This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dubois, A.
Right arrow Articles by Monath, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dubois, A.
Right arrow Articles by Monath, T.

 Previous Article  |  Next Article 

Infection and Immunity, September 1998, p. 4340-4346, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Immunization against Natural Helicobacter pylori Infection in Nonhuman Primates

Andre Dubois,1,* Cynthia K. Lee,2 Nancy Fiala,1 Harry Kleanthous,2 Patrick T. Mehlman,1 and Thomas Monath2

Laboratory of Gastrointestinal and Liver Studies, Digestive Diseases Division, Department of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland,1 and OraVax, Inc., Cambridge, Massachusetts2

Received 12 January 1998/Returned for modification 24 April 1998/Accepted 8 June 1998

Helicobacter pylori infection is widespread in some breeding groups of a rhesus monkey colony (71% H. pylori positive by 1 year), and the rate of seroconversion is also high. As a result, these groups can be used to test the safety and efficacy of an anti-H. pylori vaccine. Nine-month-old female animals were randomized to receive either 8 mg of recombinant urease (rUre) plus 25 µg of Escherichia coli heat-labile enterotoxin (LT) (n = 26) or placebo plus LT (n = 29), given four times at 1-week intervals followed by a booster 1 month later. Ten months after the start of the immunization, the animals were subjected to endoscopy and biopsy samples were obtained. H. pylori negativity was defined as no H. pylori growth by culture and no H. pylori observed at histology. By this criterion, 2 (7%) of 29 animals receiving placebo and 8 (31%) of 26 immunized animals were H. pylori negative (P < 0.035). In addition, antral gastritis score was significantly less in H. pylori-negative immunized monkeys than in H. pylori-positive animals, whether they were given rUre plus LT or placebo plus LT (P < 0.02 or P < 0.01, respectively). Interestingly, antral gastritis was also significantly less in H. pylori-positive animals given rUre plus LT than in H. pylori-positive animals given placebo plus LT (P < 0.02). However, quantitative cultures did not demonstrate significant differences between the two latter groups. It is concluded that oral administration of rUre vaccine plus LT significantly protects nonhuman primates against H. pylori infection while not causing undesirable side effects.

* Corresponding author. Mailing address: Department of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814-4799. Phone: (301) 295-3607. Fax: (301) 295-3676 or -3557. E-mail: ADUBOIS{at}USUHS.MIL.

