This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tobe, T.
Right arrow Articles by Sasakawa, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tobe, T.
Right arrow Articles by Sasakawa, C.

 Previous Article  |  Next Article 

Infection and Immunity, October 1999, p. 5455-5462, Vol. 67, No. 10
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Complete DNA Sequence and Structural Analysis of the Enteropathogenic Escherichia coli Adherence Factor Plasmid

Toru Tobe,1,* Tetsuya Hayashi,2 Chang-Gyun Han,3 Gary K. Schoolnik,4 Eiichi Ohtsubo,3 and Chihiro Sasakawa1,5

Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minatoku, Tokyo 108-8639,1 Department of Bacteriology, Shinshu University School of Medicine, Asahi 3-1-2, Matsumoto 390-8621,2 Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyoku, Tokyo 113,3 and Department of Bacterial Toxinology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565,5 Japan, and Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford California 943054

Received 30 April 1999/Returned for modification 18 June 1999/Accepted 9 July 1999

The complete nucleotide sequence and organization of the enteropathogenic Escherichia coli (EPEC) adherence factor (EAF) plasmid of EPEC strain B171 (O111:NM) were determined. The EAF plasmid encodes two known virulence-related operons, the bfp operon, which is composed of genes necessary for biosynthesis of bundle-forming pili, and the bfpTVW (perABC) operon, composed of regulatory genes required for bfp transcription and also for transcriptional activation of the eae gene in the LEE pathogenicity island on the EPEC chromosome. The 69-kb EAF plasmid, henceforth designated pB171, contains, besides the bfp and bfpTVW (perABC) operons, potential virulence-associated genes, plasmid replication and maintenance genes, and many insertion sequence elements. Of the newly identified open reading frames (ORFs), two which comprise a single operon had the potential to encode proteins with high similarity to a C-terminal region of ToxB whose coding sequence is located on pO157, a large plasmid harbored by enterohemorrhagic E. coli. Another ORF, located between the bfp and bfpTVW operons, showed high similarity with trcA, a bfpT-regulated chaperone-like protein gene of EPEC. Two sites were found to be putative replication regions: one similar to RepFIIA of p307 or F, and the other similar to RepFIB of R100 (NR1). In addition, we identified a third region that contains plasmid maintenance genes. Insertion elements were scattered throughout the plasmid, indicating the mosaic nature of the EAF plasmid and suggesting evolutionary events by which virulence genes may have been obtained.

* Corresponding author. Mailing address: Department of Bacteriology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minatoku, Tokyo 108-8639, Japan. Phone: 81-3-5449-5537. Fax: 81-3-5449-5405. E-mail: torutobe{at}ims.u-tokyo.ac.jp.

