This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wysong, D. R.
Right arrow Articles by Diamond, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wysong, D. R.
Right arrow Articles by Diamond, R. D.

 Previous Article  |  Next Article 

Infect Immun, May 1998, p. 1953-1961, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cloning and Sequencing of a Candida albicans Catalase Gene and Effects of Disruption of This Genedagger

Deborah R. Wysong,1,2 Laurent Christin,1 Alan M. Sugar,1 Phillips W. Robbins,2 and Richard D. Diamond1,*

Evans Memorial Department of Clinical Research and the Department of Medicine, Section of Infectious Diseases, Boston Medical Center, Boston, Massachusetts 02118,1 and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 021392

Received 19 June 1997/Returned for modification 5 August 1997/Accepted 25 February 1998

Catalase plays a key role as an antioxidant, protecting aerobic organisms from the toxic effects of hydrogen peroxide, and in some cases has been postulated to be a virulence factor. To help elucidate the function of catalase in Candida albicans, a single C. albicans-derived catalase gene, designated CAT1, was isolated and cloned. Degenerate PCR primers based on highly conserved areas of other fungal catalase genes were used to amplify a 411-bp product from genomic DNA of C. albicans ATCC 10261. By using this product as a probe, catalase clones were isolated from genomic libraries of C. albicans. Nucleotide sequence analysis revealed an open reading frame encoding a protein of 487 amino acid residues. Construction of a CAT1-deficient mutant was achieved by using the Ura-blaster technique for sequential disruption of multiple alleles by integrative transformation using URA3 as a selectable marker. Resulting mutants exhibited normal morphology and comparable growth rates of both yeast and mycelial forms. Enzymatic analysis revealed an abundance of catalase in the wild-type strain but decreasing catalase activity in heterozygous mutants and no detectable catalase in a homozygous null mutant. In vitro assays showed the mutant strains to be more sensitive to damage by both neutrophils and concentrations of exogenous peroxide that were sublethal for the parental strain. Compared to the parental strain, the homozygous null mutant strain was far less virulent for mice in an intravenous infection model of disseminated candidiasis. Definitive linkage of CAT1 with virulence would require restoration of activity by reintroduction of the gene into mutants. However, initial results in mice, taken together with the enhanced susceptibility of catalase-deficient hyphae to damage by human neutrophils, suggest that catalase may enhance the pathogenicity of C. albicans.

* Corresponding author. Mailing address: Section of Infectious Diseases, Rm. E-336, Boston Medical Center, 88 East Newton St., Boston, MA 02118. Phone: (617) 638-7909. Fax: (617) 638-8070. E-mail: rdiamond{at}med-med1.bu.edu.

dagger Publication 011 from the Collaborative Medical Mycology Research Program.