Infection and Immunity, September 1998, p. 4340-4346, Vol. 66, No. 9
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Agarwal, K., Agarwal, S. (2008). Helicobacter pylori Vaccine: From Past to Future. Mayo Clin Proc. 83: 169-175 [Abstract] [Full Text]  
  • Kusters, J. G., van Vliet, A. H. M., Kuipers, E. J. (2006). Pathogenesis of Helicobacter pylori Infection. Clin. Microbiol. Rev. 19: 449-490 [Abstract] [Full Text]  
  • Rossi, G., Ruggiero, P., Peppoloni, S., Pancotto, L., Fortuna, D., Lauretti, L., Volpini, G., Mancianti, S., Corazza, M., Taccini, E., Di Pisa, F., Rappuoli, R., Del Giudice, G. (2004). Therapeutic Vaccination against Helicobacter pylori in the Beagle Dog Experimental Model: Safety, Immunogenicity, and Efficacy. Infect. Immun. 72: 3252-3259 [Abstract] [Full Text]  
  • Yamaguchi, H., Osaki, T., Taguchi, H., Sato, N., Toyoda, A., Takahashi, M., Kai, M., Nakata, N., Komatsu, A., Atomi, Y., Kamiya, S. (2003). Effect of Bacterial Flora on Postimmunization Gastritis following Oral Vaccination of Mice with Helicobacter pylori Heat Shock Protein 60. CVI 10: 808-812 [Abstract] [Full Text]  
  • Shin, J.-H., Yang, M., Nam, S. W., Kim, J. T., Myung, N. H., Bang, W.-G., Roe, I. H. (2002). Use of Egg Yolk-Derived Immunoglobulin as an Alternative to Antibiotic Treatment for Control of Helicobacter pylori Infection. CVI 9: 1061-1066 [Abstract] [Full Text]  
  • Londono-Arcila, P., Freeman, D., Kleanthous, H., O'Dowd, A. M., Lewis, S., Turner, A. K., Rees, E. L., Tibbitts, T. J., Greenwood, J., Monath, T. P., Darsley, M. J. (2002). Attenuated Salmonella enterica Serovar Typhi Expressing Urease Effectively Immunizes Mice against Helicobacter pylori Challenge as Part of a Heterologous Mucosal Priming-Parenteral Boosting Vaccination Regimen. Infect. Immun. 70: 5096-5106 [Abstract] [Full Text]  
  • Hansen, L. M., Solnick, J. V. (2001). Selection for Urease Activity during Helicobacter pylori Infection of Rhesus Macaques (Macaca mulatta). Infect. Immun. 69: 3519-3522 [Abstract] [Full Text]  
  • Eaton, K. A., Mefford, M. E. (2001). Cure of Helicobacter pylori Infection and Resolution of Gastritis by Adoptive Transfer of Splenocytes in Mice. Infect. Immun. 69: 1025-1031 [Abstract] [Full Text]  
  • Solnick, J. V., Schauer, D. B. (2001). Emergence of Diverse Helicobacter Species in the Pathogenesis of Gastric and Enterohepatic Diseases. Clin. Microbiol. Rev. 14: 59-97 [Abstract] [Full Text]  
  • Meyer, F., Wilson, K. T., James, S. P. (2000). Modulation of Innate Cytokine Responses by Products of Helicobacter pylori. Infect. Immun. 68: 6265-6272 [Abstract] [Full Text]  
  • OLIARO, J., JOHNSON, R. D., CHEN, W., CHADWICK, V. S., MURRAY, A. (2000). Identification of an immunogenic 18-kDa protein of Helicobacter pylori by alkaline phosphatase gene fusions. J Med Microbiol 49: 643-650 [Abstract] [Full Text]  
  • RUIZ-BUSTOS, E., SIERRA-BELTRAN, A., ROMERO, M.J., RODRIGUEZ-JARAMILLO, C., ASCENCIO, F. (2000). Protection of BALB/c mice against experimental Helicobacter pylori infection by oral immunisation with H. pylori heparan sulphate-binding proteins coupled to cholera toxin {beta}-subunit. J Med Microbiol 49: 535-541 [Abstract] [Full Text]  
  • Solnick, J. V., Canfield, D. R., Hansen, L. M., Torabian, S. Z. (2000). Immunization with Recombinant Helicobacter pylori Urease in Specific-Pathogen-Free Rhesus Monkeys (Macaca mulatta). Infect. Immun. 68: 2560-2565 [Abstract] [Full Text]  
  • Weltzin, R., Guy, B., Thomas, W. D. Jr., Giannasca, P. J., Monath, T. P. (2000). Parenteral Adjuvant Activities of Escherichia coli Heat-Labile Toxin and Its B Subunit for Immunization of Mice against Gastric Helicobacter pylori Infection. Infect. Immun. 68: 2775-2782 [Abstract] [Full Text]  
  • Esteves, M. I., Schrenzel, M. D., Marini, R. P., Taylor, N. S., Xu, S., Hagen, S., Feng, Y., Shen, Z., Fox, J. G. (2000). Helicobacter pylori Gastritis in Cats with Long-Term Natural Infection as a Model of Human Disease. Am. J. Pathol. 156: 709-721 [Abstract] [Full Text]  
  • Dieterich, C., Bouzourene, H., Blum, A. L., Corthesy-Theulaz, I. E. (1999). Urease-Based Mucosal Immunization against Helicobacter heilmannii Infection Induces Corpus Atrophy in Mice. Infect. Immun. 67: 6206-6209 [Abstract] [Full Text]  
  • Covacci, A., Telford, J. L., Giudice, G. D., Parsonnet, J., Rappuoli, R. (1999). Helicobacter pylori Virulence and Genetic Geography. Science 284: 1328-1333 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»