Infection and Immunity, October 1999, p. 5455-5462, Vol. 67, No. 10
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Cheng, C., Tennant, S. M., Azzopardi, K. I., Bennett-Wood, V., Hartland, E. L., Robins-Browne, R. M., Tauschek, M. (2009). Contribution of the pst-phoU Operon to Cell Adherence by Atypical Enteropathogenic Escherichia coli and Virulence of Citrobacter rodentium. Infect. Immun. 77: 1936-1944 [Abstract] [Full Text]  
  • Girardeau, J. P., Bertin, Y., Martin, C. (2009). Genomic analysis of the PAI ICL3 locus in pathogenic LEE-negative Shiga toxin-producing Escherichia coli and Citrobacter rodentium. Microbiology 155: 1016-1027 [Abstract] [Full Text]  
  • Salinger, N., Kokona, B., Fairman, R., Okeke, I. N. (2009). The Plasmid-Encoded Regulator Activates Factors Conferring Lysozyme Resistance on Enteropathogenic Escherichia coli Strains. Appl. Environ. Microbiol. 75: 275-280 [Abstract] [Full Text]  
  • Ogura, Y., Abe, H., Katsura, K., Kurokawa, K., Asadulghani, M., Iguchi, A., Ooka, T., Nakayama, K., Yamashita, A., Hattori, M., Tobe, T., Hayashi, T. (2008). Systematic Identification and Sequence Analysis of the Genomic Islands of the Enteropathogenic Escherichia coli Strain B171-8 by the Combined Use of Whole-Genome PCR Scanning and Fosmid Mapping. J. Bacteriol. 190: 6948-6960 [Abstract] [Full Text]  
  • Rasko, D. A., Rosovitz, M. J., Myers, G. S. A., Mongodin, E. F., Fricke, W. F., Gajer, P., Crabtree, J., Sebaihia, M., Thomson, N. R., Chaudhuri, R., Henderson, I. R., Sperandio, V., Ravel, J. (2008). The Pangenome Structure of Escherichia coli: Comparative Genomic Analysis of E. coli Commensal and Pathogenic Isolates. J. Bacteriol. 190: 6881-6893 [Abstract] [Full Text]  
  • Tarr, C. L., Nelson, A. M., Beutin, L., Olsen, K. E. P., Whittam, T. S. (2008). Molecular Characterization Reveals Similar Virulence Gene Content in Unrelated Clonal Groups of Escherichia coli of Serogroup O174 (OX3). J. Bacteriol. 190: 1344-1349 [Abstract] [Full Text]  
  • Ringgaard, S., Lowe, J., Gerdes, K. (2007). Centromere Pairing by a Plasmid-encoded Type I ParB Protein. J. Biol. Chem. 282: 28216-28225 [Abstract] [Full Text]  
  • Nwaneshiudu, A. I., Mucci, T., Pickard, D. J., Okeke, I. N. (2007). A Second Large Plasmid Encodes Conjugative Transfer and Antimicrobial Resistance in O119:H2 and Some Typical O111 Enteropathogenic Escherichia coli Strains. J. Bacteriol. 189: 6074-6079 [Abstract] [Full Text]  
  • Joo, L. M., Macfarlane-Smith, L. R., Okeke, I. N. (2007). Error-Prone DNA Repair System in Enteroaggregative Escherichia coli Identified by Subtractive Hybridization. J. Bacteriol. 189: 3793-3803 [Abstract] [Full Text]  
  • Lacher, D. W., Steinsland, H., Blank, T. E., Donnenberg, M. S., Whittam, T. S. (2007). Molecular Evolution of Typical Enteropathogenic Escherichia coli: Clonal Analysis by Multilocus Sequence Typing and Virulence Gene Allelic Profiling. J. Bacteriol. 189: 342-350 [Abstract] [Full Text]  
  • Brinkley, C., Burland, V., Keller, R., Rose, D. J., Boutin, A. T., Klink, S. A., Blattner, F. R., Kaper, J. B. (2006). Nucleotide Sequence Analysis of the Enteropathogenic Escherichia coli Adherence Factor Plasmid pMAR7. Infect. Immun. 74: 5408-5413 [Abstract] [Full Text]  
  • Froehlich, B., Parkhill, J., Sanders, M., Quail, M. A., Scott, J. R. (2005). The pCoo Plasmid of Enterotoxigenic Escherichia coli Is a Mosaic Cointegrate. J. Bacteriol. 187: 6509-6516 [Abstract] [Full Text]  
  • Tozzoli, R., Caprioli, A., Morabito, S. (2005). Detection of toxB, a Plasmid Virulence Gene of Escherichia coli O157, in Enterohemorrhagic and Enteropathogenic E. coli. J. Clin. Microbiol. 43: 4052-4056 [Abstract] [Full Text]  
  • Fothergill, T. J. G., Barilla, D., Hayes, F. (2005). Protein Diversity Confers Specificity in Plasmid Segregation. J. Bacteriol. 187: 2651-2661 [Abstract] [Full Text]  
  • Froehlich, B., Holtzapple, E., Read, T. D., Scott, J. R. (2004). Horizontal Transfer of CS1 Pilin Genes of Enterotoxigenic Escherichia coli. J. Bacteriol. 186: 3230-3237 [Abstract] [Full Text]  
  • Rajanna, C., Wang, J., Zhang, D., Xu, Z., Ali, A., Hou, Y.-M., Karaolis, D. K. R. (2003). The Vibrio Pathogenicity Island of Epidemic Vibrio cholerae Forms Precise Extrachromosomal Circular Excision Products. J. Bacteriol. 185: 6893-6901 [Abstract] [Full Text]  
  • Ibarra, J. A., Villalba, M. I., Puente, J. L. (2003). Identification of the DNA Binding Sites of PerA, the Transcriptional Activator of the bfp and per Operons in Enteropathogenic Escherichia coli. J. Bacteriol. 185: 2835-2847 [Abstract] [Full Text]  
  • Tarr, C. L., Large, T. M., Moeller, C. L., Lacher, D. W., Tarr, P. I., Acheson, D. W., Whittam, T. S. (2002). Molecular Characterization of a Serotype O121:H19 Clone, a Distinct Shiga Toxin-Producing Clone of Pathogenic Escherichia coli. Infect. Immun. 70: 6853-6859 [Abstract] [Full Text]  
