vol. 66, No. 2
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Partial Purification and Characterization of Biological Effects of a Lipid Toxin Produced by Mycobacterium ulcerans

Kathleen M. George,* Lucia P. Barker, Diane M. Welty, and P. L. C. Small

Microscopy Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840

Received 3 September 1997/Returned for modification 5 November 1997/Accepted 18 November 1997

Organisms in the genus Mycobacterium cause a variety of human diseases. One member of the genus, M. ulcerans, causes a necrotizing skin disease called Buruli ulcer. Buruli ulcer is unique among mycobacterial diseases in that the organisms at the site of infection are extracellular and there is little acute inflammatory response. Previous literature reported the presence of a toxin in the culture supernatant of M. ulcerans which causes a cytopathic effect on the mouse fibroblast cell line L929 in which the adherent cells round up and detach from the tissue culture plate. Here we report partial purification of a lipid toxin from the culture supernatant of M. ulcerans which is capable of causing the cytopathic effect on L929 cells. We also show that this cytopathic effect is a result of cytoskeletal rearrangement. The M. ulcerans toxin does not cause cell death but instead arrests cells in the G1 phase of the cell cycle.

* Corresponding author. Mailing address: Microscopy Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, 903 S. 4th St., Hamilton, MT 59840. Phone: (406) 363-9342. Fax: (406) 363-9371. E-mail: katie_george{at}nih.gov.




This article has been cited by other articles:

