This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vanet, A.
Right arrow Articles by Labigne, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vanet, A.
Right arrow Articles by Labigne, A.

 Previous Article  |  Next Article 

Infect Immun, March 1998, p. 1023-1027, Vol. 66, No. 3
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Evidence for Specific Secretion Rather than Autolysis in the Release of Some Helicobacter pylori Proteins

Anne Vanetdagger and Agnès Labigne*

Unité de Pathogénie Bactérienne des Muqueuses, Institut Pasteur, 75724 Paris Cedex 15, France

Received 29 August 1997/Returned for modification 4 November 1997/Accepted 30 December 1997

We investigated whether Helicobacter pylori cells actively secrete proteins such as the urease subunits UreA and UreB and the GroES and GroEL homologs HspA and HspB or whether these proteins were present in the extracellular compartment as a consequence of autolysis. Using a subcellular fractionation approach associated with quantitative Western blot analyses, we showed that the supernatant protein profiles were very different from those of the cell pellets, even for bacteria harvested in the late growth phase; this suggests that the release process is selective. A typical cytoplasmic protein, a beta -galactosidase homolog, was found exclusively associated with the pellet of whole-cell extracts, and no traces were found in the supernatant. In contrast, UreA, UreB, HspA, and HspB were mostly found in the pellet but significant amounts were also present in the supernatant. HspA and UreB were released into the supernatant at the same rate throughout the growth phase (3%), whereas large portions of HspB and UreA were released during the stationary phase (over 30 and 20%, respectively) rather than during the early growth phase (20% and 6, respectively). The profiles of protein obtained after water extraction of the bacteria with those of the proteins naturally released within the liquid culture supernatants demonstrated that water extraction led to the release of a large amount of protein due to artifactual lysis. Our data support the conclusion that a specific and selective mechanism(s) is involved in the secretion of some H. pylori antigens. A programmed autolysis process does not seem to make a major contribution.

* Corresponding author. Mailing address: Unité de Pathogénie Bactérienne des Muqueuses, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France. Phone: 33 1 40 61 32 73. Fax: 33 1 40 61 36 40. E-mail: alabigne{at}pasteur.fr.

dagger Present address: Institut de Biologie Physico-Chimique, UPR 9073 du C.N.R.S., 75005 Paris, France.




This article has been cited by other articles:

