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Infect Immun, June 1998, p. 2854-2858, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Immunodominant Brugia malayi Paramyosin as a Marker of Current Infection with Wuchereria bancrofti Adult Worms

Sandra Langy,1 Catherine Plichart,1 Patrick Luquiaud,1 Steven A. Williams,2,3 and Luc Nicolas1,*

Institut Territorial de Recherches Médicales Louis Malardé, Papeete, Tahiti, French Polynesia1; Department of Biological Sciences, Clark Science Center, Smith College, Northampton, Massachusetts 010632; and Molecular and Cellular Biology, University of Massachusetts, Amherst, Massachusetts 010033

Received 15 December 1997/Returned for modification 30 January 1998/Accepted 18 March 1998

The full-length cDNA sequence encoding Brugia malayi L3 paramyosin has been isolated by immunoscreening a cDNA library with a mouse antiserum raised against Wuchereria bancrofti L3 infective larvae. A recombinant truncated form of paramyosin was expressed as a glutathione S-transferase fusion protein and used to evaluate humoral responses of adults from a W. bancrofti-endemic area in French Polynesia according to their parasitological status. Immunoglobulin G4 (IgG4) preferentially bound to paramyosin in W. bancrofti-parasitized individuals, in contrast to unparasitized individuals, who harbored neither microfilaria nor Og4C3 adult worm circulating antigen. Reduction of the anti-paramyosin IgG4 titer following combined chemotherapy with diethylcarbamazine and ivermectin was significantly correlated with a reduction in the adult worm burden. This indicates that the presence of paramyosin-reactive IgG4 is associated with the presence of parasites and that reduction can be used as an immunological marker for W. bancrofti clearance.

* Corresponding author. Present address: Unité d'Immunophysiologie Cellulaire, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France. Phone: (33) 1 45 68 86 69; Fax: (33) 1 40 61 31 69. E-mail: lnicolas{at}pasteur.fr.

Infect Immun, June 1998, p. 2854-2858, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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