  • Simmonds, R. E., Lali, F. V., Smallie, T., Small, P. L. C., Foxwell, B. M. (2009). Mycolactone Inhibits Monocyte Cytokine Production by a Posttranscriptional Mechanism. J. Immunol. 182: 2194-2202 [Abstract] [Full Text]  
  • Sarfo, F. S., Phillips, R. O., Ampadu, E., Sarpong, F., Adentwe, E., Wansbrough-Jones, M. (2009). Dynamics of the Cytokine Response to Mycobacterium ulcerans during Antibiotic Treatment for M. ulcerans Disease (Buruli Ulcer) in Humans. CVI 16: 61-65 [Abstract] [Full Text]  
  • Torrado, E., Adusumilli, S., Fraga, A. G., Small, P. L. C., Castro, A. G., Pedrosa, J. (2007). Mycolactone-Mediated Inhibition of Tumor Necrosis Factor Production by Macrophages Infected with Mycobacterium ulcerans Has Implications for the Control of Infection. Infect. Immun. 75: 3979-3988 [Abstract] [Full Text]  
  • Ranger, B. S., Mahrous, E. A., Mosi, L., Adusumilli, S., Lee, R. E., Colorni, A., Rhodes, M., Small, P. L. C. (2006). Globally Distributed Mycobacterial Fish Pathogens Produce a Novel Plasmid-Encoded Toxic Macrolide, Mycolactone F. Infect. Immun. 74: 6037-6045 [Abstract] [Full Text]  
  • Phillips, R., Horsfield, C., Mangan, J., Laing, K., Etuaful, S., Awuah, P., Nyarko, K., Osei-Sarpong, F., Butcher, P., Lucas, S., Wansbrough-Jones, M. (2006). Cytokine mRNA Expression in Mycobacteriam ulcerans-Infected Human Skin and Correlation with Local Inflammatory Response. Infect. Immun. 74: 2917-2924 [Abstract] [Full Text]  
  • Jandu, N., Ceponis, P. J. M., Kato, S., Riff, J. D., McKay, D. M., Sherman, P. M. (2006). Conditioned Medium from Enterohemorrhagic Escherichia coli-Infected T84 Cells Inhibits Signal Transducer and Activator of Transcription 1 Activation by Gamma Interferon. Infect. Immun. 74: 1809-1818 [Abstract] [Full Text]  
  • Oliveira, M. S., Fraga, A. G., Torrado, E., Castro, A. G., Pereira, J. P., Filho, A. L., Milanezi, F., Schmitt, F. C., Meyers, W. M., Portaels, F., Silva, M. T., Pedrosa, J. (2005). Infection with Mycobacterium ulcerans Induces Persistent Inflammatory Responses in Mice. Infect. Immun. 73: 6299-6310 [Abstract] [Full Text]  
  • WALSH, D. S., MEYERS, W. M., PORTAELS, F., LANE, J. E., MONGKOLSIRICHAIKUL, D., HUSSEM, K., GOSI, P., MYINT, K. S. A. (2005). HIGH RATES OF APOPTOSIS IN HUMAN MYCOBACTERIUM ULCERANS CULTURE-POSITIVE BURULI ULCER SKIN LESIONS. Am J Trop Med Hyg 73: 410-415 [Abstract] [Full Text]  
  • Mve-Obiang, A., Lee, R. E., Umstot, E. S., Trott, K. A., Grammer, T. C., Parker, J. M., Ranger, B. S., Grainger, R., Mahrous, E. A., Small, P. L. C. (2005). A Newly Discovered Mycobacterial Pathogen Isolated from Laboratory Colonies of Xenopus Species with Lethal Infections Produces a Novel Form of Mycolactone, the Mycobacterium ulcerans Macrolide Toxin. Infect. Immun. 73: 3307-3312 [Abstract] [Full Text]  
  • Stinear, T. P., Hong, H., Frigui, W., Pryor, M. J., Brosch, R., Garnier, T., Leadlay, P. F., Cole, S. T. (2005). Common Evolutionary Origin for the Unstable Virulence Plasmid pMUM Found in Geographically Diverse Strains of Mycobacterium ulcerans. J. Bacteriol. 187: 1668-1676 [Abstract] [Full Text]  
  • Daniel, A. K., Lee, R. E., Portaels, F., Small, P. L. C. (2004). Analysis of Mycobacterium Species for the Presence of a Macrolide Toxin, Mycolactone. Infect. Immun. 72: 123-132 [Abstract] [Full Text]  
  • Rondini, S., Mensah-Quainoo, E., Troll, H., Bodmer, T., Pluschke, G. (2003). Development and Application of Real-Time PCR Assay for Quantification of Mycobacterium ulcerans DNA. J. Clin. Microbiol. 41: 4231-4237 [Abstract] [Full Text]  
  • Haussler, S., Rohde, M., von Neuhoff, N., Nimtz, M., Steinmetz, I. (2003). Structural and Functional Cellular Changes Induced by Burkholderia pseudomallei Rhamnolipid. Infect. Immun. 71: 2970-2975 [Abstract] [Full Text]  
  • Marsollier, L., Honore, N., Legras, P., Manceau, A. L., Kouakou, H., Carbonnelle, B., Cole, S. T. (2003). Isolation of Three Mycobacterium ulcerans Strains Resistant to Rifampin after Experimental Chemotherapy of Mice. Antimicrob. Agents Chemother. 47: 1228-1232 [Abstract] [Full Text]  
  • Mve-Obiang, A., Lee, R. E., Portaels, F., Small, P. L. C. (2003). Heterogeneity of Mycolactones Produced by Clinical Isolates of Mycobacterium ulcerans: Implications for Virulence. Infect. Immun. 71: 774-783 [Abstract] [Full Text]  
  • Dega, H., Bentoucha, A., Robert, J., Jarlier, V., Grosset, J. (2002). Bactericidal Activity of Rifampin-Amikacin against Mycobacterium ulcerans in Mice. Antimicrob. Agents Chemother. 46: 3193-3196 [Abstract] [Full Text]  
  • Pahlevan, A. A., Wright, D. J. M., Bradley, L., Smith, C., Foxwell, B. M. J. (2002). Potential of rifamides to inhibit TNF-induced NF-{kappa}B activation. J Antimicrob Chemother 49: 531-534 [Abstract] [Full Text]  
  • Dobos, K. M., Small, P. L., Deslauriers, M., Quinn, F. D., King, C. H. (2001). Mycobacterium ulcerans Cytotoxicity in an Adipose Cell Model. Infect. Immun. 69: 7182-7186 [Abstract] [Full Text]  
  • George, K. M., Pascopella, L., Welty, D. M., Small, P. L. C. (2000). A Mycobacterium ulcerans Toxin, Mycolactone, Causes Apoptosis in Guinea Pig Ulcers and Tissue Culture Cells. Infect. Immun. 68: 877-883 [Abstract] [Full Text]  
  • Pahlevan, A. A., Wright, D. J. M., Andrews, C., George, K. M., Small, P. L. C., Foxwell, B. M. (1999). The Inhibitory Action of Mycobacterium ulcerans Soluble Factor on Monocyte/T Cell Cytokine Production and NF-{kappa}B Function. J. Immunol. 163: 3928-3935 [Abstract] [Full Text]  
  • George, K. M., Chatterjee, D., Gunawardana, G., Welty, D., Hayman, J., Lee, R., Small, P. L. (1999). Mycolactone: A Polyketide Toxin from Mycobacterium ulcerans Required for Virulence. Science 283: 854-857 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»