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Infect Immun, April 1998, p. 1748-1751, Vol. 66, No. 4
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Adherence to and Penetration of Human Intestinal Caco-2 Epithelial Cell Monolayers by Pseudomonas aeruginosa

Yoichi Hirakata,^1,* Kohichi Izumikawa,¹ Toshiyuki Yamaguchi,¹ Shizunobu Igimi,² Nobuhiko Furuya,³ Shigefumi Maesaki,⁴ Kazunori Tomono,⁴ Yasuaki Yamada,¹ Shigeru Kohno,⁴ Keizo Yamaguchi,³ and Shimeru Kamihira¹

Department of Laboratory Medicine¹ and Second Department of Internal Medicine,⁴ Nagasaki University School of Medicine, Nagasaki 852, Department of Biomedical Food Research, National Institute of Infectious Diseases, Tokyo 162,² and Department of Microbiology, Toho University School of Medicine, Tokyo 143,³ Japan

Received 10 November 1997/Returned for modification 9 December 1997/Accepted 31 December 1997

Clinical isolates of Pseudomonas aeruginosa from blood adhered to and penetrated intestinal Caco-2 cell monolayers to a greater degree than did isolates from sputum, with a concomitant drastic decrease in transepithelial electrical resistance. PAO-PR1, an avirulent exotoxin A mutant of PAO1, did not cause a decrease in the resistance. The Caco-2 monolayer system may be useful for the evaluation of certain P. aeruginosa virulence factor activities.

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^* Corresponding author. Mailing address: Department of Laboratory Medicine, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki 852, Japan. Phone: 81 (95) 849-7418. Fax: 81 (95) 849-7257. E-mail: hirakata{at}net.nagasaki-u.ac.jp.

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