This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Denis, O.
Right arrow Articles by Huygen, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Denis, O.
Right arrow Articles by Huygen, K.

 Previous Article  |  Next Article 

Infect Immun, April 1998, p. 1527-1533, Vol. 66, No. 4
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Vaccination with Plasmid DNA Encoding Mycobacterial Antigen 85A Stimulates a CD4+ and CD8+ T-Cell Epitopic Repertoire Broader than That Stimulated by Mycobacterium tuberculosis H37Rv Infection

Olivier Denis,1 Audrey Tanghe,1 Kamiel Palfliet,1 Fabienne Jurion,1 Thierry-P. van den Berg,2 Albert Vanonckelen,1 Josette Ooms,1 Eric Saman,3 Jeffrey B. Ulmer,4 Jean Content,1 and Kris Huygen1,*

Department of Virology, Pasteur Institute of Brussels,1 and Veterinary and Agrochemical Research Center (VAR),2 1180 Brussels, and Innogenetics Ghent, 9052 Ghent,3 Belgium, and Merck Research Laboratories, West Point, Pennsylvania 194864

Received 15 September 1997/Returned for modification 22 October 1997/Accepted 20 January 1998

Vaccination of mice with plasmid DNA carrying the gene for the major secreted mycobacterial antigen 85A (Ag85A) from Mycobacterium tuberculosis is a powerful technique for generating robust specific Th1 helper T-cell responses, CD8+-mediated cytotoxicity, and protection against M. tuberculosis challenge (K. Huygen et al., Nat. Med. 2:893-898, 1996). We have now analyzed in more detail the antigen-specific immune CD4+- and CD8+-T-cell responses induced in BALB/c mice vaccinated with Ag85A DNA and have compared these responses to those generated by intravenous infection with M. tuberculosis. T-cell-epitope mapping, as measured by interleukin-2 and gamma interferon secretion from splenic T cells restimulated in vitro with synthetic 20-mer peptides spanning the complete mature sequence of Ag85A, demonstrated that DNA vaccination stimulated a stronger and broader T-cell response than did M. tuberculosis infection. Moreover, elevated cytotoxic T lymphocyte (CTL) activity against Ag85A-transfected and peptide-pulsed P815 target cells could be generated exclusively by vaccination with plasmid DNA, not following M. tuberculosis infection. By using DNA vaccination, three Ag85A CTL epitopes with predicted major histocompatibility complex class I binding motifs were defined. One of them was previously reported as a dominant, promiscuously recognized T-cell epitope in healthy humans with primary infections. These data strengthen the potential of DNA vaccination with respect to inducing antituberculous immunity in humans.

* Corresponding author. Mailing address: Pasteur Institute of Brussels, Department Virology, 642 Engelandstraat, 1180 Brussels, Belgium. Phone: 32.2.373.33.70. Fax: 32.2.373.31.74. E-mail: chuygen{at}ben.vub.ac.be.




This article has been cited by other articles:

