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Infect Immun, May 1998, p. 2337-2341, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Construction and Characterization of an Isogenic slt-ii Deletion Mutant of Enterohemorrhagic Escherichia coli

Florian Gunzer,1,dagger Ursula Bohn,1 Sibylle Fuchs,2 Inge Mühldorfer,2 Jörg Hacker,2 Saul Tzipori,1 and Arthur Donohue-Rolfe1,*

Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536,1 and Institut für Molekulare Infektionsbiologie der Universität Würzburg, 97070 Würzburg, Germany2

Received 8 September 1997/Returned for modification 24 November 1997/Accepted 6 February 1998

Enterohemorrhagic Escherichia coli (EHEC) produces Shiga-like toxins (SLT), potent protein synthesis inhibitors. To further dissect the role of SLT-II in the course of disease, we have constructed E. coli TUV86-2, an isogenic SLT-II-negative mutant of EHEC strain 86-24. The slt-ii gene was inactivated by suicide vector mutagenesis. We also isolated derivatives of strain 86-24 that were cured of the phage carrying the toxin genes.

* Corresponding author. Mailing address: Tufts University, School of Veterinary Medicine, 200 Westboro Rd., North Grafton, MA 01536. Phone: (508) 839-7919. Fax: (508) 839-7977. E-mail: adonohue_stp{at}opal.tufts.edu.

dagger Present address: Institute for Medical Microbiology, Hannover Medical School, D-30625 Hannover, Germany.

Infect Immun, May 1998, p. 2337-2341, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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