This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Takashiba, S.
Right arrow Articles by Shapira, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Takashiba, S.
Right arrow Articles by Shapira, L.

 Previous Article  |  Next Article 

Infection and Immunity, November 1999, p. 5573-5578, Vol. 67, No. 11
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Differentiation of Monocytes to Macrophages Primes Cells for Lipopolysaccharide Stimulation via Accumulation of Cytoplasmic Nuclear Factor kappa B

Shogo Takashiba,1 Thomas E. Van Dyke,2 Salomon Amar,2 Yoji Murayama,1 Aubrey W. Soskolne,3 and Lior Shapira3,*

Department of Periodontology and Endodontology, Okayama University Dental School, Okayama, Japan1; Department of Periodontology and Oral Biology, School of Dental Medicine, Boston University, Boston, Massachusetts2; and Department of Periodontology, The Hebrew University-Hadassah Faculty of Dental Medicine, Jerusalem, Israel3

Received 5 May 1999/Returned for modification 1 July 1999/Accepted 30 July 1999

During infection, circulating blood monocytes migrate from the vasculature to the extravascular compartments where they mature into tissue macrophages. The maturation process prepares the cell to actively participate in the inflammatory and the immune responses, and many transcription factors have been found to be involved. Here we report on a novel role for nuclear factor kappa B (NF-kappa B) in this process. Its accumulation in the cytoplasm of differentiated macrophages is responsible for the enhanced ability of the cell to respond to lipopolysaccharide (LPS) stimulation, as determined by tumor necrosis factor alpha (TNF-alpha ) secretion. Differentiation of the human monocytic cell line THP-1 into macrophage-like cells was induced by exposure of the cells to phorbol myristate acetate. DNA-bindable NF-kappa B was not detected in the cytoplasm of undifferentiated THP-1 cells but accumulated in the cytoplasm of the cells following differentiation. No TNF-alpha was detected in the media of resting differentiated and nondifferentiated THP-1 cells. Stimulation with LPS of differentiated cells induced the production of higher levels of TNF-alpha than stimulation of nondifferentiated cells. This hyperresponsiveness to LPS was found in the mRNA and secreted TNF-alpha levels. Furthermore, stimulation with LPS induced the translocation of NF-kappa B from the cytoplasm into the nucleus. This translocation process was more rapid in the differentiated cells than in the nondifferentiated cells, and the resultant accumulated levels of NF-kappa B in the nucleus were higher. The DNA-bindable NF-kappa B was identified as a heterodimer of p65 and p50. The results suggest that NF-kappa B accumulation in the cytoplasm during maturation of monocytes to macrophages primes the cells for enhanced responsiveness to LPS and results in the rapid secretion of inflammatory mediators, such as TNF-alpha , by mature macrophages following LPS challenge.

* Corresponding author. Mailing address: Department of Periodontology, The Hebrew University-Hadassah Faculty of Dental Medicine, P.O. Box 12272, Jerusalem 91120, Israel. Phone: 972-2-6777826 or 972-2-6777827. Fax: 972-2-6438705. E-mail: shapiral{at}cc.huji.ac.il.

Infection and Immunity, November 1999, p. 5573-5578, Vol. 67, No. 11
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Seshadri, S., Kannan, Y., Mitra, S., Parker-Barnes, J., Wewers, M. D. (2009). MAIL Regulates Human Monocyte IL-6 Production. J. Immunol. 183: 5358-5368 [Abstract] [Full Text]  
  • Gaurnier-Hausser, A., Rothman, V. L., Dimitrov, S., Tuszynski, G. P. (2008). The Novel Angiogenic Inhibitor, Angiocidin, Induces Differentiation of Monocytes to Macrophages. Cancer Res. 68: 5905-5914 [Abstract] [Full Text]  
  • Laza-Stanca, V., Stanciu, L. A., Message, S. D., Edwards, M. R., Gern, J. E., Johnston, S. L. (2006). Rhinovirus Replication in Human Macrophages Induces NF-{kappa}B-Dependent Tumor Necrosis Factor Alpha Production.. J. Virol. 80: 8248-8258 [Abstract] [Full Text]  
  • Bellaire, B. H, Roop, R. M. II, Cardelli, J. A. (2005). Opsonized Virulent Brucella abortus Replicates within Nonacidic, Endoplasmic Reticulum-Negative, LAMP-1-Positive Phagosomes in Human Monocytes. Infect. Immun. 73: 3702-3713 [Abstract] [Full Text]  
  • Anstead, G. M., Chandrasekar, B., Zhang, Q., Melby, P. C. (2003). Multinutrient undernutrition dysregulates the resident macrophage proinflammatory cytokine network, nuclear factor-{kappa}B activation, and nitric oxide production. J. Leukoc. Biol. 74: 982-991 [Abstract] [Full Text]  
  • OSTERUD, B., BJORKLID, E. (2003). Role of Monocytes in Atherogenesis. Physiol. Rev. 83: 1069-1112 [Abstract] [Full Text]  
  • Jones, M. A., Totemeyer, S., Maskell, D. J., Bryant, C. E., Barrow, P. A. (2003). Induction of Proinflammatory Responses in the Human Monocytic Cell Line THP-1 by Campylobacter jejuni. Infect. Immun. 71: 2626-2633 [Abstract] [Full Text]  
  • Chong, Y. H., Shin, Y. J., Suh, Y.-H. (2003). Cyclic AMP Inhibition of Tumor Necrosis Factor alpha Production Induced by Amyloidogenic C-Terminal Peptide of Alzheimer's Amyloid Precursor Protein in Macrophages: Involvement of Multiple Intracellular Pathways and Cyclic AMP Response Element Binding Protein. Mol. Pharmacol. 63: 690-698 [Abstract] [Full Text]  
  • Bierhaus, A., Wolf, J., Andrassy, M., Rohleder, N., Humpert, P. M., Petrov, D., Ferstl, R., von Eynatten, M., Wendt, T., Rudofsky, G., Joswig, M., Morcos, M., Schwaninger, M., McEwen, B., Kirschbaum, C., Nawroth, P. P. (2003). A mechanism converting psychosocial stress into mononuclear cell activation. Proc. Natl. Acad. Sci. USA 100: 1920-1925 [Abstract] [Full Text]  
  • El-Etr, S. H., Yan, L., Cirillo, J. D. (2001). Fish Monocytes as a Model for Mycobacterial Host-Pathogen Interactions. Infect. Immun. 69: 7310-7317 [Abstract] [Full Text]  
  • Rosenberger, C. M., Scott, M. G., Gold, M. R., Hancock, R. E. W., Finlay, B. B. (2000). Salmonella typhimurium Infection and Lipopolysaccharide Stimulation Induce Similar Changes in Macrophage Gene Expression. J. Immunol. 164: 5894-5904 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»