  • Mir, F.-A., Kaufmann, S. H. E., Eddine, A. N. (2009). A Multicistronic DNA Vaccine Induces Significant Protection against Tuberculosis in Mice and Offers Flexibility in the Expressed Antigen Repertoire. CVI 16: 1467-1475 [Abstract] [Full Text]  
  • Woodworth, J. S., Fortune, S. M., Behar, S. M. (2008). Bacterial Protein Secretion Is Required for Priming of CD8+ T Cells Specific for the Mycobacterium tuberculosis Antigen CFP10. Infect. Immun. 76: 4199-4205 [Abstract] [Full Text]  
  • Wu, Y., Woodworth, J. S., Shin, D. S., Morris, S., Behar, S. M. (2008). Vaccine-Elicited 10-Kilodalton Culture Filtrate Protein-Specific CD8+ T Cells Are Sufficient To Mediate Protection against Mycobacterium tuberculosis Infection. Infect. Immun. 76: 2249-2255 [Abstract] [Full Text]  
  • Sridhar, S., Reyes-Sandoval, A., Draper, S. J., Moore, A. C., Gilbert, S. C., Gao, G. P., Wilson, J. M., Hill, A. V. S. (2008). Single-Dose Protection against Plasmodium berghei by a Simian Adenovirus Vector Using a Human Cytomegalovirus Promoter Containing Intron A. J. Virol. 82: 3822-3833 [Abstract] [Full Text]  
  • Radosevic, K., Wieland, C. W., Rodriguez, A., Weverling, G. J., Mintardjo, R., Gillissen, G., Vogels, R., Skeiky, Y. A. W., Hone, D. M., Sadoff, J. C., van der Poll, T., Havenga, M., Goudsmit, J. (2007). Protective Immune Responses to a Recombinant Adenovirus Type 35 Tuberculosis Vaccine in Two Mouse Strains: CD4 and CD8 T-Cell Epitope Mapping and Role of Gamma Interferon. Infect. Immun. 75: 4105-4115 [Abstract] [Full Text]  
  • Majlessi, L., Combaluzier, B., Albrecht, I., Garcia, J. E., Nouze, C., Pieters, J., Leclerc, C. (2007). Inhibition of Phagosome Maturation by Mycobacteria Does Not Interfere with Presentation of Mycobacterial Antigens by MHC Molecules. J. Immunol. 179: 1825-1833 [Abstract] [Full Text]  
  • Roupie, V., Romano, M., Zhang, L., Korf, H., Lin, M. Y., Franken, K. L. M. C., Ottenhoff, T. H. M., Klein, M. R., Huygen, K. (2007). Immunogenicity of Eight Dormancy Regulon-Encoded Proteins of Mycobacterium tuberculosis in DNA-Vaccinated and Tuberculosis-Infected Mice. Infect. Immun. 75: 941-949 [Abstract] [Full Text]  
  • Sharma, A., Saha, A., Bhattacharjee, S., Majumdar, S., Das Gupta, S. K. (2006). Specific and Randomly Derived Immunoactive Peptide Mimotopes of Mycobacterial Antigens. CVI 13: 1143-1154 [Abstract] [Full Text]  
  • Caccamo, N., Meraviglia, S., La Mendola, C., Guggino, G., Dieli, F., Salerno, A. (2006). Phenotypical and Functional Analysis of Memory and Effector Human CD8 T Cells Specific for Mycobacterial Antigens. J. Immunol. 177: 1780-1785 [Abstract] [Full Text]  
  • D'Souza, S., Romano, M., Korf, J., Wang, X.-M., Adnet, P.-Y., Huygen, K. (2006). Partial Reconstitution of the CD4+-T-Cell Compartment in CD4 Gene Knockout Mice Restores Responses to Tuberculosis DNA Vaccines.. Infect. Immun. 74: 2751-2759 [Abstract] [Full Text]  
  • Rosseels, V., Marche, S., Roupie, V., Govaerts, M., Godfroid, J., Walravens, K., Huygen, K. (2006). Members of the 30- to 32-Kilodalton Mycolyl Transferase Family (Ag85) from Culture Filtrate of Mycobacterium avium subsp. paratuberculosis Are Immunodominant Th1-Type Antigens Recognized Early upon Infection in Mice and Cattle. Infect. Immun. 74: 202-212 [Abstract] [Full Text]  
