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Infection and Immunity, January 1999, p. 279-285, Vol. 67, No. 1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Role of Gamma Interferon in Helicobacter
pylori-Induced Gastric Inflammatory Responses in a Mouse
Model
Naoki
Sawai,1,*
Masakazu
Kita,2
Tadashi
Kodama,1
Toshihito
Tanahashi,1
Yoshio
Yamaoka,1
Yoh-ichi
Tagawa,3
Yoichiro
Iwakura,3 and
Jiro
Imanishi2
Third Department of Internal
Medicine1 and
Department of
Microbiology,2 Kyoto Prefectural University
of Medicine, Kyoto 602-0841, and
Laboratory Animal Research
Center, Institute of Medical Science, University of Tokyo, Tokyo
108-8639,3 Japan
Received 28 May 1998/Returned for modification 29 July
1998/Accepted 22 October 1998
The immune responses to Helicobacter pylori infection
play important roles in gastroduodenal diseases. The contribution of gamma interferon (IFN-
) to the immune responses, especially to the
induction of gastric inflammation and to protection from H. pylori infection, was investigated with IFN-
gene knockout
(IFN-
/
) mice. We first examined the colonizing
abilities of eight H. pylori strains with a short-term
infection test in order to select H. pylori strains which
could colonize the mouse stomach. Only three strains (ATCC 43504, CPY2052, and HPK127) colonized C57BL/6 wild-type mice, although all of
the strains except for ATCC 51110 could colonize
IFN-
/
mice. The number of H. pylori
organisms colonizing the stomach in wild-type mice was lower than that
in IFN-
/
mice. Oral immunization with the CPY2052
sonicate and cholera toxin protected against infection with strain
CPY2052 in both types of mouse. These findings suggested that IFN-
may play a protective role in H. pylori infection, although
the degree of its protective ability was estimated to be low. In
contrast, in a long-term infection test done to examine the
contribution of IFN-
to gastric inflammation, CPY2052-infected
wild-type mice developed a severe infiltration of mononuclear cells in
the lamina propria and erosions in the gastric epithelium 15 months
after infection, whereas CPY2052-infected IFN-
/
mice
showed no inflammatory symptoms. This result clearly demonstrated that
IFN-
plays an important role in the induction of gastric inflammation caused by H. pylori infection.
*
Corresponding author. Mailing address: Third Department
of Internal Medicine, Kyoto Prefectural University of Medicine,
Kawaramachi, Hirokoji, Kamigyo-ku, Kyoto 602-0841, Japan. Phone:
81-75-251-5519. Fax: 81-75-251-0710. E-mail:
sawai{at}basic.kpu-m.ac.jp.
Infection and Immunity, January 1999, p. 279-285, Vol. 67, No. 1
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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