This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jonquières, R.
Right arrow Articles by Cossart, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jonquières, R.
Right arrow Articles by Cossart, P.

 Previous Article  |  Next Article 

Infect Immun, July 1998, p. 3420-3422, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The inlA Gene of Listeria monocytogenes LO28 Harbors a Nonsense Mutation Resulting in Release of Internalin

Renaud Jonquières, Hélène Bierne, Jérôme Mengaud, and Pascale Cossart*

Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris, France

Received 4 February 1998/Returned for modification 6 March 1998/Accepted 2 April 1998

Internalin is a surface protein that mediates entry of Listeria monocytogenes EGD into epithelial cells expressing the cell adhesion molecule human E-cadherin or its chicken homolog, L-CAM, which act as receptors for internalin. After observing that entry of L. monocytogenes LO28 into S180 fibroblasts, in contrast to that of EGD, did not increase after transfection with L-CAM, we examined both the expression and the structure of internalin in strain LO28. We discovered a nonsense mutation in inlA which results in a truncated protein released in the culture medium. Mutations leading to release of internalin were also detected in clinical and food isolates. These results question the role of internalin as a virulence factor in murine listeriosis.

* Corresponding author. Mailing address: Unité des Interactions Bactéries-Cellules, Institut Pasteur, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France. Phone: 33 1 45 68 88 41. Fax: 33 1 45 68 87 06. E-mail: pcossart{at}pasteur.fr.

Infect Immun, July 1998, p. 3420-3422, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Bonazzi, M., Lecuit, M., Cossart, P. (2009). Listeria monocytogenes Internalin and E-cadherin: From Bench to Bedside. Cold Spring Harb. Perspect. Biol. 1: a003087-a003087 [Abstract] [Full Text]  
  • Ward, T. J., Ducey, T. F., Usgaard, T., Dunn, K. A., Bielawski, J. P. (2008). Multilocus Genotyping Assays for Single Nucleotide Polymorphism-Based Subtyping of Listeria monocytogenes Isolates. Appl. Environ. Microbiol. 74: 7629-7642 [Abstract] [Full Text]  
  • Van Stelten, A., Nightingale, K. K. (2008). Development and Implementation of a Multiplex Single-Nucleotide Polymorphism Genotyping Assay for Detection of Virulence-Attenuating Mutations in the Listeria monocytogenes Virulence-Associated Gene inlA. Appl. Environ. Microbiol. 74: 7365-7375 [Abstract] [Full Text]  
  • Nightingale, K. K., Ivy, R. A., Ho, A. J., Fortes, E. D., Njaa, B. L., Peters, R. M., Wiedmann, M. (2008). inlA Premature Stop Codons Are Common among Listeria monocytogenes Isolates from Foods and Yield Virulence-Attenuated Strains That Confer Protection against Fully Virulent Strains. Appl. Environ. Microbiol. 74: 6570-6583 [Abstract] [Full Text]  
  • Temoin, S., Roche, S. M., Grepinet, O., Fardini, Y., Velge, P. (2008). Multiple point mutations in virulence genes explain the low virulence of Listeria monocytogenes field strains. Microbiology 154: 939-948 [Abstract] [Full Text]  
  • Orsi, R. H., Ripoll, D. R., Yeung, M., Nightingale, K. K., Wiedmann, M. (2007). Recombination and positive selection contribute to evolution of Listeria monocytogenes inlA. Microbiology 153: 2666-2678 [Abstract] [Full Text]  
  • Felicio, M. T. S., Hogg, T., Gibbs, P., Teixeira, P., Wiedmann, M. (2007). Recurrent and Sporadic Listeria monocytogenes Contamination in Alheiras Represents Considerable Diversity, Including Virulence-Attenuated Isolates. Appl. Environ. Microbiol. 73: 3887-3895 [Abstract] [Full Text]  
  • Ducey, T. F., Page, B., Usgaard, T., Borucki, M. K., Pupedis, K., Ward, T. J. (2007). A Single-Nucleotide-Polymorphism-Based Multilocus Genotyping Assay for Subtyping Lineage I Isolates of Listeria monocytogenes. Appl. Environ. Microbiol. 73: 133-147 [Abstract] [Full Text]  
  • Nightingale, K. K., Windham, K., Martin, K. E., Yeung, M., Wiedmann, M. (2005). Select Listeria monocytogenes Subtypes Commonly Found in Foods Carry Distinct Nonsense Mutations in inlA, Leading to Expression of Truncated and Secreted Internalin A, and Are Associated with a Reduced Invasion Phenotype for Human Intestinal Epithelial Cells. Appl. Environ. Microbiol. 71: 8764-8772 [Abstract] [Full Text]  
  • Nightingale, K. K., Windham, K., Wiedmann, M. (2005). Evolution and Molecular Phylogeny of Listeria monocytogenes Isolated from Human and Animal Listeriosis Cases and Foods. J. Bacteriol. 187: 5537-5551 [Abstract] [Full Text]  
  • Rousseaux, S., Olier, M., Lemaitre, J. P., Piveteau, P., Guzzo, J. (2004). Use of PCR-Restriction Fragment Length Polymorphism of inlA for Rapid Screening of Listeria monocytogenes Strains Deficient in the Ability To Invade Caco-2 Cells. Appl. Environ. Microbiol. 70: 2180-2185 [Abstract] [Full Text]  
  • Pilgrim, S., Kolb-Maurer, A., Gentschev, I., Goebel, W., Kuhn, M. (2003). Deletion of the Gene Encoding p60 in Listeria monocytogenes Leads to Abnormal Cell Division and Loss of Actin-Based Motility. Infect. Immun. 71: 3473-3484 [Abstract] [Full Text]  
  • Olier, M., Pierre, F., Rousseaux, S., Lemaitre, J.-P., Rousset, A., Piveteau, P., Guzzo, J. (2003). Expression of Truncated Internalin A Is Involved in Impaired Internalization of Some Listeria monocytogenes Isolates Carried Asymptomatically by Humans. Infect. Immun. 71: 1217-1224 [Abstract] [Full Text]  
  • Olier, M., Pierre, F., Lemaitre, J.-P., Divies, C., Rousset, A., Guzzo, J. (2002). Assessment of the pathogenic potential of two Listeria monocytogenes human faecal carriage isolates. Microbiology 148: 1855-1862 [Abstract] [Full Text]  
  • Garandeau, C., Reglier-Poupet, H., Dubail, I., Beretti, J.-L., Berche, P., Charbit, A. (2002). The Sortase SrtA of Listeria monocytogenes Is Involved in Processing of Internalin and in Virulence. Infect. Immun. 70: 1382-1390 [Abstract] [Full Text]  
  • Marino, M., Braun, L., Cossart, P., Ghosh, P. (2000). A framework for interpreting the leucine-rich repeats of the Listeria internalins. Proc. Natl. Acad. Sci. USA 97: 8784-8788 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»