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Infection and Immunity, September 1998, p. 4560-4563, Vol. 66, No. 9
0019-9567/98/$00.00+0

Escherichia coli O157:H7 Requires Intimin for Enteropathogenicity in Calves

Evelyn A. Dean-Nystrom,1,* Brad T. Bosworth,1 Harley W. Moon,2 and Alison D. O'Brien3

Enteric Diseases and Food Safety Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 500101; Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State University, Ames, Iowa 500112; and Department of Microbiology and Immunology, F. Edward Hébert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland 208143

Received 8 April 1998/Returned for modification 15 May 1998/Accepted 26 June 1998

Enterohemorrhagic Escherichia coli (EHEC) strains require intimin to induce attaching and effacing (A/E) lesions in newborn piglets. Infection of newborn calves with intimin-positive or intimin-negative EHEC O157:H7 demonstrated that intimin is needed for colonization, A/E lesions, and disease in cattle. These results suggest that experiments to determine if intimin-based vaccines reduce O157:H7 levels in cattle are warranted.

* Corresponding author. Mailing address: USDA, ARS, National Animal Disease Center, P.O. Box 70, Ames, IA 50010-0070. Phone: (515) 239-8376. Fax: (515) 239-8458. E-mail: enystrom{at}nadc.ars.usda.gov.

Infection and Immunity, September 1998, p. 4560-4563, Vol. 66, No. 9
0019-9567/98/$00.00+0


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