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Infect Immun, August 1998, p. 3783-3787, Vol. 66, No. 8
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Only Viable Parasites Are Detected by PCR following Clearance of Rodent Malarial Infections by Drug Treatment or Immune Responses

William Jarra1 and Georges Snounou2 *

Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA,1 and Department of Infection and Tropical Medicine, Imperial College School of Medicine, Lister Unit, Northwick Park Hospital, Harrow, Middlesex HA1 3UJ,2 United Kingdom

Received 3 March 1998/Returned for modification 24 April 1998/Accepted 11 May 1998

Detection and analysis of pathogens by PCR plays an important role in infectious disease research. The value of these studies would be diminished if nuclear material from dead parasites were found to remain in circulation for extended periods and thus result in positive amplification. This possibility was tested in experimental rodent malaria infections. Blood samples were obtained from infected mice during and following drug or immune clearance of Plasmodium chabaudi chabaudi parasitemias. Detection of parasite DNA by a sensitive Plasmodium-specific PCR amplification assay was associated with the presence of viable parasites, as detected by subinoculation. No parasite DNA could be detected by PCR 48 h after the injection of killed parasites into mice. Nuclear material from parasites removed by drug or immune responses is rapidly cleared from the circulation and does not contribute significantly to amplification. Thus, results from PCR analysis of malaria-infected blood accurately reflect the presence of live parasites.

* Corresponding author. Mailing address: Department of Infection and Tropical Medicine, Imperial College School of Medicine, Lister Unit, Northwick Park Hospital, Harrow, Middlesex HA1 3UJ, United Kingdom. Phone: (44) (181) 869 3507. Fax: (44) (181) 869 3504. E-mail: g.snounou{at}ic.ac.uk.

Infect Immun, August 1998, p. 3783-3787, Vol. 66, No. 8
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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