This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Casey, T. A. Articles by Whipp, S. C. Search for Related Content PubMed PubMed Citation Articles by Casey, T. A. Articles by Whipp, S. C.

 Previous Article  |  Next Article 

Infect Immun, March 1998, p. 1270-1272, Vol. 66, No. 3
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Expression of Heat-Stable Enterotoxin STb by Adherent Escherichia coli Is Not Sufficient To Cause Severe Diarrhea in Neonatal Pigs

Thomas A. Casey,^* Cheryl J. Herring, Robert A. Schneider, Brad T. Bosworth, and Shannon C. Whipp

Enteric Diseases and Food Safety Research Unit, National Animal Disease Center, USDA Agricultural Research Service, Ames, Iowa

Received 2 September 1997/Returned for modification 1 October 1997/Accepted 30 December 1997

The role of Escherichia coli heat-stable enterotoxin B (STb) in neonatal porcine diarrhea caused by enterotoxigenic E. coli was examined by comparing adherent isogenic strains with or without STb. The cloned STb gene (in the plasmid pRAS1) was electroporated into a nonenterotoxigenic strain (226M) which expresses the F41 adhesin. Strain 226M pRAS1 adhered and expressed STb in vivo, causing fluid secretion in ligated ileal loops in neonatal pigs. Although strain 226M pRAS1 caused very mild diarrhea in some orally inoculated neonatal pigs, the weight loss in these pigs was similar to that caused by the parental strain without STb. We conclude that STb does not significantly contribute to diarrhea caused by enterotoxigenic E. coli in neonatal pigs.

---

^* Corresponding author. Mailing address: USDA, ARS, National Animal Disease Center, P.O. Box 70, Ames, IA 50010. Phone: (515) 239-8376. Fax: (515) 239-8458. E-mail: tcasey{at}nadc.ars.usda.gov.

This article has been cited by other articles:

• Casey, T. A., Bosworth, B. T. (2009). Design and evaluation of a multiplex polymerase chain reaction assay for the simultaneous identification of genes for nine different virulence factors associated with Escherichia coli that cause diarrhea and edema disease in swine. jvdi 21: 25-30 [Abstract] [Full Text]  
• Zhang, W., Berberov, E. M., Freeling, J., He, D., Moxley, R. A., Francis, D. H. (2006). Significance of heat-stable and heat-labile enterotoxins in porcine colibacillosis in an additive model for pathogenicity studies.. Infect. Immun. 74: 3107-3114 [Abstract] [Full Text]  
• Berberov, E. M., Zhou, Y., Francis, D. H., Scott, M. A., Kachman, S. D., Moxley, R. A. (2004). Relative Importance of Heat-Labile Enterotoxin in the Causation of Severe Diarrheal Disease in the Gnotobiotic Piglet Model by a Strain of Enterotoxigenic Escherichia coli That Produces Multiple Enterotoxins. Infect. Immun. 72: 3914-3924 [Abstract] [Full Text]  
• Fournout, S., Dozois, C. M., Odin, M., Desautels, C., Peres, S., Herault, F., Daigle, F., Segafredo, C., Laffitte, J., Oswald, E., Fairbrother, J. M., Oswald, I. P. (2000). Lack of a Role of Cytotoxic Necrotizing Factor 1 Toxin from Escherichia coli in Bacterial Pathogenicity and Host Cytokine Response in Infected Germfree Piglets. Infect. Immun. 68: 839-847 [Abstract] [Full Text]