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Infection and Immunity, November 1999, p. 5877-5884, Vol. 67, No. 11
0019-9567/99/$04.00+0

Protection of Mice against Brucellosis by Vaccination with Brucella melitensis WR201(16MDelta purEK)

David L. Hoover,1,* Robert M. Crawford,2,dagger Lillian L. Van De Verg,1,Dagger Mina J. Izadjoo,2 Apurba K. Bhattacharjee,1 Chrysanthi M. Paranavitana,1 Richard L. Warren,1,§ Mikeljon P. Nikolich,1 and Ted L. Hadfield3

Department of Bacterial Diseases, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100,1 and American Registry of Pathology2 and Department of Infectious and Parasitic Diseases,3 Armed Forces Institute of Pathology, Washington, D.C. 20306-6000

Received 12 April 1999/Returned for modification 23 June 1999/Accepted 30 August 1999

Human brucellosis can be acquired from infected animal tissues by ingestion, inhalation, or contamination of the conjunctiva or traumatized skin by infected animal products. A vaccine to protect humans from occupational exposure or from zoonotic infection in areas where the disease is endemic would reduce an important cause of morbidity worldwide. Vaccines currently used in animals are unsuitable for human use. We tested a live, attenuated, purine-auxotrophic mutant strain of Brucella melitensis, WR201, for its ability to elicit cellular and humoral immune responses and to protect mice against intranasal challenge with B. melitensis 16M. Mice inoculated intraperitoneally with WR201 made serum antibody to lipopolysaccharide and non-O-polysaccharide antigens. Splenocytes from immunized animals released interleukin-2 (IL-2), gamma interferon, and IL-10 when cultured with Brucella antigens. Immunization led to protection from disseminated infection but had only a slight effect on clearance of the challenge inoculum from the lungs. These studies suggest that WR201 should be further investigated as a vaccine to prevent human brucellosis.

* Corresponding author. Mailing address: Department of Bacterial Diseases, Walter Reed Army Institute of Research, Washington, DC 20307-5100. Phone: (301) 319-9573. Fax: (301) 319-9123. E-mail: david.hoover{at}na.amedd.army.mil.

dagger Present address: Hemagen Diagnostics, Inc., Columbia, MD 21045.

Dagger Present address: JVAP, Fort Detrick, MD 21702-5041.

§ Present address: Dugway Proving Grounds, Dugway, UT 84022.

Infection and Immunity, November 1999, p. 5877-5884, Vol. 67, No. 11
0019-9567/99/$04.00+0


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