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Infect Immun, March 1998, p. 1237-1243, Vol. 66, No. 3
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

M-Cell Surface beta 1 Integrin Expression and Invasin-Mediated Targeting of Yersinia pseudotuberculosis to Mouse Peyer's Patch M Cells

M. Ann Clark,* Barry H. Hirst, and Mark A. Jepsondagger

Department of Physiological Sciences, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom

Received 18 August 1997/Returned for modification 28 October 1997/Accepted 1 December 1997

Quantitative analysis of Yersinia pseudotuberculosis infection of murine gut loops revealed that significantly more wild-type bacteria associated with Peyer's patch M cells than with dome enterocytes or goblet cells. An invasin-deficient mutant was significantly attenuated for M-cell invasion, while beta 1 integrin expression was demonstrated in the apical membranes of M cells but not enterocytes. M-cell targeting by Yersinia pseudotuberculosis in vivo may, therefore, be mediated primarily by the interaction of invasin with cell surface beta 1 integrins.

* Corresponding author. Mailing address: Department of Physiological Sciences, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom. Phone: 44 191 222 7772. Fax: 44 191 222 6706. E-mail: Ann.Clark{at}newcastle.ac.uk.

dagger Present address: Cell Imaging Facility and Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom.




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