  • Ko, H.-J., Ko, S.-Y., Kim, Y.-J., Lee, E.-G., Cho, S.-N., Kang, C.-Y. (2005). Optimization of Codon Usage Enhances the Immunogenicity of a DNA Vaccine Encoding Mycobacterial Antigen Ag85B. Infect. Immun. 73: 5666-5674 [Abstract] [Full Text]  
  • Shin, S. J., Chang, C.-F., Chang, C.-D., McDonough, S. P., Thompson, B., Yoo, H. S., Chang, Y.-F. (2005). In Vitro Cellular Immune Responses to Recombinant Antigens of Mycobacterium avium subsp. paratuberculosis. Infect. Immun. 73: 5074-5085 [Abstract] [Full Text]  
  • Kamath, A. B., Woodworth, J., Xiong, X., Taylor, C., Weng, Y., Behar, S. M. (2004). Cytolytic CD8+ T Cells Recognizing CFP10 Are Recruited to the Lung after Mycobacterium tuberculosis Infection. JEM 200: 1479-1489 [Abstract] [Full Text]  
  • Suzuki, M., Aoshi, T., Nagata, T., Koide, Y. (2004). Identification of Murine H2-Dd- and H2-Ab-Restricted T-Cell Epitopes on a Novel Protective Antigen, MPT51, of Mycobacterium tuberculosis. Infect. Immun. 72: 3829-3837 [Abstract] [Full Text]  
  • Romano, M., Denis, O., D'Souza, S., Wang, X.-M., Ottenhoff, T. H. M., Brulet, J.-M., Huygen, K. (2004). Induction of In Vivo Functional Db-Restricted Cytolytic T Cell Activity against a Putative Phosphate Transport Receptor of Mycobacterium tuberculosis. J. Immunol. 172: 6913-6921 [Abstract] [Full Text]  
  • Miki, K., Nagata, T., Tanaka, T., Kim, Y.-H., Uchijima, M., Ohara, N., Nakamura, S., Okada, M., Koide, Y. (2004). Induction of Protective Cellular Immunity against Mycobacterium tuberculosis by Recombinant Attenuated Self-Destructing Listeria monocytogenes Strains Harboring Eukaryotic Expression Plasmids for Antigen 85 Complex and MPB/MPT51. Infect. Immun. 72: 2014-2021 [Abstract] [Full Text]  
  • Taracha, E. L. N., Bishop, R., Musoke, A. J., Hill, A. V. S., Gilbert, S. C. (2003). Heterologous Priming-Boosting Immunization of Cattle with Mycobacterium tuberculosis 85A Induces Antigen-Specific T-Cell Responses. Infect. Immun. 71: 6906-6914 [Abstract] [Full Text]  
  • Majlessi, L., Rojas, M.-J., Brodin, P., Leclerc, C. (2003). CD8+-T-Cell Responses of Mycobacterium-Infected Mice to a Newly Identified Major Histocompatibility Complex Class I-Restricted Epitope Shared by Proteins of the ESAT-6 Family. Infect. Immun. 71: 7173-7177 [Abstract] [Full Text]  
  • Goonetilleke, N. P., McShane, H., Hannan, C. M., Anderson, R. J., Brookes, R. H., Hill, A. V. S. (2003). Enhanced Immunogenicity and Protective Efficacy Against Mycobacterium tuberculosis of Bacille Calmette-Guerin Vaccine Using Mucosal Administration and Boosting with a Recombinant Modified Vaccinia Virus Ankara. J. Immunol. 171: 1602-1609 [Abstract] [Full Text]  
  • Huygen, K. (2003). On the Use of DNA Vaccines for the Prophylaxis of Mycobacterial Diseases. Infect. Immun. 71: 1613-1621 [Full Text]  
