This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chirhart-Gilleland, R. L. Articles by Roop, R. M. Search for Related Content PubMed PubMed Citation Articles by Chirhart-Gilleland, R. L. Articles by Roop, R. M., II

 Previous Article  |  Next Article 

Infect Immun, August 1998, p. 4000-4003, Vol. 66, No. 8
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification and Characterization of a 14-Kilodalton Brucella abortus Protein Reactive with Antibodies from Naturally and Experimentally Infected Hosts and T Lymphocytes from Experimentally Infected BALB/c Mice

Rebecca L. Chirhart-Gilleland, Michael E. Kovach, Philip H. Elzer, Stephen R. Jennings, and R. Martin Roop II^*

Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport, Louisiana 71130-3932

Received 11 February 1998/Returned for modification 22 April 1998/Accepted 27 May 1998

A low-molecular-weight recombinant Brucella abortus protein reactive with antibodies from a variety of naturally and experimentally infected hosts and T lymphocytes from experimentally infected mice was identified and given the designation BA14K. The gene encoding BA14K was cloned and characterized, and the predicted amino acid sequence of this immunoreactive protein showed no significant homology with previously described proteins. Sequences homologous to the cloned fragment encoding BA14K were identified by Southern blot analysis of genomic DNAs from representatives of all of the currently recognized Brucella species. Studies employing BA14K should contribute to our efforts to better understand the antigenic specificity of protective immunity to brucellosis.

---

^* Corresponding author. Mailing address: Department of Microbiology and Immunology, Louisiana State University Medical Center, P.O. Box 33932, 1501 Kings Highway, Shreveport, LA 71130-3932. Phone: (318) 675-5771. Fax: (318) 675-5764. E-mail: rroop{at}lsumc.edu.

Present address: Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294.

Present address: Department of Veterinary Science, Louisiana State University Agricultural Center, Baton Rouge, LA 70803.

---

Infect Immun, August 1998, p. 4000-4003, Vol. 66, No. 8
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

• Vemulapalli, T. H., Vemulapalli, R., Schurig, G. G., Boyle, S. M., Sriranganathan, N. (2006). Role in Virulence of a Brucella abortus Protein Exhibiting Lectin-Like Activity. Infect. Immun. 74: 183-191 [Abstract] [Full Text]  
• Kwaasi, A A., Al-Mohanna, F A, Nakeeb, S M, Roberts, G T, Al-Thawadi, S, Hassan, A Y, Al-Hokail, A, Elfaki, M G (2005). Correlation of antigenic expression with progress in antibiotic therapy of acute human brucellosis. J Med Microbiol 54: 533-538 [Abstract] [Full Text]  
• Ko, J., Splitter, G. A. (2003). Molecular Host-Pathogen Interaction in Brucellosis: Current Understanding and Future Approaches to Vaccine Development for Mice and Humans. Clin. Microbiol. Rev. 16: 65-78 [Abstract] [Full Text]  
• ROSINHA, G. M. S., MYIOSHI, A., AZEVEDO, V., SPLITTER, G. A., OLIVEIRA, S. C. (2002). Molecular and immunological characterisation of recombinant Brucella abortus glyceraldehyde-3-phosphate-dehydrogenase, a T- and B-cell reactive protein that induces partial protection when co-administered with an interleukin-12-expressing plasmid in a DNA vaccine formulation. J Med Microbiol 51: 661-671 [Abstract] [Full Text]