This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Coconnier, M.-H.
Right arrow Articles by Servin, A. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Coconnier, M.-H.
Right arrow Articles by Servin, A. L.

 Previous Article  |  Next Article 

Infect Immun, August 1998, p. 3673-3681, Vol. 66, No. 8
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Listeria monocytogenes Stimulates Mucus Exocytosis in Cultured Human Polarized Mucosecreting Intestinal Cells through Action of Listeriolysin O

Marie-Hélène Coconnier,1 Elyess Dlissi,1 Myriam Robard,2 Christian L. Laboisse,2 Jean-Louis Gaillard,3 and Alain L. Servin1 *

CJF 94.07 INSERM, Pathogénie Cellulaire et Moléculaire des Microorganismes Entérovirulents, Faculté de Pharmacie Paris XI, F-92296 Châtenay-Malabry,1 CJF 94.04 INSERM, Fonctions Secrétoires des Epithélium Digestifs, Faculté de Médecine, F-44035 Nantes,2 and Unité 411 INSERM, Physiopathologie Moléculaire des Infections Microbiennes, Faculté Necker, F-75730 Paris,3 France

Received 4 March 1998/Returned for modification 21 April 1998/Accepted 12 May 1998

When the intracellular pathogen Listeria monocytogenes infects cultured human mucosecreting polarized HT29-MTX cells apically, it induces the stimulation of mucus exocytosis without cell entry. Using a set of isogenic mutants and purified listeriolysin O (LLO), we identified the L. monocytogenes thiol-activated exotoxin LLO as the agonist of mucus secretion. We demonstrated that the LLO-induced mucus exocytosis did not result from the LLO membrane-damaging activity. We found that LLO-induced mucus exocytosis is an event requiring the binding of LLO to a brush border-associated receptor and membrane oligomerization of the exotoxin. By a pharmacological approach, we demonstrated that no regulatory system or intracellular transducing signal known to be involved in control of mucin exocytosis was activated by LLO. Based on the present data, the stimulatory action of LLO on mucin exocytosis could be accounted for either by an unknown signaling system which remains to be determined or by direct action of LLO with the membrane vesicle components involved in the intracellular vesicular transport of mucins.

* Corresponding author. Mailing address: CJF 94.07 INSERM, Faculté de Pharmacie Paris XI, F-92296 Châtenay-Malabry, France. Phone and fax: 01.46.83.56.61. E-mail: alain.servin{at}cep.u-psud.fr.

Infect Immun, August 1998, p. 3673-3681, Vol. 66, No. 8
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Richter, J. F., Gitter, A. H., Gunzel, D., Weiss, S., Mohamed, W., Chakraborty, T., Fromm, M., Schulzke, J. D. (2009). Listeriolysin O affects barrier function and induces chloride secretion in HT-29/B6 colon epithelial cells. Am. J. Physiol. Gastrointest. Liver Physiol. 296: G1350-G1359 [Abstract] [Full Text]  
  • Lievin-Le Moal, V., Servin, A. L. (2006). The Front Line of Enteric Host Defense against Unwelcome Intrusion of Harmful Microorganisms: Mucins, Antimicrobial Peptides, and Microbiota. Clin. Microbiol. Rev. 19: 315-337 [Abstract] [Full Text]  
  • Cheng, L. W., Viala, J. P. M., Stuurman, N., Wiedemann, U., Vale, R. D., Portnoy, D. A. (2005). Use of RNA interference in Drosophila S2 cells to identify host pathways controlling compartmentalization of an intracellular pathogen. Proc. Natl. Acad. Sci. USA 102: 13646-13651 [Abstract] [Full Text]  
  • Aristoteli, L. P., Willcox, M. D. P. (2003). Mucin Degradation Mechanisms by Distinct Pseudomonas aeruginosa Isolates In Vitro. Infect. Immun. 71: 5565-5575 [Abstract] [Full Text]  
  • Vazquez-Boland, J. A., Kuhn, M., Berche, P., Chakraborty, T., Dominguez-Bernal, G., Goebel, W., Gonzalez-Zorn, B., Wehland, J., Kreft, J. (2001). Listeria Pathogenesis and Molecular Virulence Determinants. Clin. Microbiol. Rev. 14: 584-640 [Abstract] [Full Text]  
  • Deplancke, B., Gaskins, H R. (2001). Microbial modulation of innate defense: goblet cells and the intestinal mucus layer. Am. J. Clin. Nutr. 73 : 1131S-1141S [Abstract] [Full Text]  
  • Edelson, B. T., Cossart, P., Unanue, E. R. (1999). Cutting Edge: Paradigm Revisited: Antibody Provides Resistance to Listeria Infection. J. Immunol. 163: 4087-4090 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»