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Infect Immun, May 1998, p. 2387-2392, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Virulent Brucella abortus Prevents Lysosome Fusion and Is Distributed within Autophagosome-Like Compartments

Javier Pizarro-Cerdá,1 Edgardo Moreno,1 Veronique Sanguedolce,2 Jean-Louis Mege,2 and Jean-Pierre Gorvel1,*

Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, 13288 Marseille Cedex 9,1 and Centre National de Référence des Rickettsioses, Centre Hospitalier Universitaire la Timone, 13385 Marseille,2 France

Received 3 November 1997/Returned for modification 26 January 1998/Accepted 6 February 1998

Virulent and attenuated Brucella abortus strains attach to and penetrate nonprofessional phagocytic HeLa cells. Compared to pathogenic Brucella, the attenuated strain 19 hardly replicates within cells. The majority of the strain 19 bacteria colocalized with the lysosome marker cathepsin D, suggesting that Brucella-containing phagosomes had fused with lysosomes, in which they may have degraded. The virulent bacteria prevented lysosome-phagosome fusion and were found distributed in the perinuclear region within compartments resembling autophagosomes.

* Corresponding author. Mailing address: Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, Case 906, 13288 Marseille Cedex 9, France. Phone: 33-4-91-26-94-66. Fax: 33-4-91-26-94-30. E-mail: gorvel{at}ciml.univ-mrs.fr.

Infect Immun, May 1998, p. 2387-2392, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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