Infect Immun, May 1998, p. 1953-1961, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Bambach, A., Fernandes, M. P., Ghosh, A., Kruppa, M., Alex, D., Li, D., Fonzi, W. A., Chauhan, N., Sun, N., Agrellos, O. A., Vercesi, A. E., Rolfes, R. J., Calderone, R. (2009). Goa1p of Candida albicans Localizes to the Mitochondria during Stress and Is Required for Mitochondrial Function and Virulence. Eukaryot Cell 8: 1706-1720 [Abstract] [Full Text]  
  • Shary, S., Kapich, A. N., Panisko, E. A., Magnuson, J. K., Cullen, D., Hammel, K. E. (2008). Differential Expression in Phanerochaete chrysosporium of Membrane-Associated Proteins Relevant to Lignin Degradation. Appl. Environ. Microbiol. 74: 7252-7257 [Abstract] [Full Text]  
  • Ramsdale, M., Selway, L., Stead, D., Walker, J., Yin, Z., Nicholls, S. M., Crowe, J., Sheils, E. M., Brown, A. J.P. (2008). MNL1 Regulates Weak Acid-induced Stress Responses of the Fungal Pathogen Candida albicans. Mol. Biol. Cell 19: 4393-4403 [Abstract] [Full Text]  
  • Cuellar-Cruz, M., Briones-Martin-del-Campo, M., Canas-Villamar, I., Montalvo-Arredondo, J., Riego-Ruiz, L., Castano, I., De Las Penas, A. (2008). High Resistance to Oxidative Stress in the Fungal Pathogen Candida glabrata Is Mediated by a Single Catalase, Cta1p, and Is Controlled by the Transcription Factors Yap1p, Skn7p, Msn2p, and Msn4p. Eukaryot Cell 7: 814-825 [Abstract] [Full Text]  
  • Enjalbert, B., MacCallum, D. M., Odds, F. C., Brown, A. J. P. (2007). Niche-Specific Activation of the Oxidative Stress Response by the Pathogenic Fungus Candida albicans. Infect. Immun. 75: 2143-2151 [Abstract] [Full Text]  
  • Giles, S. S., Stajich, J. E., Nichols, C., Gerrald, Q. D., Alspaugh, J. A., Dietrich, F., Perfect, J. R. (2006). The Cryptococcus neoformans Catalase Gene Family and Its Role in Antioxidant Defense.. Eukaryot Cell 5: 1447-1459 [Abstract] [Full Text]  
  • Enjalbert, B., Smith, D. A., Cornell, M. J., Alam, I., Nicholls, S., Brown, A. J.P., Quinn, J. (2006). Role of the Hog1 Stress-activated Protein Kinase in the Global Transcriptional Response to Stress in the Fungal Pathogen Candida albicans. Mol. Biol. Cell 17: 1018-1032 [Abstract] [Full Text]  
  • Westwater, C., Balish, E., Schofield, D. A. (2005). Candida albicans-Conditioned Medium Protects Yeast Cells from Oxidative Stress: a Possible Link between Quorum Sensing and Oxidative Stress Resistance. Eukaryot Cell 4: 1654-1661 [Abstract] [Full Text]  
  • Nittler, M. P., Hocking-Murray, D., Foo, C. K., Sil, A. (2005). Identification of Histoplasma capsulatum Transcripts Induced in Response to Reactive Nitrogen Species. Mol. Biol. Cell 16: 4792-4813 [Abstract] [Full Text]  
  • Liu, T. T., Lee, R. E. B., Barker, K. S., Lee, R. E., Wei, L., Homayouni, R., Rogers, P. D. (2005). Genome-Wide Expression Profiling of the Response to Azole, Polyene, Echinocandin, and Pyrimidine Antifungal Agents in Candida albicans. Antimicrob. Agents Chemother. 49: 2226-2236 [Abstract] [Full Text]  
  • Sigle, H.-C., Thewes, S., Niewerth, M., Korting, H. C., Schafer-Korting, M., Hube, B. (2005). Oxygen accessibility and iron levels are critical factors for the antifungal action of ciclopirox against Candida albicans. J Antimicrob Chemother 55: 663-673 [Abstract] [Full Text]  
  • Smith, D. A., Nicholls, S., Morgan, B. A., Brown, A. J.P., Quinn, J. (2004). A Conserved Stress-activated Protein Kinase Regulates a Core Stress Response in the Human Pathogen Candida albicans. Mol. Biol. Cell 15: 4179-4190 [Abstract] [Full Text]  
