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Infect Immun, July 1998, p. 3454-3456, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Delayed-Type Hypersensitivity Responses to ESAT-6 and MPT64 from Mycobacterium tuberculosis in the Guinea Pig

Martin J. Elhay, Thomas Oettinger, and Peter Andersen*

Department of TB Immunology, Statens Serum Institut, Copenhagen, Denmark

Received 3 November 1997/Returned for modification 7 January 1998/Accepted 24 April 1998

Two antigens from Mycobacterium tuberculosis, ESAT-6 and MPT64, elicited delayed-type hypersensitivity (DTH) skin responses in outbred guinea pigs infected with M. tuberculosis by the aerosol and intravenous routes but not those sensitized with M. bovis BCG or M. avium. The DTH epitope of ESAT-6 was mapped to the C terminus. Nonresponders to the individual antigens were found, but all animals responded to a combination of ESAT-6 and MPT64 or their respective minimal target peptides. Correspondingly, these molecules could form the basis of a new skin test for tuberculosis.

* Corresponding author. Mailing address: Department of T.B. Immunology, Statens Serum Institut, Artillerivej 5, Copenhagen 2300 S, Denmark. Phone: (45) 32 68 34 62. Fax: (45) 3268 30 35. E-mail: tbimm{at}ssi.dk.

Infect Immun, July 1998, p. 3454-3456, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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