  • Ramer, S. W., Schoolnik, G. K., Wu, C.-Y., Hwang, J., Schmidt, S. A., Bieber, D. (2002). The Type IV Pilus Assembly Complex: Biogenic Interactions among the Bundle-Forming Pilus Proteins of Enteropathogenic Escherichia coli. J. Bacteriol. 184: 3457-3465 [Abstract] [Full Text]  
  • d'Hauteville, H., Khan, S., Maskell, D. J., Kussak, A., Weintraub, A., Mathison, J., Ulevitch, R. J., Wuscher, N., Parsot, C., Sansonetti, P. J. (2002). Two msbB Genes Encoding Maximal Acylation of Lipid A Are Required for Invasive Shigella flexneri to Mediate Inflammatory Rupture and Destruction of the Intestinal Epithelium. J. Immunol. 168: 5240-5251 [Abstract] [Full Text]  
  • Ebersbach, G., Gerdes, K. (2001). The double par locus of virulence factor pB171: DNA segregation is correlated with oscillation of ParA. Proc. Natl. Acad. Sci. USA 98: 15078-15083 [Abstract] [Full Text]  
  • Tatsuno, I., Horie, M., Abe, H., Miki, T., Makino, K., Shinagawa, H., Taguchi, H., Kamiya, S., Hayashi, T., Sasakawa, C. (2001). toxB Gene on pO157 of Enterohemorrhagic Escherichiacoli O157:H7 Is Required for Full Epithelial Cell Adherence Phenotype. Infect. Immun. 69: 6660-6669 [Abstract] [Full Text]  
  • Deng, W., Li, Y., Vallance, B. A., Finlay, B. B. (2001). Locus of Enterocyte Effacement from Citrobacter rodentium: Sequence Analysis and Evidence for Horizontal Transfer among Attaching and Effacing Pathogens. Infect. Immun. 69: 6323-6335 [Abstract] [Full Text]  
  • Okeke, I. N., Borneman, J. A., Shin, S., Mellies, J. L., Quinn, L. E., Kaper, J. B. (2001). Comparative Sequence Analysis of the Plasmid-Encoded Regulator of Enteropathogenic Escherichia coli Strains. Infect. Immun. 69: 5553-5564 [Abstract] [Full Text]  
  • Han, C.-G., Shiga, Y., Tobe, T., Sasakawa, C., Ohtsubo, E. (2001). Structural and Functional Characterization of IS679 and IS66-Family Elements. J. Bacteriol. 183: 4296-4304 [Abstract] [Full Text]  
  • Dobrindt, U., Blum-Oehler, G., Hartsch, T., Gottschalk, G., Ron, E. Z., Funfstuck, R., Hacker, J. (2001). S-Fimbria-Encoding Determinant sfaI Is Located on Pathogenicity Island III536 of Uropathogenic Escherichia coli Strain 536. Infect. Immun. 69: 4248-4256 [Abstract] [Full Text]  
  • Snellings, N. J., Popek, M., Lindler, L. E. (2001). Complete DNA Sequence of Yersinia enterocolitica Serotype 0:8 Low-Calcium-Response Plasmid Reveals a New Virulence Plasmid-Associated Replicon. Infect. Immun. 69: 4627-4638 [Abstract] [Full Text]  
  • Venkatesan, M. M., Goldberg, M. B., Rose, D. J., Grotbeck, E. J., Burland, V., Blattner, F. R. (2001). Complete DNA Sequence and Analysis of the Large Virulence Plasmid of Shigella flexneri. Infect. Immun. 69: 3271-3285 [Abstract] [Full Text]  
  • Sánchez-SanMartín, C., Bustamante, V. H., Calva, E., Puente, J. L. (2001). Transcriptional Regulation of the orf19 Gene and the tir-cesT-eae Operon of Enteropathogenic Escherichia coli. J. Bacteriol. 183: 2823-2833 [Abstract] [Full Text]  
  • Prentice, M. B., James, K. D., Parkhill, J., Baker, S. G., Stevens, K., Simmonds, M. N., Mungall, K. L., Churcher, C., Oyston, P. C. F., Titball, R. W., Wren, B. W., Wain, J., Pickard, D., Hien, T. T., Farrar, J. J., Dougan, G. (2001). Yersinia pestis pFra Shows Biovar-Specific Differences and Recent Common Ancestry with a Salmonella enterica Serovar Typhi Plasmid. J. Bacteriol. 183: 2586-2594 [Abstract] [Full Text]  
  • Zhu, C., Agin, T. S., Elliott, S. J., Johnson, L. A., Thate, T. E., Kaper, J. B., Boedeker, E. C. (2001). Complete Nucleotide Sequence and Analysis of the Locus of Enterocyte Effacement from Rabbit Diarrheagenic Escherichia coli RDEC-1. Infect. Immun. 69: 2107-2115 [Abstract] [Full Text]  
  • Haneda, T., Okada, N., Nakazawa, N., Kawakami, T., Danbara, H. (2001). Complete DNA Sequence and Comparative Analysis of the 50-Kilobase Virulence Plasmid of Salmonella enterica Serovar Choleraesuis. Infect. Immun. 69: 2612-2620 [Abstract] [Full Text]  
  • Blank, T. E., Zhong, H., Bell, A. L., Whittam, T. S., Donnenberg, M. S. (2000). Molecular Variation among Type IV Pilin (bfpA) Genes from Diverse Enteropathogenic Escherichia coli Strains. Infect. Immun. 68: 7028-7038 [Abstract] [Full Text]  
  • Dozois, C. M., Dho-Moulin, M., Bree, A., Fairbrother, J. M., Desautels, C., Curtiss, R. III (2000). Relationship between the Tsh Autotransporter and Pathogenicity of Avian Escherichia coli and Localization and Analysis of the tsh Genetic Region. Infect. Immun. 68: 4145-4154 [Abstract] [Full Text]  
  • Sherburne, C. K., Lawley, T. D., Gilmour, M. W., Blattner, F. R., Burland, V., Grotbeck, E., Rose, D. J., Taylor, D. E. (2000). The complete DNA sequence and analysis of R27, a large IncHI plasmid from Salmonella typhi that is temperature sensitive for transfer. Nucleic Acids Res 28: 2177-2186 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»