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Infect Immun, July 1998, p. 3429-3432, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Attachment of Cryptosporidium parvum Sporozoites to Human Intestinal Epithelial Cells

Angela Joe,1,dagger Renaud Verdon,1,Dagger Saul Tzipori,2 Gerald T. Keusch,1 and Honorine D. Ward1,*

Division of Geographic Medicine and Infectious Diseases, New England Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02111,1 and Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 015362

Received 23 December 1997/Returned for modification 20 February 1998/Accepted 8 April 1998

An enzyme-linked immunosorbent assay-based attachment model using the human intestinal cell line Caco-2A was developed to study attachment of Cryptosporidium parvum sporozoites in vitro and to assess potential inhibitors of sporozoite binding. In this system, attachment was related to sporozoite dose, incubation time, and host cell differentiation status. Polyclonal antibodies to C. parvum as well as glycoprotein inhibitors of a sporozoite lectin reduced attachment. This model will be a valuable tool in elucidating specific molecules and mechanisms involved in sporozoite-host cell attachment.

* Corresponding author. Mailing address: New England Medical Center, Division of Geographic Medicine and Infectious Diseases, 750 Washington St., NEMC Box 041, Boston, MA 02111. Phone: (617) 636-7032. Fax: (617) 636-3216. E-mail: honorine.ward{at}es.nemc.org.

dagger Present address: Department of Medical Genetics and Microbiology, University of Toronto, Ontario, Canada M5S 3E2.

Dagger Present address: Reanimation Medicale, Maladies Infectieuses et Tropicales, Centre Hospitalier Universitaire Cote-de-Nacre, Caen 14033, France.

Infect Immun, July 1998, p. 3429-3432, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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