  • Missall, T. A., Lodge, J. K., McEwen, J. E. (2004). Mechanisms of Resistance to Oxidative and Nitrosative Stress: Implications for Fungal Survival in Mammalian Hosts. Eukaryot Cell 3: 835-846 [Full Text]  
  • Brand, A., MacCallum, D. M., Brown, A. J. P., Gow, N. A. R., Odds, F. C. (2004). Ectopic Expression of URA3 Can Influence the Virulence Phenotypes and Proteome of Candida albicans but Can Be Overcome by Targeted Reintegration of URA3 at the RPS10 Locus. Eukaryot Cell 3: 900-909 [Abstract] [Full Text]  
  • Sanchez, A. A., Johnston, D. A., Myers, C., Edwards, J. E. Jr., Mitchell, A. P., Filler, S. G. (2004). Relationship between Candida albicans Virulence during Experimental Hematogenously Disseminated Infection and Endothelial Cell Damage In Vitro. Infect. Immun. 72: 598-601 [Abstract] [Full Text]  
  • Gil-Lamaignere, C., Roilides, E., Lyman, C. A., Simitsopoulou, M., Stergiopoulou, T., Maloukou, A., Walsh, T. J. (2003). Human Phagocytic Cell Responses to Scedosporium apiospermum (Pseudallescheria boydii): Variable Susceptibility to Oxidative Injury. Infect. Immun. 71: 6472-6478 [Abstract] [Full Text]  
  • Niewerth, M., Kunze, D., Seibold, M., Schaller, M., Korting, H. C., Hube, B. (2003). Ciclopirox Olamine Treatment Affects the Expression Pattern of Candida albicans Genes Encoding Virulence Factors, Iron Metabolism Proteins, and Drug Resistance Factors. Antimicrob. Agents Chemother. 47: 1805-1817 [Abstract] [Full Text]  
  • Moye-Rowley, W. S. (2003). Regulation of the Transcriptional Response to Oxidative Stress in Fungi: Similarities and Differences. Eukaryot Cell 2: 381-389 [Full Text]  
  • Alonso-Monge, R., Navarro-Garcia, F., Roman, E., Negredo, A. I., Eisman, B., Nombela, C., Pla, J. (2003). The Hog1 Mitogen-Activated Protein Kinase Is Essential in the Oxidative Stress Response and Chlamydospore Formation in Candidaalbicans. Eukaryot Cell 2: 351-361 [Abstract] [Full Text]  
  • Frankenberg, L., Brugna, M., Hederstedt, L. (2002). Enterococcus faecalis Heme-Dependent Catalase. J. Bacteriol. 184: 6351-6356 [Abstract] [Full Text]  
  • Hwang, C.-S., Rhie, G.-e., Oh, J.-H., Huh, W.-K., Yim, H.-S., Kang, S.-O. (2002). Copper- and zinc-containing superoxide dismutase (Cu/ZnSOD) is required for the protection of Candida albicans against oxidative stresses and the expression of its full virulence. Microbiology 148: 3705-3713 [Abstract] [Full Text]  
  • Huh, W.-K., Kim, S.-T., Kim, H., Jeong, G., Kang, S.-O. (2001). Deficiency of D-Erythroascorbic Acid Attenuates Hyphal Growth and Virulence of Candida albicans. Infect. Immun. 69: 3939-3946 [Abstract] [Full Text]  
  • Kawasaki, L., Aguirre, J. (2001). Multiple Catalase Genes Are Differentially Regulated in Aspergillus nidulans. J. Bacteriol. 183: 1434-1440 [Abstract] [Full Text]  
  • Odds, F. C., Van Nuffel, L., Gow, N. A. R. (2000). Survival in experimental Candida albicans infections depends on inoculum growth conditions as well as animal host. Microbiology 146: 1881-1889 [Abstract] [Full Text]  
  • Ghannoum, M. A. (2000). Potential Role of Phospholipases in Virulence and Fungal Pathogenesis. Clin. Microbiol. Rev. 13: 122-143 [Abstract] [Full Text]  
  • Leidich, S. D., Ibrahim, A. S., Fu, Y., Koul, A., Jessup, C., Vitullo, J., Fonzi, W., Mirbod, F., Nakashima, S., Nozawa, Y., Ghannoum, M. A. (1998). Cloning and Disruption of caPLB1, a Phospholipase B Gene Involved in the Pathogenicity of Candida albicans. J. Biol. Chem. 273: 26078-26086 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»