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Infect Immun, June 1998, p. 2996-2998, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Assimilation of Xylose, Mannose, and Mannitol for Synthesis of Glucuronoxylomannan of Cryptococcus neoformans Determined by 13C Nuclear Magnetic Resonance Spectroscopy

Robert Cherniak,* Eric B. O'Neill, and Shuqun Shengdagger

Laboratory for Biological and Chemical Sciences, Department of Chemistry, Georgia State University, Atlanta, Georgia

Received 10 February 1998/Returned for modification 13 March 1998/Accepted 26 March 1998

Cryptococcus neoformans NIH 409 was cultured in a defined medium containing D-[1-13C]xylose (Xyl), D-[1-13C]mannose (Man), or D-[1-13C]mannitol as the sole carbon source. The distribution of 13C in the Man, Xyl, glucuronic acid (GlcA), and O-acetyl constituents of native and de-O-acetylated glucuronoxylomannan (GXM) was determined by one-dimensional 13C nuclear magnetic resonance spectroscopy. The carbon chain of Man was incorporated intact into GXM since 13C was observed only in carbon 1 of Man, GlcA, and Xyl. The carbon chain of mannitol was incorporated intact into GXM since 13C was observed only in carbons 1 and 6. This was expected since mannitol has an axis of symmetry. The carbon chain of Xyl was identified only in carbons 1 and 3 of Man, GlcA, and Xyl. This pattern of labeling is consistent with the assimilation of Xyl through the pentophosphate pathway.

* Corresponding author. Mailing address: Department of Chemistry, Georgia State University, University Plaza, Atlanta, GA 30303. Phone: (404) 651-3868. Fax: (404) 651-1416. E-mail: cherniak{at}gsu.edu.

dagger Present address: Department of Chemistry, Rutgers University, Piscataway, N.J.

Infect Immun, June 1998, p. 2996-2998, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.




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