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Infect Immun, April 1998, p. 1764-1767, Vol. 66, No. 4
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Bordetella pertussis Filamentous Hemagglutinin Enhances the Immunogenicity of Liposome-Delivered Antigen Administered Intranasally

Odile Poulain-Godefroy,1,* Nathalie Mielcarek,1,dagger Nathalie Ivanoff,1,Dagger Franck Remoué,1 Anne-Marie Schacht,1 Nigel Phillips,2 Camille Locht,3 André Capron,1 and Gilles Riveau1

INSERM U1671 and INSERM U477,3 Institut Pasteur de Lille, F-59019 Lille Cedex, France, and Faculté de Pharmacie, Université de Montréal, Montréal, Québec, Canada2

Received 5 September 1997/Returned for modification 24 November 1997/Accepted 20 January 1998

In an attempt to increase the immunogenicity of mucosally delivered antigens, we incorporated the Bordetella pertussis filamentous hemagglutinin (FHA) adhesin into liposomes containing the glutathione S-transferase of Schistosoma mansoni (Sm28GST) as a model antigen. Outbred mice immunized twice intranasally with liposomes containing a constant suboptimal dose of Sm28GST and increasing doses of FHA produced anti-Sm28GST antibodies in a FHA dose-dependent manner. The addition of 3 µg of FHA to the liposomes induced more than 10-fold-higher anti-Sm28GST antibody titers, compared to those induced by liposomes without FHA. The presence of FHA did not alter the nature of the humoral immune response, and the sera contained anti-Sm28GST immunoglobulin G1 (IgG1), IgG2a, and IgG2b. However, anti-Sm28GST IgA was only detected when at least 3 µg of FHA was added to the preparation. These results show a promising potential for FHA to enhance the immunogenicity of mucosally administered antigens incorporated into liposomes.

* Corresponding author. Mailing address: INSERM U167, Institut Pasteur de Lille, 1, rue du Professeur Calmette, BP 245, F-59019 Lille Cedex, France. Phone: (33) 3 20 87 77 81. Fax: (33) 3 20 87 78 88. E-mail: odile.poulain{at}pasteur-lille.fr.

dagger Present address: University of Göteborg, Dept. Medical Microbiology, 41346 Göteborg, Sweden.

Dagger Present address: Merck Sharp & Dohme Research Laboratories, 69367 Lyon Cedex 07, France.




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