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Infect Immun, February 1998, p. 849-852, Vol. 66, No. 2
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Use of Representational Difference Analysis To Identify Genomic Differences between Pathogenic Strains of Vibrio cholerae

Kerstin E. Calia,1 Matthew K. Waldor,2 and Stephen B. Calderwood1,3,*

Division of Infectious Diseases, Massachusetts General Hospital,1 Division of Geographic Medicine and Infectious Diseases, New England Medical Center,2 and Department of Microbiology and Molecular Genetics, Harvard Medical School,3 Boston, Massachusetts

Received 24 September 1997/Returned for modification 27 October 1997/Accepted 20 November 1997

Representational difference analysis (RDA) is a recently developed technique used for amplifying genetic differences between two closely related genomes. We compared RDA and a modified version of RDA to examine genomic differences between the two Vibrio cholerae serogroups that cause epidemic cholera, O1 and O139, and between the two biotypes of the O1 serogroup. With both techniques, we recovered several sequences known to be found only in V. cholerae O139 but absent in its presumed progenitor, V. cholerae O1 El Tor. A greater number of unique fragments were generated in comparing the two V. cholerae O1 biotypes, consistent with the probable greater genetic differences between the two biotypes.

* Corresponding author. Mailing address: Division of Infectious Diseases, Massachusetts General Hospital, 55 Fruit St., Jackson 5, Boston, MA 02114. Phone: (617) 726-3811. Fax: (617) 726-7416. E-mail: calderwood.stephen{at}mgh.harvard.edu.




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