This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schraw, W.
Right arrow Articles by Cover, T. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schraw, W.
Right arrow Articles by Cover, T. L.

 Previous Article  |  Next Article 

Infection and Immunity, October 1999, p. 5247-5252, Vol. 67, No. 10
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Kinetics and Mechanisms of Extracellular Protein Release by Helicobacter pylori

Wayne Schraw,1 Mark S. McClain,1 and Timothy L. Cover1,2,3,*

Division of Infectious Diseases, Department of Medicine,1 and Department of Microbiology and Immunology,2 Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2605, and Department of Veterans Affairs Medical Center, Nashville, Tennessee 37232-26373

Received 9 April 1999/Returned for modification 24 May 1999/Accepted 16 July 1999

To investigate the kinetics and mechanisms of extracellular protein release by Helicobacter pylori, we analyzed the entry of metabolically radiolabeled bacterial proteins into broth culture supernatant. At early time points, vacuolating cytotoxin (VacA) constituted a major extracellular protein. Subsequently, culture supernatants accumulated many proteins that were components of intact bacterial cells. This nonselective release of proteins was associated with a decreasing turbidity of cultures and loss of bacterial viability, indicative of an autolytic process. The rates of VacA secretion and autolysis were each influenced by medium composition, and therefore these may be regulated phenomena. Extracellular release of proteins by H. pylori may be an important adaptation that facilitates the persistence of H. pylori in the human gastric mucus layer. Moreover, entry of proinflammatory proteins into the gastric mucosa may contribute to the induction of a mucosal inflammatory response.

* Corresponding author. Mailing address: Division of Infectious Diseases, A3310 Medical Center North, Vanderbilt University School of Medicine, Nashville, TN 37232-2605. Phone: (615) 322-2035. Fax: (615) 343-6160. E-mail: COVERTL{at}ctrvax.vanderbilt.edu.

Infection and Immunity, October 1999, p. 5247-5252, Vol. 67, No. 10
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Nossa, C. W., Blanke, S. R. (2006). Modification of a Mammalian Cell Protein in the Presence of [32P-Adenylate]NAD: Evidence for ADP Ribosylation Activity Associated with Helicobacter pylori.. Infect. Immun. 74: 3071-3076 [Abstract] [Full Text]  
  • Fitchen, N., Letley, D. P, O'Shea, P., Atherton, J. C, Williams, P., Hardie, K. R (2005). All subtypes of the cytotoxin VacA adsorb to the surface of Helicobacter pylori post-secretion. J Med Microbiol 54: 621-630 [Abstract] [Full Text]  
  • Schmausser, B., Josenhans, C., Endrich, S., Suerbaum, S., Sitaru, C., Andrulis, M., Brandlein, S., Rieckmann, P., Muller-Hermelink, H. K., Eck, M. (2004). Downregulation of CXCR1 and CXCR2 Expression on Human Neutrophils by Helicobacter pylori: a New Pathomechanism in H. pylori Infection?. Infect. Immun. 72: 6773-6779 [Abstract] [Full Text]  
  • Kim, N., Weeks, D. L., Shin, J. M., Scott, D. R., Young, M. K., Sachs, G. (2002). Proteins Released by Helicobacter pylori In Vitro. J. Bacteriol. 184: 6155-6162 [Abstract] [Full Text]  
  • Bumann, D., Aksu, S., Wendland, M., Janek, K., Zimny-Arndt, U., Sabarth, N., Meyer, T. F., Jungblut, P. R. (2002). Proteome Analysis of Secreted Proteins of the Gastric Pathogen Helicobacter pylori. Infect. Immun. 70: 3396-3403 [Abstract] [Full Text]  
  • Gobert, A. P., Mersey, B. D., Cheng, Y., Blumberg, D. R., Newton, J. C., Wilson, K. T. (2002). Cutting Edge: Urease Release by Helicobacter pylori Stimulates Macrophage Inducible Nitric Oxide Synthase. J. Immunol. 168: 6002-6006 [Abstract] [Full Text]  
  • Testerman, T. L., McGee, D. J., Mobley, H. L. T. (2001). Helicobacter pylori Growth and Urease Detection in the Chemically Defined Medium Ham's F-12 Nutrient Mixture. J. Clin. Microbiol. 39: 3842-3850 [Abstract] [Full Text]  
  • Tullius, M. V., Harth, G., Horwitz, M. A. (2001). High Extracellular Levels of Mycobacterium tuberculosis Glutamine Synthetase and Superoxide Dismutase in Actively Growing Cultures Are Due to High Expression and Extracellular Stability Rather than to a Protein-Specific Export Mechanism. Infect. Immun. 69: 6348-6363 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»