This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wong, K.-K.
Right arrow Articles by Saffer, J. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wong, K.-K.
Right arrow Articles by Saffer, J. D.

 Previous Article  |  Next Article 

Infect Immun, July 1998, p. 3365-3371, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification and Sequence Analysis of a 27-Kilobase Chromosomal Fragment Containing a Salmonella Pathogenicity Island Located at 92 Minutes on the Chromosome Map of Salmonella enterica Serovar Typhimurium LT2

Kwong-Kwok Wong,1,* Michael McClelland,2 Lisa C. Stillwell,1 Ellen C. Sisk,1,dagger Sarah J. Thurston,1 and Jeffrey D. Saffer1

Molecular Biosciences, Pacific Northwest National Laboratory, Richland, Washington 99352,1 and Sidney Kimmel Cancer Center, San Diego, California 921212

Received 13 February 1998/Returned for modification 8 April 1998/Accepted 30 April 1998

Using a genomic approach, we have identified a new Salmonella pathogenicity island, SPI-4, which is the fourth Salmonella pathogenicity island to be identified. SPI-4 was located at 92 min on the chromosome map and was flanked by the ssb and soxSR loci. The DNA sequence covering the entire SPI-4 and both boundaries was determined. The size of SPI-4 was about 25 kb and it contains 18 putative open reading frames (ORFs). Three of these ORFs encode proteins that have significant homology with proteins involved in toxin secretion. Another five ORFs encode proteins that have significant homology with hypothetical proteins from Synechocystis sp. strain PCC6803 or Acinetobacter calcoaceticus. The rest of the ORFs encode novel proteins, one of which has five membrane-spanning domains. SPI-4 is likely to carry a type I secretion system involved in toxin secretion. Furthermore, a previously identified locus (ims98), which is required for intramacrophage survival, was also mapped within the SPI-4 region. These findings suggested that SPI-4 is needed for intramacrophage survival.

* Corresponding author. Mailing address: Molecular Biosciences, P7-56, Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA 99352. Phone: (509) 376-5097. Fax: (509) 376-6767. E-mail: kk.wong{at}pnl.gov.

dagger Present address: Seattle Biomedical Research Institute, Seattle, WA 98109-1651.

