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Infection and Immunity, September 1998, p. 4151-4157, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Low Endotoxic Potential of Legionella pneumophila Lipopolysaccharide due to Failure of Interaction with the Monocyte Lipopolysaccharide Receptor CD14

B. Neumeister,1,* M. Faigle,1 M. Sommer,1 U. Zähringer,2 F. Stelter,3 R. Menzel,3 C. Schütt,3 and H. Northoff1

Abteilung Transfusionsmedizin der Universität Tübingen, AG Infektionsimmunologie, D-72076 Tübingen,1 Forschungszentrum Borstel, D-23845 Borstel,2 and Institut für Immunologie und Transfusionsmedizin der Universität Greifswald, D-17487 Greifswald,3 Germany

Received 17 February 1998/Returned for modification 27 March 1998/Accepted 1 May 1998

Legionella pneumophila, a gram-negative bacterium causing Legionnaires' disease and Pontiac fever, was shown to be highly reactive in in vitro gelation of Limulus lysate but not able to induce fever and the local Shwartzman reaction in rabbits and mice. We analyzed the capacity of purified L. pneumophila lipopolysaccharide (LPS-Lp) to induce activation of the human monocytic cell line Mono Mac 6, as revealed by secretion of proinflammatory cytokines and desensitization to subsequent LPS stimulation. We showed that despite normal reactivity of LPS-Lp in the Limulus amoebocyte lysate assay, induction of cytokine secretion in Mono Mac 6 cells and desensitization to an endotoxin challenge required LPS-Lp concentrations 1,000 times higher than for LPS of Salmonella enterica serovar Minnesota. Therefore, we examined the interaction of LPS-Lp with the LPS receptor CD14. We demonstrated that LPS-Lp did not bind to membrane-bound CD14 expressed on transfected CHO cells, nor did it react with soluble CD14. Our results suggest that the low endotoxic potential of LPS-Lp is due to a failure of interaction with the LPS receptor CD14.

* Corresponding author. Mailing address: Abteilung Transfusionsmedizin, Hoppe-Seyler-Straße 3, Universität Tübingen, D-72076 Tübingen, Germany. Phone: 49 7071 296658. Fax: 49 7071 295240. E-mail: Birgid.Neumeister{at}med.uni-tuebingen.de.

Infection and Immunity, September 1998, p. 4151-4157, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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