  • Celli, J. P., Turner, B. S., Afdhal, N. H., Keates, S., Ghiran, I., Kelly, C. P., Ewoldt, R. H., McKinley, G. H., So, P., Erramilli, S., Bansil, R. (2009). Helicobacter pylori moves through mucus by reducing mucin viscoelasticity. Proc. Natl. Acad. Sci. USA 106: 14321-14326 [Abstract] [Full Text]  
  • Gao, L., Michel, A., Weck, M. N., Arndt, V., Pawlita, M., Brenner, H. (2009). Helicobacter pylori Infection and Gastric Cancer Risk: Evaluation of 15 H. pylori Proteins Determined by Novel Multiplex Serology. Cancer Res. 69: 6164-6170 [Abstract] [Full Text]  
  • Ge, Y., Rikihisa, Y. (2007). Surface-Exposed Proteins of Ehrlichia chaffeensis. Infect. Immun. 75: 3833-3841 [Abstract] [Full Text]  
  • Lin, Y.-F., Chen, C.-Y., Tsai, M.-H., Wu, M.-S., Wang, Y.-C., Chuang, E. Y., Lin, J.-T., Yang, P.-C., Chow, L.-P. (2007). Duodenal Ulcer-related Antigens from Helicobacter pylori: Immunoproteome and Protein Microarray Approaches. Mol. Cell. Proteomics 6: 1018-1026 [Abstract] [Full Text]  
  • Kusters, J. G., van Vliet, A. H. M., Kuipers, E. J. (2006). Pathogenesis of Helicobacter pylori Infection. Clin. Microbiol. Rev. 19: 449-490 [Abstract] [Full Text]  
  • Lee, B.-Y., Horwitz, M. A., Clemens, D. L. (2006). Identification, Recombinant Expression, Immunolocalization in Macrophages, and T-Cell Responsiveness of the Major Extracellular Proteins of Francisella tularensis. Infect. Immun. 74: 4002-4013 [Abstract] [Full Text]  
  • Beswick, E. J., Pinchuk, I. V., Minch, K., Suarez, G., Sierra, J. C., Yamaoka, Y., Reyes, V. E. (2006). The Helicobacter pylori Urease B Subunit Binds to CD74 on Gastric Epithelial Cells and Induces NF-{kappa}B Activation and Interleukin-8 Production. Infect. Immun. 74: 1148-1155 [Abstract] [Full Text]  
  • Bergonzelli, G. E., Granato, D., Pridmore, R. D., Marvin-Guy, L. F., Donnicola, D., Corthesy-Theulaz, I. E. (2006). GroEL of Lactobacillus johnsonii La1 (NCC 533) Is Cell Surface Associated: Potential Role in Interactions with the Host and the Gastric Pathogen Helicobacter pylori. Infect. Immun. 74: 425-434 [Abstract] [Full Text]  
  • Gobert, A. P., Bambou, J.-C., Werts, C., Balloy, V., Chignard, M., Moran, A. P., Ferrero, R. L. (2004). Helicobacter pylori Heat Shock Protein 60 Mediates Interleukin-6 Production by Macrophages via a Toll-like Receptor (TLR)-2-, TLR-4-, and Myeloid Differentiation Factor 88-independent Mechanism. J. Biol. Chem. 279: 245-250 [Abstract] [Full Text]  
  • Minnick, M. F., Smitherman, L. S., Samuels, D. S. (2003). Mitogenic Effect of Bartonella bacilliformis on Human Vascular Endothelial Cells and Involvement of GroEL. Infect. Immun. 71: 6933-6942 [Abstract] [Full Text]  
  • Fossati, G., Izzo, G., Rizzi, E., Gancia, E., Modena, D., Moras, M. L., Niccolai, N., Giannozzi, E., Spiga, O., Bono, L., Marone, P., Leone, E., Mangili, F., Harding, S., Errington, N., Walters, C., Henderson, B., Roberts, M. M., Coates, A. R. M., Casetta, B., Mascagni, P. (2003). Mycobacterium tuberculosis Chaperonin 10 Is Secreted in the Macrophage Phagosome: Is Secretion Due to Dissociation and Adoption of a Partially Helical Structure at the Membrane?. J. Bacteriol. 185: 4256-4267 [Abstract] [Full Text]  
  • Kim, N., Weeks, D. L., Shin, J. M., Scott, D. R., Young, M. K., Sachs, G. (2002). Proteins Released by Helicobacter pylori In Vitro. J. Bacteriol. 184: 6155-6162 [Abstract] [Full Text]  
  • Bumann, D., Aksu, S., Wendland, M., Janek, K., Zimny-Arndt, U., Sabarth, N., Meyer, T. F., Jungblut, P. R. (2002). Proteome Analysis of Secreted Proteins of the Gastric Pathogen Helicobacter pylori. Infect. Immun. 70: 3396-3403 [Abstract] [Full Text]  
  • Gobert, A. P., Mersey, B. D., Cheng, Y., Blumberg, D. R., Newton, J. C., Wilson, K. T. (2002). Cutting Edge: Urease Release by Helicobacter pylori Stimulates Macrophage Inducible Nitric Oxide Synthase. J. Immunol. 168: 6002-6006 [Abstract] [Full Text]  