  • D'Souza, S., Rosseels, V., Romano, M., Tanghe, A., Denis, O., Jurion, F., Castiglione, N., Vanonckelen, A., Palfliet, K., Huygen, K. (2003). Mapping of Murine Th1 Helper T-Cell Epitopes of Mycolyl Transferases Ag85A, Ag85B, and Ag85C from Mycobacterium tuberculosis. Infect. Immun. 71: 483-493 [Abstract] [Full Text]  
  • D'Souza, S., Rosseels, V., Denis, O., Tanghe, A., De Smet, N., Jurion, F., Palfliet, K., Castiglioni, N., Vanonckelen, A., Wheeler, C., Huygen, K. (2002). Improved Tuberculosis DNA Vaccines by Formulation in Cationic Lipids. Infect. Immun. 70: 3681-3688 [Abstract] [Full Text]  
  • McShane, H., Behboudi, S., Goonetilleke, N., Brookes, R., Hill, A. V. S. (2002). Protective Immunity against Mycobacterium tuberculosis Induced by Dendritic Cells Pulsed with both CD8+- and CD4+-T-Cell Epitopes from Antigen 85A. Infect. Immun. 70: 1623-1626 [Abstract] [Full Text]  
  • Al-Mariri, A., Tibor, A., Mertens, P., De Bolle, X., Michel, P., Godfroid, J., Walravens, K., Letesson, J.-J. (2001). Induction of Immune Response in BALB/c Mice with a DNA Vaccine Encoding Bacterioferritin or P39 of Brucella spp.. Infect. Immun. 69: 6264-6270 [Abstract] [Full Text]  
  • Feng, C. G., Palendira, U., Demangel, C., Spratt, J. M., Malin, A. S., Britton, W. J. (2001). Priming by DNA Immunization Augments Protective Efficacy of Mycobacterium bovis Bacille Calmette-Guerin against Tuberculosis. Infect. Immun. 69: 4174-4176 [Abstract] [Full Text]  
  • Coler, R. N., Campos-Neto, A., Ovendale, P., Day, F. H., Fling, S. P., Zhu, L., Serbina, N., Flynn, J. L., Reed, S. G., Alderson, M. R. (2001). Vaccination with the T Cell Antigen Mtb 8.4 Protects Against Challenge with Mycobacterium tuberculosis. J. Immunol. 166: 6227-6235 [Abstract] [Full Text]  
  • Tanghe, A., D'Souza, S., Rosseels, V., Denis, O., Ottenhoff, T. H. M., Dalemans, W., Wheeler, C., Huygen, K. (2001). Improved Immunogenicity and Protective Efficacy of a Tuberculosis DNA Vaccine Encoding Ag85 by Protein Boosting. Infect. Immun. 69: 3041-3047 [Abstract] [Full Text]  
  • Feng, C. G., Demangel, C., Kamath, A. T., Macdonald, M., Britton, W. J. (2001). Dendritic cells infected with Mycobacterium bovis bacillus Calmette Guerin activate CD8+ T cells with specificity for a novel mycobacterial epitope. Int Immunol 13: 451-458 [Abstract] [Full Text]  
  • Mogues, T., Goodrich, M. E., Ryan, L., LaCourse, R., North, R. J. (2001). The Relative Importance of T Cell Subsets in Immunity and Immunopathology of Airborne Mycobacterium tuberculosis Infection in Mice. JEM 193: 271-280 [Abstract] [Full Text]  
  • Smith, S. M., Brookes, R., Klein, M. R., Malin, A. S., Lukey, P. T., King, A. S., Ogg, G. S., Hill, A. V. S., Dockrell, H. M. (2000). Human CD8+ CTL Specific for the Mycobacterial Major Secreted Antigen 85A. J. Immunol. 165: 7088-7095 [Abstract] [Full Text]  
  • Smith, S. M., Klein, M. R., Malin, A. S., Sillah, J., Huygen, K., Andersen, P., McAdam, K. P. W. J., Dockrell, H. M. (2000). Human CD8+ T Cells Specific for Mycobacterium tuberculosis Secreted Antigens in Tuberculosis Patients and Healthy BCG-Vaccinated Controls in The Gambia. Infect. Immun. 68: 7144-7148 [Abstract] [Full Text]  
