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Infect Immun, May 1998, p. 2099-2106, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification of Salmonella typhimurium Genes Required for Colonization of the Chicken Alimentary Tract and for Virulence in Newly Hatched Chicks

Arthur K. Turner,dagger Margaret A. Lovell, Scott D. Hulme, Li Zhang-Barber,Dagger and Paul A. Barrow*

Institute for Animal Health, Compton, Near Newbury, Berkshire RG20 7NN, United Kingdom

Received 17 November 1997/Returned for modification 23 December 1997/Accepted 28 January 1998

From a collection of 2,800 Tn5-TC1 transposon mutants of Salmonella typhimurium F98, 18 that showed reduced intestinal colonization of 3-week-old chicks were identified. The sites of transposon insertion were determined for most of the mutants and included insertions in the lipopolysaccharide biosynthesis genes rfaK, rfaY, rfbK, and rfbB and the genes dksA, clpB, hupA, and sipC. In addition, identification was made of an insertion into a novel gene that encodes a protein showing similarity to the IIC component of the mannose class of phosphoenolpyruvate-carbohydrate phosphotransferase systems, which we putatively called ptsC. Transduction of most of the transposon mutations to a fresh S. typhimurium F98 genetic background and construction of defined mutations in the rfbK, dksA, hupA, sipC, and ptsC genes of S. typhimurium F98 supported the role in colonization of all but the pts locus. The virulence of the rfbK, dksA, hupA, sipC, and ptsC defined mutants and clpB and rfaY transductants in 1-day-old chicks was tested. All but the ptsC and rfaY mutants were attenuated for virulence. A number of other phenotypes associated with some of the mutations are described.

* Corresponding author. Mailing address: Institute for Animal Health, Near Newbury, Berkshire RG20 7NN, United Kingdom. Phone: 44-1635-578411. Fax: 44-1635-577263. E-mail: paul.barrow{at}bbsrc.ac.uk.

dagger Present address: Peptide Therapeutics, Cambridge CB4 4WG, United Kingdom.

Dagger Present address: Biochemistry Department, Imperial College of Science Technology and Medicine, London SW7 2AZ, United Kingdom.

Infect Immun, May 1998, p. 2099-2106, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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