  • Atanassov, C., Pezennec, L., d'Alayer, J., Grollier, G., Picard, B., Fauchere, J.-L. (2002). Novel Antigens of Helicobacter pylori Correspond to Ulcer-Related Antibody Pattern of Sera from Infected Patients. J. Clin. Microbiol. 40: 547-552 [Abstract] [Full Text]  
  • Tannas, T., Dekker, N., Bukholm, G., Bijlsma, J. J. E., Appelmelk, B. J. (2001). Phase Variation in the Helicobacter pylori Phospholipase A Gene and Its Role in Acid Adaptation. Infect. Immun. 69: 7334-7340 [Abstract] [Full Text]  
  • Tullius, M. V., Harth, G., Horwitz, M. A. (2001). High Extracellular Levels of Mycobacterium tuberculosis Glutamine Synthetase and Superoxide Dismutase in Actively Growing Cultures Are Due to High Expression and Extracellular Stability Rather than to a Protein-Specific Export Mechanism. Infect. Immun. 69: 6348-6363 [Abstract] [Full Text]  
  • Dahl, J. L., Wei, J., Moulder, J. W., Laal, S., Friedman, R. L. (2001). Subcellular Localization of the Intracellular Survival-Enhancing Eis Protein of Mycobacterium tuberculosis. Infect. Immun. 69: 4295-4302 [Abstract] [Full Text]  
  • Balachandran, P., Hollingshead, S. K., Paton, J. C., Briles, D. E. (2001). The Autolytic Enzyme LytA of Streptococcus pneumoniae Is Not Responsible for Releasing Pneumolysin. J. Bacteriol. 183: 3108-3116 [Abstract] [Full Text]  
  • Hennequin, C., Porcheray, F., Waligora-Dupriet, A.-J., Collignon, A., Barc, M.-C., Bourlioux, P., Karjalainen, T. (2001). GroEL (Hsp60) of Clostridium difficile is involved in cell adherence. Microbiology 147: 87-96 [Abstract] [Full Text]  
  • OLIARO, J., JOHNSON, R. D., CHEN, W., CHADWICK, V. S., MURRAY, A. (2000). Identification of an immunogenic 18-kDa protein of Helicobacter pylori by alkaline phosphatase gene fusions. J Med Microbiol 49: 643-650 [Abstract] [Full Text]  
  • Yamaguchi, H., Osaki, T., Kai, M., Taguchi, H., Kamiya, S. (2000). Immune Response against a Cross-Reactive Epitope on the Heat Shock Protein 60 Homologue of Helicobacter pylori. Infect. Immun. 68: 3448-3454 [Abstract] [Full Text]  
  • Windle, H. J., Fox, A., Ni Eidhin, D., Kelleher, D. (2000). The Thioredoxin System of Helicobacter pylori. J. Biol. Chem. 275: 5081-5089 [Abstract] [Full Text]  
  • Schraw, W., McClain, M. S., Cover, T. L. (1999). Kinetics and Mechanisms of Extracellular Protein Release by Helicobacter pylori. Infect. Immun. 67: 5247-5252 [Abstract] [Full Text]  
  • Doig, P., de Jonge, B. L., Alm, R. A., Brown, E. D., Uria-Nickelsen, M., Noonan, B., Mills, S. D., Tummino, P., Carmel, G., Guild, B. C., Moir, D. T., Vovis, G. F., Trust, T. J. (1999). Helicobacter pylori Physiology Predicted from Genomic Comparison of Two Strains. Microbiol. Mol. Biol. Rev. 63: 675-707 [Abstract] [Full Text]  
  • Krishnamurthy, P., Parlow, M., Zitzer, J. B., Vakil, N. B., Mobley, H. L. T., Levy, M., Phadnis, S. H., Dunn, B. E. (1998). Helicobacter pylori Containing Only Cytoplasmic Urease Is Susceptible to Acid. Infect. Immun. 66: 5060-5066 [Abstract] [Full Text]  
  • Huesca, M., Goodwin, A., Bhagwansingh, A., Hoffman, P., Lingwood, C. A. (1998). Characterization of an Acidic-pH-Inducible Stress Protein (hsp70), a Putative Sulfatide Binding Adhesin, from Helicobacter pylori. Infect. Immun. 66: 4061-4067 [Abstract] [Full Text]  
  • Icatlo Jr., F. C., Kuroki, M., Kobayashi, C., Yokoyama, H., Ikemori, Y., Hashi, T., Kodama, Y. (1998). Affinity Purification of Helicobacter pylori Urease. RELEVANCE TO GASTRIC MUCIN ADHERENCE BY UREASE PROTEIN. J. Biol. Chem. 273: 18130-18138 [Abstract] [Full Text]  
  • Cao, P., McClain, M. S., Forsyth, M. H., Cover, T. L. (1998). Extracellular Release of Antigenic Proteins by Helicobacter pylori. Infect. Immun. 66: 2984-2986 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»