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Infection and Immunity, January 1999, p. 88-93, Vol. 67, No. 1
0019-9567/99/$00.00+0

Campylobacter jejuni-Stimulated Secretion of Interleukin-8 by INT407 Cells

Thomas E. Hickey, Shihida Baqar, A. Louis Bourgeois, Cheryl P. Ewing, and Patricia Guerry*

Enteric Diseases Program, Naval Medical Research Center, Bethesda, Maryland

Received 17 July 1998/Returned for modification 16 September 1998/Accepted 15 October 1998

Incubation of INT407 cells with various clinical isolates of Campylobacter jejuni resulted in secretion of interleukin-8 (IL-8) at levels ranging from 96 to 554 pg/ml at 24 h. The strains which produced the highest levels of IL-8 secretion were 81-176 and BT44. Induction of IL-8 secretion required live cells of 81-176 and was dependent on de novo protein synthesis. Site-specific mutants of 81-176, which were previously shown to be defective in adherence and invasion, resulted in reduced levels of secretion of IL-8, and cheY mutants of strains 81-176 and 749, which are hyperadherent and hyperinvasive, resulted in higher levels of IL-8 secretion. Another mutant of 81-176, which adheres at about 43% of the wild-type levels but is noninvasive, also showed marked reduction in IL-8 levels, suggesting that invasion is necessary for high levels of IL-8 secretion. When gentamicin was added to INT407 cells at 2 h after infection with 81-176, IL-8 secretion 22 h later was equivalent to that of controls without gentamicin, suggesting that the events which trigger induction and release of IL-8 occur early in the interactions of bacteria and eukaryotic cells.

* Corresponding author. Mailing address: 12300 Washington Ave., Rockville, MD 20852. Phone: (301) 295-1514. Fax: (301) 295-6171. E-mail: guerryp{at}nmripo.nmri.nnmc.navy.mil.

Infection and Immunity, January 1999, p. 88-93, Vol. 67, No. 1
0019-9567/99/$00.00+0


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