Infect Immun, July 1998, p. 3365-3371, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Thomson, N. R., Clayton, D. J., Windhorst, D., Vernikos, G., Davidson, S., Churcher, C., Quail, M. A., Stevens, M., Jones, M. A., Watson, M., Barron, A., Layton, A., Pickard, D., Kingsley, R. A., Bignell, A., Clark, L., Harris, B., Ormond, D., Abdellah, Z., Brooks, K., Cherevach, I., Chillingworth, T., Woodward, J., Norberczak, H., Lord, A., Arrowsmith, C., Jagels, K., Moule, S., Mungall, K., Sanders, M., Whitehead, S., Chabalgoity, J. A., Maskell, D., Humphrey, T., Roberts, M., Barrow, P. A., Dougan, G., Parkhill, J. (2008). Comparative genome analysis of Salmonella Enteritidis PT4 and Salmonella Gallinarum 287/91 provides insights into evolutionary and host adaptation pathways. Genome Res 18: 1624-1637 [Abstract] [Full Text]  
  • Main-Hester, K. L., Colpitts, K. M., Thomas, G. A., Fang, F. C., Libby, S. J. (2008). Coordinate Regulation of Salmonella Pathogenicity Island 1 (SPI1) and SPI4 in Salmonella enterica Serovar Typhimurium. Infect. Immun. 76: 1024-1035 [Abstract] [Full Text]  
  • Garcia-Calderon, C. B., Casadesus, J., Ramos-Morales, F. (2007). Rcs and PhoPQ Regulatory Overlap in the Control of Salmonella enterica Virulence. J. Bacteriol. 189: 6635-6644 [Abstract] [Full Text]  
  • Thijs, I. M. V., De Keersmaecker, S. C. J., Fadda, A., Engelen, K., Zhao, H., McClelland, M., Marchal, K., Vanderleyden, J. (2007). Delineation of the Salmonella enterica Serovar Typhimurium HilA Regulon through Genome-Wide Location and Transcript Analysis. J. Bacteriol. 189: 4587-4596 [Abstract] [Full Text]  
  • Morgan, E., Bowen, A. J., Carnell, S. C., Wallis, T. S., Stevens, M. P. (2007). SiiE Is Secreted by the Salmonella enterica Serovar Typhimurium Pathogenicity Island 4-Encoded Secretion System and Contributes to Intestinal Colonization in Cattle. Infect. Immun. 75: 1524-1533 [Abstract] [Full Text]  
  • De Keersmaecker, S. C. J., Marchal, K., Verhoeven, T. L. A., Engelen, K., Vanderleyden, J., Detweiler, C. S. (2005). Microarray Analysis and Motif Detection Reveal New Targets of the Salmonella enterica Serovar Typhimurium HilA Regulatory Protein, Including hilA Itself. J. Bacteriol. 187: 4381-4391 [Abstract] [Full Text]  
  • Schmidt, H., Hensel, M. (2004). Pathogenicity Islands in Bacterial Pathogenesis. Clin. Microbiol. Rev. 17: 14-56 [Abstract] [Full Text]  
  • Ellermeier, C. D., Slauch, J. M. (2003). RtsA and RtsB Coordinately Regulate Expression of the Invasion and Flagellar Genes in Salmonella enterica Serovar Typhimurium. J. Bacteriol. 185: 5096-5108 [Abstract] [Full Text]  
  • Amavisit, P., Lightfoot, D., Browning, G. F., Markham, P. F. (2003). Variation between Pathogenic Serovars within Salmonella Pathogenicity Islands. J. Bacteriol. 185: 3624-3635 [Abstract] [Full Text]  
  • Hansen-Wester, I., Hensel, M. (2002). Genome-Based Identification of Chromosomal Regions Specific for Salmonella spp.. Infect. Immun. 70: 2351-2360 [Abstract] [Full Text]  
  • Santos, R. L., Tsolis, R. M., Baumler, A. J., Smith, R. III, Adams, L. G. (2001). Salmonella enterica Serovar Typhimurium Induces Cell Death in Bovine Monocyte-Derived Macrophages by Early sipB-Dependent and Delayed sipB-Independent Mechanisms. Infect. Immun. 69: 2293-2301 [Abstract] [Full Text]  
  • WINSTANLEY, C., HART, C. A. (2001). Type III secretion systems and pathogenicity islands. J Med Microbiol 50: 116-126 [Abstract] [Full Text]  
  • McClelland, M., Florea, L., Sanderson, K., Clifton, S. W., Parkhill, J., Churcher, C., Dougan, G., Wilson, R. K., Miller, W. (2000). Comparison of the Escherichia coli K-12 genome with sampled genomes of a Klebsiella pneumoniae and three Salmonella enterica serovars, Typhimurium, Typhi and Paratyphi. Nucleic Acids Res 28: 4974-4986 [Abstract] [Full Text]  
  • Altier, C., Suyemoto, M., Lawhon, S. D. (2000). Regulation of Salmonella enterica Serovar Typhimurium Invasion Genes by csrA. Infect. Immun. 68: 6790-6797 [Abstract] [Full Text]  
  • HART, C.A., BEECHING, N.J., DUERDEN, B.I. (2000). Infections in AIDS: Proceedings of the Sixth Liverpool Tropical School Bayer Symposium on Microbial Diseases held on 6 February 1999. J Med Microbiol 49: 947-967 [Full Text]  
  • Julio, S. M., Heithoff, D. M., Mahan, M. J. (2000). ssrA (tmRNA) Plays a Role in Salmonella enterica Serovar Typhimurium Pathogenesis. J. Bacteriol. 182: 1558-1563 [Abstract] [Full Text]  
  • Watson, P. R., Paulin, S. M., Bland, A. P., Libby, S. J., Jones, P. W., Wallis, T. S. (1999). Differential Regulation of Enteric and Systemic Salmonellosis by slyA. Infect. Immun. 67: 4950-4954 [Abstract] [Full Text]  
  • Fang, F. C., DeGroote, M. A., Foster, J. W., Baumler, A. J., Ochsner, U., Testerman, T., Bearson, S., Giard, J.-C., Xu, Y., Campbell, G., Laessig, T. (1999). Virulent Salmonella typhimurium has two periplasmic Cu, Zn-superoxide dismutases. Proc. Natl. Acad. Sci. USA 96: 7502-7507 [Abstract] [Full Text]  
  • Blanc-Potard, A.-B., Solomon, F., Kayser, J., Groisman, E. A. (1999). The SPI-3 Pathogenicity Island of Salmonella enterica. J. Bacteriol. 181: 998-1004 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»