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Infection and Immunity, November 1999, p. 5863-5868, Vol. 67, No. 11
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Targeted Salivary Gland Immunization with Plasmid DNA Elicits Specific Salivary Immunoglobulin A and G Antibodies and Serum Immunoglobulin G Antibodies in Mice

Shigetada Kawabata,1,* Yutaka Terao,1 Taku Fujiwara,2 Ichiro Nakagawa,1 and Shigeyuki Hamada1

Departments of Oral Microbiology1 and Pedodontics,2 Osaka University Faculty of Dentistry, Suita-Osaka 565-0871, Japan

Received 19 July 1999/Returned for modification 16 August 1999/Accepted 23 August 1999

For the development of vaccines against oral and pharyngeal pathogens invading the mucosal epithelia, both secretory and serum immunoglobulin A (IgA) and IgG antibodies and cytotoxic T lymphocytes (CTL) have been induced. We used a novel approach, targeted salivary gland (TSG) immunization, using plasmid pcDNA3/fimA, coding for Porphyromonas gingivalis fimbriae. Expression of subunit protein, fimbrillin, was observed in eukaryotic cells growing in vitro following transfection with pcDNA3/fimA. In this study, we obtained good humoral and cell-mediated immune responses in BALB/c mice by TSG administration using the above-mentioned DNA vaccine. The production of fimbria-specific IgA and IgG antibodies in saliva and serum IgG antibody was significantly stimulated by TSG immunization. Injection of DNA vaccine into salivary gland elicited high-level production of antigen-specific IgG antibody, similar to that induced following intramuscular immunization. The major IgG subclass that recognized fimbriae was IgG2a in serum from pcDNA3/fimA-immunized mice. Reverse transcription-PCR analysis of mononuclear cells from salivary glands showed that levels of Th2 cytokine-specific mRNA were higher in the immunized group than in the nonimmunized group. In addition, TSG DNA immunization resulted in the generation of antigen-specific CTL in spleen. These results indicate that TSG immunization with plasmid DNA may represent a genetic immunization strategy against infection by oral and pharyngeal pathogens that may invade local, mucosal surfaces.

* Corresponding author. Mailing address: Department of Oral Microbiology, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita-Osaka 565-0871, Japan. Phone: 81-6-6879-2898. Fax: 81-6-6878-4755. E-mail: kawabata{at}dent.osaka-u.ac.jp.

Infection and Immunity, November 1999, p. 5863-5868, Vol. 67, No. 11
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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