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Infect Immun, June 1998, p. 2529-2534, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

In Vitro Expression of Adhesion Receptors and Diapedesis by Polymorphonuclear Neutrophils during Experimentally Induced Streptococcus uberis Mastitis

Elke Smits,1 Christian Burvenich,1,* Albert J. Guidry,2 and Eddy Roets1

Milk Secretion and Mastitis Research Center, Faculty of Veterinary Medicine, University of Ghent, B9820 Merelbeke, Belgium,1 and Immunology and Disease Resistance Laboratory, Livestock and Poultry Sciences Institute, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 207052

Received 23 October 1997/Returned for modification 12 January 1998/Accepted 5 March 1998

The expression of adhesion receptors and diapedesis by polymorphonuclear neutrophils (PMN) were studied before and during experimentally induced Streptococcus uberis mastitis. Both quarters of the left half of the udders of five midlactation cows were inoculated with a suspension containing approximately 500 CFU of S. uberis 0140J. Clinical signs of an inflammatory reaction and leukocyte influx were observed 24 h after challenge. The expression of CD11b/CD18 adhesion receptors, determined by flow cytometry, was upregulated 24 h after challenge. A confluent monolayer of bovine secretory mammary epithelial cells on collagen-coated inserts was used to study PMN diapedesis. Bovine C5a was used as the chemoattractant. An 80% decrease in PMN diapedesis was observed 24 h after challenge. The decrease in diapedesis continued for 3 weeks after challenge.

* Corresponding author. Mailing address: Milk Secretion and Mastitis Research Center, Faculty of Veterinary Medicine, University of Ghent, Salisburylaan 133, B9820 Merelbeke, Belgium. Phone: 32 (0) 92647321. Fax: 32 (0) 92647499. E-mail: christian.burvenich{at}rug.ac.be.

Infect Immun, June 1998, p. 2529-2534, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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