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Infect Immun, June 1998, p. 2938-2942, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Goldfish, Carassius auratus, a Novel Animal Model for the Study of Mycobacterium marinum Pathogenesis

Adel M. Talaat,1 Renate Reimschuessel,2 Steven S. Wasserman,1 and Michele Trucksis1,3,*

Medical Service, Veterans' Affairs Medical Center,3 and Center for Vaccine Development, Division of Geographic Medicine, Department of Medicine,1 and Department of Pathology,2 University of Maryland School of Medicine, Baltimore, Maryland

Received 1 October 1997/Returned for modification 8 January 1998/Accepted 13 March 1998

We have developed an animal model for studying mycobacterial pathogenesis using Mycobacterium marinum and the goldfish, Carassius auratus. Goldfish are injected intraperitoneally with doses between 102 and 109 CFU of M. marinum organisms. Depending on the dose of M. marinum organisms administered, an acute or chronic disease is produced. The acute disease is characterized by systemic mycobacterial infection, severe peritonitis, tissue necrosis, and a short median survival time. The chronic disease is characterized by granuloma formation in all organs and survival of animals to the end point of the experiment (56 days). Colony counts in organ homogenates showed recovery of mycobacteria from a high percentage of inoculated animals. We believe this well-characterized animal model will be useful for studying mycobacterial pathogenesis.

* Corresponding author. Mailing address: Center for Vaccine Development, Division of Geographic Medicine, University of Maryland School of Medicine, 685 W. Baltimore St., Baltimore, MD 21201. Phone: (410) 706-5328. Fax: (410) 706-6205. E-mail: mtrucksi{at}umppa1.ab.umd.edu.

Infect Immun, June 1998, p. 2938-2942, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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