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Infection and Immunity, December 1999, p. 6558-6564, Vol. 67, No. 12
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Influence of Environmental Conditions on Hydrogen Peroxide Formation by Streptococcus gordonii

John P. Barnard1 and Murray W. Stinson1,2,*

Center for Microbial Pathogenesis, Department of Microbiology, School of Medicine and Biomedical Sciences,1 and Department of Oral Biology, School of Dental Medicine,2 State University of New York at Buffalo, Buffalo, New York 14214-3000

Received 21 June 1999/Returned for modification 23 August 1999/Accepted 23 September 1999

Hydrogen peroxide generated by viridans group streptococci has an antagonistic effect on many bacterial species, including a number of pathogens, in the oral environment. This study examines the influence of a variety of environmental conditions on rates of hydrogen peroxide synthesis by Streptococcus gordonii. Hydrogen peroxide was synthesized at every concentration of glucose and sucrose tested from 10 µM to 1 M, with the highest rates occurring at 0.1 mM sucrose and 1 mM glucose. S. gordonii appeared to have an intracellular store of polysaccharide which supported hydrogen peroxide formation even when the assay buffer contained no carbohydrate. Most heavy metal ions inhibited peroxidogenesis, and anaerobic conditions induced adaptive down-regulation of hydrogen peroxide synthesis; however, peroxidogenesis was generally insensitive to moderate increases in salt concentration, alteration of the mineral content of the assay solution, and changes in pH between 5.0 and 7.5. In contrast, stimulation of peroxidogenesis occurred in 1 mM Mg2+ and 10 to 50 mM potassium L-lactate. Maximum peroxidogenesis occurred during the mid-logarithmic and late-logarithmic phases of bacterial growth. These bacterial responses may have significant implications for oral ecology and oral health.

* Corresponding author. Mailing address: Center for Microbial Pathogenesis, Department of Microbiology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, 3435 Main St., Buffalo, NY 14214-3000. Phone: (716) 829-2178. Fax: (716) 829-2158. E-mail: mstinson{at}acsu.buffalo.edu.

Infection and Immunity, December 1999, p. 6558-6564, Vol. 67, No. 12
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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