This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Comerci, D. J.
Right arrow Articles by Ugalde, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Comerci, D. J.
Right arrow Articles by Ugalde, R. A.

 Previous Article  |  Next Article 

Infect Immun, August 1998, p. 3862-3866, Vol. 66, No. 8
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Vector Development for the Expression of Foreign Proteins in the Vaccine Strain Brucella abortus S19

Diego J. Comerci,1 2 Guido D. Pollevick,1 Ana M. Vigliocco,2 Alberto C. C. Frasch,1 and Rodolfo A. Ugalde1 *

Instituto de Investigaciones Biotecnologicas, Universidad Nacional de General San Martin, INTI, San Martin, Provincia de Buenos Aires,1 and Grupo de Investigaciones Pecuarias, Centro Atomico Ezeiza, Comision Nacional de Energia Atomica, Buenos Aires,2 Argentina

Received 12 January 1998/Returned for modification 18 February 1998/Accepted 22 April 1998

A vector for the expression of foreign antigens in the vaccine strain Brucella abortus S19 was developed by using a DNA fragment containing the regulatory sequences and the signal peptide of the Brucella bcsp31 gene. This fragment was cloned in broad-host-range plasmid pBBR4MCS, resulting in plasmid pBEV. As a reporter protein, a repetitive antigen of Trypanosoma cruzi was used. The recombinant fusion protein is stably expressed and secreted into the Brucella periplasmic space, inducing a good antibody response against the T. cruzi antigen. The expression of the repetitive antigen in Brucella neither altered its growth pattern nor generated a toxic or lethal effect during experimental infection. The application of this strategy for the generation of live recombinant vaccines and the tagging of B. abortus S19 vaccine is discussed. This is the first time that a recombinant protein has been expressed in the periplasm of brucellae.

* Corresponding author. Mailing address: Instituto de Investigaciones Biotecnologicas, Universidad Nacional de General San Martin, INTI, Av. General Paz y Albarellos (Ed. 24), Casilla de Correo 30 (1650), San Martin, Provincia de Buenos Aires, Argentina. Phone: (54-1) 752-0021. Fax: (54-1) 752-9639. E-mail: rugalde{at}inti.gov.ar.

Infect Immun, August 1998, p. 3862-3866, Vol. 66, No. 8
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Spera, J. M., Ugalde, J. E., Mucci, J., Comerci, D. J., Ugalde, R. A. (2006). A B lymphocyte mitogen is a Brucella abortus virulence factor required for persistent infection. Proc. Natl. Acad. Sci. USA 103: 16514-16519 [Abstract] [Full Text]  
  • Roset, M. S., Ciocchini, A. E., Ugalde, R. A., Inon de Iannino, N. (2006). The Brucella abortus Cyclic {beta}-1,2-Glucan Virulence Factor Is Substituted with O-Ester-Linked Succinyl Residues.. J. Bacteriol. 188: 5003-5013 [Abstract] [Full Text]  
  • Comerci, D. J., Altabe, S., de Mendoza, D., Ugalde, R. A. (2006). Brucella abortus Synthesizes Phosphatidylcholine from Choline Provided by the Host.. J. Bacteriol. 188: 1929-1934 [Abstract] [Full Text]  
  • Roset, M. S., Ciocchini, A. E., Ugalde, R. A., Inon de Iannino, N. (2004). Molecular Cloning and Characterization of cgt, the Brucella abortus Cyclic {beta}-1,2-Glucan Transporter Gene, and Its Role in Virulence. Infect. Immun. 72: 2263-2271 [Abstract] [Full Text]  
  • Briones, G., Inon de Iannino, N., Roset, M., Vigliocco, A., Paulo, P. S., Ugalde, R. A. (2001). Brucella abortus Cyclic {beta}-1,2-Glucan Mutants Have Reduced Virulence in Mice and Are Defective in Intracellular Replication in HeLa Cells. Infect. Immun. 69: 4528-4535 [Abstract] [Full Text]  
  • Sieira, R., Comerci, D. J., Sánchez, D. O., Ugalde, R. A. (2000). A Homologue of an Operon Required for DNA Transfer in Agrobacterium Is Required in Brucella abortus for Virulence and Intracellular Multiplication. J. Bacteriol. 182: 4849-4855 [Abstract] [Full Text]  
  • Vemulapalli, R., He, Y., Boyle, S. M., Sriranganathan, N., Schurig, G. G. (2000). Brucella abortus Strain RB51 as a Vector for Heterologous Protein Expression and Induction of Specific Th1 Type Immune Responses. Infect. Immun. 68: 3290-3296 [Abstract] [Full Text]  
  • Guzman-Verri, C., Chaves-Olarte, E., von Eichel-Streiber, C., Lopez-Goni, I., Thelestam, M., Arvidson, S., Gorvel, J.-P., Moreno, E. (2001). GTPases of the Rho Subfamily Are Required for Brucella abortus Internalization in Nonprofessional Phagocytes. DIRECT ACTIVATION OF Cdc42. J. Biol. Chem. 276: 44435-44443 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»