  • Geluk, A., van Meijgaarden, K. E., Franken, K. L. M. C., Drijfhout, J. W., D'Souza, S., Necker, A., Huygen, K., Ottenhoff, T. H. M. (2000). Identification of Major Epitopes of Mycobacterium tuberculosis AG85B That Are Recognized by HLA-A*0201-Restricted CD8+ T Cells in HLA-Transgenic Mice and Humans. J. Immunol. 165: 6463-6471 [Abstract] [Full Text]  
  • Tanghe, A., Denis, O., Lambrecht, B., Motte, V., van den Berg, T., Huygen, K. (2000). Tuberculosis DNA Vaccine Encoding Ag85A Is Immunogenic and Protective When Administered by Intramuscular Needle Injection but Not by Epidermal Gene Gun Bombardment. Infect. Immun. 68: 3854-3860 [Abstract] [Full Text]  
  • Mustafa, A. S., Shaban, F. A., Abal, A. T., Al-Attiyah, R., Wiker, H. G., Lundin, K. E. A., Oftung, F., Huygen, K. (2000). Identification and HLA Restriction of Naturally Derived Th1-Cell Epitopes from the Secreted Mycobacterium tuberculosis Antigen 85B Recognized by Antigen-Specific Human CD4+ T-Cell Lines. Infect. Immun. 68: 3933-3940 [Abstract] [Full Text]  
  • Serbina, N. V., Liu, C.-C., Scanga, C. A., Flynn, J. L. (2000). CD8+ CTL from Lungs of Mycobacterium tuberculosis-Infected Mice Express Perforin In Vivo and Lyse Infected Macrophages. J. Immunol. 165: 353-363 [Abstract] [Full Text]  
  • Lo-Man, R., Langeveld, J. P. M., Deriaud, E., Jehanno, M., Rojas, M., Clement, J.-M., Meloen, R. H., Hofnung, M., Leclerc, C. (2000). Extending the CD4+ T-Cell Epitope Specificity of the Th1 Immune Response to an Antigen Using a Salmonella enterica Serovar Typhimurium Delivery Vehicle. Infect. Immun. 68: 3079-3089 [Abstract] [Full Text]  
  • Lefevre, P., Denis, O., De Wit, L., Tanghe, A., Vandenbussche, P., Content, J., Huygen, K. (2000). Cloning of the Gene Encoding a 22-Kilodalton Cell Surface Antigen of Mycobacterium bovis BCG and Analysis of Its Potential for DNA Vaccination against Tuberculosis. Infect. Immun. 68: 1040-1047 [Abstract] [Full Text]  
  • Belperron, A. A., Feltquate, D., Fox, B. A., Horii, T., Bzik, D. J. (1999). Immune Responses Induced by Gene Gun or Intramuscular Injection of DNA Vaccines That Express Immunogenic Regions of the Serine Repeat Antigen from Plasmodium falciparum. Infect. Immun. 67: 5163-5169 [Abstract] [Full Text]  
  • Smith, S. M., Malin, A. S., Pauline T., , Lukey, , Atkinson, S. E., Content, J., Huygen, K., Dockrell, H. M. (1999). Characterization of Human Mycobacterium bovis Bacille Calmette-Guerin-Reactive CD8+ T Cells. Infect. Immun. 67: 5223-5230 [Abstract] [Full Text]  
  • Rodrigues, M. M., Ribeirao, M., Pereira-Chioccola, V., Renia, L., Costa, F. (1999). Predominance of CD4 Th1 and CD8 Tc1 Cells Revealed by Characterization of the Cellular Immune Response Generated by Immunization with a DNA Vaccine Containing a Trypanosoma cruzi Gene. Infect. Immun. 67: 3855-3863 [Abstract] [Full Text]  
  • Tanghe, A., Lefevre, P., Denis, O., D'Souza, S., Braibant, M., Lozes, E., Singh, M., Montgomery, D., Content, J., Huygen, K. (1999). Immunogenicity and Protective Efficacy of Tuberculosis DNA Vaccines Encoding Putative Phosphate Transport Receptors. J. Immunol. 162: 1113-1119 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»