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Infection and Immunity, September 1998, p. 4061-4067, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of an Acidic-pH-Inducible Stress Protein (hsp70), a Putative Sulfatide Binding Adhesin, from Helicobacter pylori

Mario Huesca,1,2 Avery Goodwin,3 Arianna Bhagwansingh,2 Paul Hoffman,3 and Clifford A. Lingwood1,2,4,*

Research Institute, The Hospital for Sick Children,1 and Departments of Laboratory Medicine and Pathobiology2 and Biochemistry,4 University of Toronto, Toronto, Ontario, and Department of Microbiology, Dalhousie University, Halifax, Nova Scotia,3 Canada

Received 19 February 1998/Returned for modification 17 April 1998/Accepted 4 June 1998

The in vitro glycolipid binding specificity of the gastric pathogen Helicobacter pylori is altered to include sulfated glycolipids (sulfatides) following brief exposure of the organism to acid pH typical of the stomach. This change is prevented by anti-hsp70 antibodies, suggesting that hsp70 may be a stress-induced surface adhesin, mediating sulfatide recognition. To facilitate investigation of the role of hsp70 in attachment, we have cloned and sequenced the H. pylori hsp70 gene (dnaK). The hsp70 gene was identified by probing a cosmid DNA library made from H. pylori 439 with a PCR amplicon generated with oligonucleotides synthesized to highly conserved regions of dnaK. The 1.9-kb H. pylori hsp70 gene encodes a product of 616 amino acids. Primer extension analysis revealed a single transcription start site, while Northern blot analysis established that hsp70 was preferentially induced by low pH rather than by heat shock. The ability of H. pylori to alter its glycolipid binding specificity following exposure to low pH by upregulating hsp70 and by expressing hsp70 on the bacterial surface may provide a survival advantage during periods of high acid stress.

* Corresponding author. Mailing address: Research Institute, The Hospital for Sick Children, 555 University Ave., Toronto, Ontario M5G 1X8, Canada. Phone: (416) 813-5998. Fax: (416) 813-5993. E-mail: cling{at}sickkids.on.ca.

Infection and Immunity, September 1998, p. 4061-4067, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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