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Infect Immun, August 1998, p. 3981-3984, Vol. 66, No. 8
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Binding of the Helicobacter pylori Vacuolating Cytotoxin to Target Cells

Paola Massari, Roberto Manetti, Daniela Burroni, Sandra Nuti, Nathalie Norais, Rino Rappuoli, and John L. Telford*

IRIS, Chiron Vaccines, 53100 Siena, Italy

Received 14 November 1997/Returned for modification 6 January 1998/Accepted 10 February 1998

The vacuolating cytotoxin of Helicobacter pylori, VacA, enters the cytoplasm of target cells and causes vacuolar degeneration by interfering with late stages of endocytosis. By using indirect immunofluorescence and flow cytometry, we have demonstrated that VacA binds to specific high-affinity cell surface receptors and that this interaction is necessary for cell intoxication.

* Corresponding author. Mailing address: IRIS, Chiron Vaccines, Via Fiorentina 1, 53100 Siena, Italy. Phone: 39 577 243470. Fax: 39 577 243564. E-mail: Telford{at}iris02.biocine.it.

Infect Immun, August 1998, p. 3981-3984, Vol. 66, No. 8
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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